Construction of aptasensors for sensitive detection of 8-OH-dG based on a diffusion mediated electrochemiluminescence quenching effect

2020 ◽  
Vol 56 (75) ◽  
pp. 11074-11077
Author(s):  
Zhe Feng ◽  
Rui-Juan Zhao ◽  
Ze-Hua Lu ◽  
Li-ping Jia ◽  
Rong-Na Ma ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Dna Immobilization ◽  
Quenching Effect ◽  
Dual Signal Amplification

A DNA immobilization-free electrochemiluminescence aptasensor was developed for the detection of 8-hydroxy-2′-deoxygunosine based on the diffusion mediated electrochemiluminescence quenching effect and dual signal amplification strategies.

Self-assembled poly-HRP dual signal amplification strategy for high-sensitive detection of circulating miR-142-3p in human serum

2019 ◽  
Vol 279 ◽  
pp. 440-446 ◽  
Author(s):  
Leiliang He ◽  
Lihua Ding ◽  
Songcheng Yu ◽  
Fei Yu ◽  
Yongmei Tian ◽  
...  
Keyword(s):  
Human Serum ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Self Assembled ◽  
Amplification Strategy ◽  
Dual Signal Amplification

scholarly journals Highly Sensitive Detection of PCV2 Based on Tyramide Signals and GNPL Amplification

Molecules ◽  
2019 ◽  
Vol 24 (23) ◽  
pp. 4364
Author(s):  
Shouping Zhang ◽  
Bin Hu ◽  
Xiaojing Xia ◽  
Yanzhao Xu ◽  
Bolin Hang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Secondary Infection ◽  
Porcine Circovirus Type ◽  
Sensitive Detection ◽  
Detection Methods ◽  
Porcine Circovirus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Binding Ability ◽  
Strong Binding ◽  
Dual Signal Amplification

The frequent emergence of secondary infection and immunosuppression after porcine circovirus type 2 (PCV2) infection highlights the need to develop sensitive detection methods. A dual-signal amplification enzyme-linked immunosorbent assay (ELISA) based on a microplate coated with gold nanoparticle layers (GNPL) and tyramide signal amplification (TSA) was established. Results confirmed that the microplates coated with GNPL have a strong binding ability to the antibody without affecting the biological activity of the antibody. The microplates coated with GNPL have strong binding ability to the antibody, and the amplification of the tyramide signal is combined to further improve the sensitivity of PCV2. The PCV2 antibody does not crossreact with other viruses, demonstrating that the method has good specificity. A dual-signal amplification strategy is developed using microplates modified with GNPL and TSA to sensitively detect PCV2.

G-quadruplex DNAzyme-based chemiluminescence biosensing platform based on dual signal amplification for label-free and sensitive detection of protein

2016 ◽  
Vol 79 ◽  
pp. 29-33 ◽  
Author(s):  
Pei Zou ◽  
Yaling Liu ◽  
Hongyong Wang ◽  
Jun Wu ◽  
Feifan Zhu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Label Free ◽  
G Quadruplex ◽  
Dual Signal Amplification

A highly sensitive photoelectrochemical VEGF165 biosensor with a dual signal amplification strategy by using AgVO3 as a photoactive material

2019 ◽  
Vol 55 (56) ◽  
pp. 8076-8078 ◽  
Author(s):  
Huimei Da ◽  
Yaling Liu ◽  
Mengjie Li ◽  
Ruo Yuan ◽  
Hongyan Liu ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Signal Amplification ◽  
Sensitive Detection ◽  
Vascular Endothelial ◽  
Background Signal ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification ◽  
Endothelial Growth Factor

We developed a novel “signal-on” photoelectrochemical (PEC) aptasensor with a near-zero background signal by using AgVO3 as a single photoactive material for the sensitive detection of vascular endothelial growth factor (VEGF165).

A dual signal amplification-assisted DNAzyme biosensor for ultrasensitive detection of Argonaute 2 activity

2018 ◽  
Vol 54 (97) ◽  
pp. 13678-13681 ◽  
Author(s):  
Dandan Zhang ◽  
Fei Ma ◽  
Junhong Leng ◽  
Chun-yang Zhang
Keyword(s):  
Signal Amplification ◽  
Sensitive Detection ◽  
Ultrasensitive Detection ◽  
Argonaute 2 ◽  
Dual Signal Amplification

We develop a new dual-signal amplification-assisted DNAzyme biosensor for sensitive detection of Argonaute 2 (Ago2) activity.

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