scholarly journals Antimicrobial photodynamic therapy fighting polymicrobial infections – a journey from in vitro to in vivo

2020 ◽  
Vol 19 (10) ◽  
pp. 1332-1343
Author(s):  
Lisa Karner ◽  
Susanne Drechsler ◽  
Magdalena Metzger ◽  
Ara Hacobian ◽  
Barbara Schädl ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Bacterial Suspension ◽  
Antimicrobial Photodynamic Therapy ◽  
Led Light ◽  
Wound Model ◽  
Red Led ◽  
Polymicrobial Infections

The batericidal effects of antimicrobial photodynamic therapy (aPDT), using methylene blue as a photosensitizer and pulsed red LED light for activation, were tested in various environments in vitro and in a wound model in mice infected with a fecal bacterial suspension.

scholarly journals Antimicrobial Photodynamic Therapy and Dental Plaque: A Systematic Review of the Literature

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
G. C. Santin ◽  
D. S. B. Oliveira ◽  
R. Galo ◽  
M. C. Borsatto ◽  
S. A. M. Corona
Keyword(s):  
Systematic Review ◽  
Photodynamic Therapy ◽  
Data Extraction ◽  
Antimicrobial Photodynamic Therapy ◽  
Review Of The Literature ◽  
Eligibility Criteria ◽  
Dental Biofilm ◽  
Quality Assessments

Background. The aim of this study was to perform a systematic review of the literature on the efficacy of antimicrobial photodynamic therapy (PDTa) on cariogenic dental biofilm.Types of Studies Reviewed. Studiesin vivo,in vitro, andin situwere included. Articles that did not address PDTa, those that did not involve cariogenic biofilm, those that used microorganisms in the plankton phase, and reviews were excluded. Data extraction and quality assessments were performed independently by two raters using a scale.Results. Two hundred forty articles were retrieved; only seventeen of them met the eligibility criteria and were analyzed in the present review. Considerable variability was found regarding the methodologies and application protocols for antimicrobial PDTa. Two articles reported unfavorable results.Practical Implications. The present systematic review does not allow drawing any concrete conclusions regarding the efficacy of antimicrobial PDTa, although this method seems to be a promising option.

Antimicrobial photodynamic therapy in chronic osteomyelitis induced by Staphylococcus aureus: An in vitro and in vivo study

2012 ◽  
Author(s):  
João Alves dos Reis Júnior ◽  
Patrícia Nascimento de Assis ◽  
Garde^nia Matos Paraguassú ◽  
Isabele Cardoso Vieira de de Castro ◽  
Renan Ferreira Trindade ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
Chronic Osteomyelitis ◽  
In Vivo Study ◽  
Antimicrobial Photodynamic Therapy

scholarly journals Antimicrobial Photodynamic Therapy in the Control of Pseudomonas syringae pv. actinidiae Transmission by Kiwifruit Pollen

2020 ◽  
Vol 8 (7) ◽  
pp. 1022
Author(s):  
Margarida M. Lopes ◽  
Maria Bartolomeu ◽  
Ana T. P. C. Gomes ◽  
Etelvina Figueira ◽  
Ricardo Pinto ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Pseudomonas Syringae ◽  
Pollen Germination ◽  
Ex Vivo ◽  
Irradiation Time ◽  
Antimicrobial Photodynamic Therapy ◽  
Bacterial Reduction ◽  
Promising Alternative

Pseudomonas syringae pv. actinidiae (Psa) is a phytopathogen responsible for bacterial canker in kiwifruit plants and can be disseminated through pollen. This study aimed to evaluate the effectiveness of antimicrobial photodynamic therapy (aPDT) in the inactivation of Psa on kiwifruit pollen using New Methylene Blue (NMB) and Methylene Blue (MB) in the presence/absence of potassium iodide (KI). Pollen germination assays were also performed to evaluate if it was affected by aPDT. Higher reduction of Psa was achieved using NMB (5.0 μM) combined with KI (100 mM) in vitro (ca. 8 log CFU mL−1 after 90 min of irradiation), while NMB alone promoted a lower reduction (3.7 log CFU mL−1). The most efficient NMB concentration with KI was used to study the photodynamic efficiency of MB (5.0 μM). MB with KI photo-inactivated Psa more efficiently than NMB, causing the same bacterial reduction (ca. 8 log CFU mL−1) in half the irradiation time (45 min). Therefore, MB was selected for the subsequent ex vivo aPDT assays in pollen. Almost all the Psa cells added artificially to the pollen (3.2 log CFU mL−1) were photo-inactivated (3.1 log CFU mL−1), whereas aPDT had a low effect on pollen natural microorganisms. When KI was added, a significant increase in aPDT effectiveness was observed (4.5 log CFU mL−1). No negative effects were observed in the pollen germination after aPDT. The results show aPDT is an effective and safe method to Psa inactivation on kiwifruit pollen, and MB use is a promising alternative in the control of Psa transmission.

scholarly journals One-Pot Microwave-Assisted Synthesis of Carbon Dots and in vivo and in vitro Antimicrobial Photodynamic Applications

2021 ◽  
Vol 12 ◽  
Author(s):  
María Paulina Romero ◽  
Fernanda Alves ◽  
Mirian Denise Stringasci ◽  
Hilde Harb Buzzá ◽  
Heloísa Ciol ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Carbon Dots ◽  
Microwave Assisted Synthesis ◽  
One Pot ◽  
Blue Led ◽  
Led Light ◽  
Microwave Assisted

Graphical AbstractBrief description of the procedures carried out in this study. In vivo and in vitro antibacterial photodynamic therapy (aPDT) studies, where aPDT mediated by C-DOTS and blue LED light against S. aureus was evaluated.

Antimicrobial photodynamic therapy on Streptococcus mutans is altered by glucose in the presence of methylene blue and red LED

2017 ◽  
Vol 19 ◽  
pp. 1-4 ◽  
Author(s):  
Cintia R. Lima Leal ◽  
Letícia H. Alvarenga ◽  
Tamires Oliveira-Silva ◽  
Ilka T. Kato ◽  
Bianca Godoy-Miranda ◽  
...  
Keyword(s):  
Methylene Blue ◽  
Photodynamic Therapy ◽  
Streptococcus Mutans ◽  
Antimicrobial Photodynamic Therapy ◽  
Red Led

scholarly journals Antimicrobial photodynamic therapy with fulleropyrrolidine: photoinactivation mechanism of Staphylococcus aureus, in vitro and in vivo studies

2015 ◽  
Vol 99 (9) ◽  
pp. 4031-4043 ◽  
Author(s):  
Mariusz Grinholc ◽  
Joanna Nakonieczna ◽  
Grzegorz Fila ◽  
Aleksandra Taraszkiewicz ◽  
Anna Kawiak ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Photodynamic Therapy ◽  
In Vivo Studies ◽  
Antimicrobial Photodynamic Therapy
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