scholarly journals Posttranscriptional site-directed spin labeling of large RNAs with an unnatural base pair system under non-denaturing conditions

2020 ◽  
Vol 11 (35) ◽  
pp. 9655-9664
Author(s):  
Yan Wang ◽  
Venkatesan Kathiresan ◽  
Yaoyi Chen ◽  
Yanping Hu ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Epr Spectroscopy ◽  
Base Pair ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic

Site-directed spin labeling (SDSL) of large RNAs for electron paramagnetic resonance (EPR) spectroscopy has remained challenging to date.

scholarly journals Site-Directed Spin Labeling EPR for Studying Membrane Proteins

2018 ◽  
Vol 2018 ◽  
pp. 1-13 ◽  
Author(s):  
Indra D. Sahu ◽  
Gary A. Lorigan
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Proteins ◽  
Epr Spectroscopy ◽  
Dynamic Properties ◽  
Biological Systems ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Living Organisms ◽  
Electron Paramagnetic

Site-directed spin labeling (SDSL) in combination with electron paramagnetic resonance (EPR) spectroscopy is a rapidly expanding powerful biophysical technique to study the structural and dynamic properties of membrane proteins in a native environment. Membrane proteins are responsible for performing important functions in a wide variety of complicated biological systems that are responsible for the survival of living organisms. In this review, a brief introduction of the most popular SDSL EPR techniques and illustrations of recent applications for studying pertinent structural and dynamic properties on membrane proteins will be discussed.

Localization of metal ions in biomolecules by means of pulsed dipolar EPR spectroscopy

2021 ◽  
Author(s):  
Dinar Abdullin ◽  
Olav Schiemann
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Metal Ions ◽  
Epr Spectroscopy ◽  
Electron Paramagnetic Resonance Spectroscopy ◽  
Spin Labeling ◽  
Resonance Spectroscopy ◽  
Paramagnetic Resonance ◽  
Paramagnetic Metal ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic

A method is introduced in which paramagnetic metal ions are localized by means of trilateration using a combination of site-directed spin labeling and pulsed dipolar electron paramagnetic resonance spectroscopy.

scholarly journals Characterization of the Linker 2 Region in Human Vimentin Using Site-Directed Spin Labeling and Electron Paramagnetic Resonance†

Biochemistry ◽  
2006 ◽  
Vol 45 (39) ◽  
pp. 11737-11743 ◽  
Author(s):  
John F. Hess ◽  
Madhu S. Budamagunta ◽  
Rebecca L. Shipman ◽  
Paul G. FitzGerald ◽  
John C. Voss
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic

scholarly journals Site-directed Spin Labeling and Electron Paramagnetic Resonance Determination of Vimentin Head Domain Structure

2010 ◽  
Vol 285 (20) ◽  
pp. 15278-15285 ◽  
Author(s):  
Atya Aziz ◽  
John F. Hess ◽  
Madhu S. Budamagunta ◽  
John C. Voss ◽  
Paul G. FitzGerald
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Domain Structure ◽  
Spin Labeling ◽  
Paramagnetic Resonance ◽  
Head Domain ◽  
Site Directed Spin Labeling ◽  
Electron Paramagnetic
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