Enzymatically degradable, starch-based layer-by-layer films: application to cytocompatible single-cell nanoencapsulation

Soft Matter ◽  
2020 ◽  
Vol 16 (26) ◽  
pp. 6063-6071 ◽  
Author(s):  
Hee Chul Moon ◽  
Sol Han ◽  
João Borges ◽  
Tamagno Pesqueira ◽  
Hyunwoo Choi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Controlled Release ◽  
Single Cell ◽  
Enzymatic Degradation ◽  
Layer By Layer

Starch-based layer-by-layer (LbL) nanofilms are formed and enzymatically degraded on individual Saccharomyces cerevisiae in a highly cytocompatible fashion. Their enzymatic degradation by α-amylase is also exploited for the controlled release of DNA.

scholarly journals Layer-by-layer Thin Films and Microcapsules for Biosensors and Controlled Release

2012 ◽  
Vol 28 (10) ◽  
pp. 929-938 ◽  
Author(s):  
Katsuhiko SATO ◽  
Shigehiro TAKAHASHI ◽  
Jun-ichi ANZAI
Keyword(s):  
Thin Films ◽  
Controlled Release ◽  
Layer By Layer

Pectin-Chitosan Multilayer Coated Microbeads of Diclofenac Sodium Prepared by Layer-by-Layer Technique

2014 ◽  
Vol 1060 ◽  
pp. 45-49
Author(s):  
Kamonrak Cheewatanakornkool ◽  
Pornsak Sriamornsak
Keyword(s):  
Controlled Release ◽  
Drug Release ◽  
Diclofenac Sodium ◽  
Gastric Fluid ◽  
Layer By Layer ◽  
Release Behavior ◽  
Freeze Dried ◽  
Layer Technique ◽  
Calcium Pectinate ◽  
Layer By Layer Technique

The main objective of this study was to fabricate biopolymer-based microbeads, providing enteric properties and controlled release of diclofenac sodium, using layer-by-layer technique. The calcium pectinate microbeads have been designed and coated with chitosan and pectin multilayers. Drug release was performed in simulate gastric fluid (pH 1.2) for 2 hours, followed by pH 6.8 buffer for 8 hours. The effects of chitosan concentration, number of layer and drying technique on drug release were investigated. The results showed that the calcium pectinate microbeads could be simply prepared by ionotropic gelation and then coated with chitosan and pectin solutions using layer-by-layer procedure. The diameter of the microbeads ranged from 800 to 1000 μm for air-dried samples and from 1 to 2 mm for freeze-dried samples. The freeze-dried microbeads had a rough surface and many pores inside, as observed by SEM. The microbeads coated with 4% chitosan/4% pectin revealed a slower drug release than those coated with 1% chitosan/4% pectin and demonstrated a controlled release pattern. Moreover, different drying techniques and numbers of layer also influenced drug release behavior of the prepared microbeads.

Multilayer composite beads constructed via layer-by-layer self-assembly for lysozyme controlled release

RSC Advances ◽  
2014 ◽  
Vol 4 (46) ◽  
pp. 24369-24376 ◽  
Author(s):  
Jiemin Zhao ◽  
Xiaoping Wang ◽  
Yanshen Kuang ◽  
Yufeng Zhang ◽  
Xiaowen Shi ◽  
...  
Keyword(s):  
Controlled Release ◽  
Self Assembly ◽  
Cross Linking ◽  
Layer By Layer ◽  
Multilayer Composite ◽  
Composite Beads ◽  
Assembly Technique ◽  
Positively Charged

Alginate (ALG)–lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG–LZ beads via a layer-by-layer (LBL) self-assembly technique.

Microcapsules for controlled release fabricated via layer-by-layer self-assembly of polyelectrolytes

2008 ◽  
Vol 3 (2) ◽  
pp. 133-145 ◽  
Author(s):  
Chaoyang Wang ◽  
Shiqu Ye ◽  
Qilong Sun ◽  
Chengyi He ◽  
Weihua Ye ◽  
...  
Keyword(s):  
Controlled Release ◽  
Self Assembly ◽  
Layer By Layer

scholarly journals Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level

The Analyst ◽  
2014 ◽  
Vol 139 (22) ◽  
pp. 5709-5717 ◽  
Author(s):  
A. Mareike Schmidt ◽  
Stephan R. Fagerer ◽  
Konstantins Jefimovs ◽  
Florian Buettner ◽  
Christian Marro ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Single Cell ◽  
Single Cell Level ◽  
Cell Heterogeneity ◽  
Cell Level ◽  
Cell Sensitivity

Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity.

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