Rapid isolation of extracellular vesicles from diverse biofluid matrices via capillary-channeled polymer fiber solid-phase extraction micropipette tips

The Analyst ◽  
2021 ◽  
Author(s):  
Kaylan K. Jackson ◽  
Rhonda R. Powell ◽  
Terri F. Bruce ◽  
R. Kenneth Marcus
Keyword(s):  
Solid Phase Extraction ◽  
Extracellular Vesicles ◽  
Solid Phase ◽  
Phase Extraction ◽  
Polymer Fiber ◽  
Multiple Methods ◽  
Rapid Isolation ◽  
High Yields

C-CP fiber spin-down tips are employed on a table-top centrifuge to isolate extracellular vesicles from diverse biofluids in less than 15 min. The EVs are isolated in high yields and purity, with the recoveries verified across multiple methods.

Rapid Isolation of Red Wine Polymeric Polyphenols by Solid-Phase Extraction

2008 ◽  
Vol 56 (8) ◽  
pp. 2571-2580 ◽  
Author(s):  
David W. Jeffery ◽  
Meagan D. Mercurio ◽  
Markus J. Herderich ◽  
Yoji Hayasaka ◽  
Paul A. Smith
Keyword(s):  
Solid Phase Extraction ◽  
Red Wine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Rapid Isolation

scholarly journals Synthesis and Characterization of TiO2Modified with Polystyrene and Poly(3-chloro-2-hydroxypropyl Methacrylate) as Adsorbents for the Solid Phase Extraction of Organophosphorus Pesticides

2016 ◽  
Vol 2016 ◽  
pp. 1-11 ◽  
Author(s):  
Enrique Alejo-Molina ◽  
A. Rafael Vilchis-Néstor ◽  
David Muñoz-Rodríguez ◽  
Cristian Carrera-Figueiras
Keyword(s):  
Solid Phase Extraction ◽  
High Performance ◽  
Solid Phase ◽  
Organophosphorus Pesticides ◽  
Sol Gel ◽  
Phase Extraction ◽  
Hydroxypropyl Methacrylate ◽  
Inorganic Matter ◽  
Pore Size Distributions ◽  
High Yields

Novel hybrid TiO2particles were developed and assessed as an adsorbent for solid phase extraction (SPE) of organophosphorus pesticides (fensulfothion, parathion methyl, coumaphos, and diazinon) from spiked water. The sol-gel method was used to synthesize TiO2particles, which were coated with free-radical polystyrene (PS) and poly(3-chloro-2-hydroxypropyl methacrylate) (PClHPMA) polymers. Particle structures were determined via Fourier transform infrared spectroscopy to confirm that the polymers were successfully anchored to the TiO2particles. Thermogravimetric analysis was conducted to determine organic and inorganic matter in TiO2-PS and TiO2-PClHPMA particles showing results of 20 : 80 wt/wt% and 23 : 77 wt/wt%, respectively. SEM-EDS and X-ray diffraction test were conducted to determine the morphology and semielemental composition of the particles showing amorphous characteristics. By observing the contact angle, particles coated with PClHPMA were determined to be more hydrophilic than TiO2-PS particles. The pore size distributions obtained from the N2adsorption-desorption isotherms were 0.150 and 0.168 cm3g−1. The specific surface area (BET) was 239.9 m2g−1for TiO2-PS and 225.7 m2g−1for TiO2-PClHPMA. The synthesized particles showed relatively high yields of adsorption in SPE. The pesticide recoveries obtained by high performance liquid chromatography ranged from 6 to 26% for TiO2-PClHPMA and 44 to 92% for TiO2-PS.

Sign in / Sign up

Export Citation Format

Share Document