The dynamicity of light-up aptamers in one-pot in vitro diagnostic assays

The Analyst ◽  
2022 ◽  
Author(s):  
Marimuthu Citartan
Keyword(s):  
Diagnostic Assay ◽  
One Pot ◽  
Direct Modulation ◽  
Diagnostic Assays ◽  
In Vitro Diagnostic ◽  
Targeted Detection

The direct modulation of a light-up aptamer that engenders an analyte-specific aptamer-light-up aptamer chimera is readily applicable in any diagnostic assay for a targeted detection.

PERFORMANCE OF COMMERCIALLY AVAILABLE HIV IN VITRO DIAGNOSTIC ASSAYS: SYSTEMATIC REVIEW AND META-ANALYSIS

2021 ◽  
pp. 105047
Author(s):  
Mihir Bhatta ◽  
Santanu Banerjee ◽  
Srijita Nandi ◽  
Shanta Dutta ◽  
Dr. Malay Kumar Saha
Keyword(s):  
Systematic Review ◽  
Meta Analysis ◽  
Diagnostic Assays ◽  
In Vitro Diagnostic

scholarly journals Clinical Evaluation and Utilization of Multiple Molecular In Vitro Diagnostic Assays for the Detection of SARS-CoV-2

2020 ◽  
Vol 154 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Kendall Cradic ◽  
Marie Lockhart ◽  
Patrick Ozbolt ◽  
Lisa Fatica ◽  
Lorie Landon ◽  
...  
Keyword(s):  
Clinical Performance ◽  
Nasopharyngeal Swab ◽  
Separate Analysis ◽  
Assay Sensitivity ◽  
Limits Of Detection ◽  
Diagnostic Assays ◽  
Percent Agreement ◽  
In Vitro Diagnostic ◽  
Roche Cobas

Abstract Objectives To evaluate the clinical performance of 3 molecular assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods We used 184 nasopharyngeal swab specimens to compare Abbott ID NOW COVID-19 (Abbott ID NOW), DiaSorin Molecular Simplexa COVID-19 Direct (DiaSorin Simplexa), and Roche cobas 6800 SARS-CoV-2 (Roche cobas) assays. In a separate analysis, 3 specimens (nasopharyngeal, oropharyngeal, and nasal) were collected from 182 unique patients presenting to the emergency department with suspicion of coronavirus disease 2019 and were tested utilizing Abbott ID NOW. To further characterize each assay, relative limits of detection were evaluated utilizing positive nasopharyngeal patient samples. Results The positive percent agreement was 91% (95% confidence interval [CI], 0.76-0.97) for Abbott ID NOW and 100% (95% CI, 0.90-1.00) for DiaSorin Simplexa and Roche cobas. The negative percent agreement was 100% (95% CI, 0.98-1.00) for all 3 assays. All swab types tested with the Abbott assay produced concordant results. Polymerase chain reaction assays had approximately 10 to 100 times lower limits of detection than Abbott ID NOW. Conclusions Based on these evaluations, a multiplatform testing approach is proposed, depending on patient population and assay sensitivity, to address testing needs during a public health emergency.

89 Comparative Quantification of Plasma Progesterone Through Radioimmumoassay and Enzyme-Linked Fluorescent Assay Techniques in Cattle

2018 ◽  
Vol 30 (1) ◽  
pp. 184
Author(s):  
J. M. R. Pérsico ◽  
C. Bianchi ◽  
C. Tapia ◽  
S. Raggio ◽  
I. A. Marchetti
Keyword(s):  
Predictive Value ◽  
Prostaglandin F2α ◽  
Standard Technique ◽  
Diagnostic Assay ◽  
Becton Dickinson ◽  
Bovine Reproduction ◽  
Coefficients Of Variation ◽  
In Vitro Diagnostic ◽  
Group B

Progesterone (P4) is an important component of oestrous cyclicity and is critical to fertility. A concentration >1 ng mL−1 reflects the function of the corpus luteum (CL) and is considered indicative of a cyclic cow. Recent publications have shown that P4 at the onset of synchronization programs is critical to pregnancy outcomes in primiparous cows (Stevenson et al. 2015 J. Anim. Sci. 93, 2111-2123) and cows with P4 <5 ng mL−1 on Day 14 could predict pregnancy loss (Kenyon et al. 2013 Anim. Reprod. Sci. 136, 223-230). Currently, the gold standard technique to quantify P4 is radioimmunoassay (RIA). However, new techniques are emerging. The objective of this study was to evaluate a new commercial in vitro diagnostic assay to quantify P4 based on enzyme immunoassay by competition with detection of final fluorescence (enzyme-linked fluorescence assay, ELFA). A total of 30 cows were synchronized on Day 0 with an intravaginal device (IVD) containing 500 mg of P4 (Cronipres, Biogénesis Bagó, Argentina). On Day 7 and Day 8 all cows received 150 mg of prostaglandin F2α (Enzaprost, Biogénesis Bagó). All IVD were removed on Day 8. A total of 95 blood samples were taken at Days 0, 9, 9.5, and 10 using BD Vacutainer (Becton-Dickinson, Franklin Lakes, NJ, USA) with sodium heparin by jugular venipuncture and centrifuged at 3000 × g for 30 min for plasma separation, which was frozen at –20°C until analysis. Samples were measured in duplicate by IM1188-Progesterone-RIA (Beckman Coulter, Brea, CA, USA) and VIDAS-PRG-ELFA (Biomerieux, Marcy l’Étoile, France). Concentrations of P4 obtained by RIA were classified in 2 groups: (A) P4 <1 ng mL−1, and (B) P4 ≥1 ng mL−1 and matched with P4 concentrations obtained by ELFA. Kappa (κ) test was used to determine agreement between both techniques, intra-assay coefficients of variation was determined for RIA and ELFA; and sensitivity (SE), specificity (SP), positive predictive value (PPV), and negative predictive value (NPV) were determined for ELFA. There was very good agreement between the RIA and ELFA techniques, κ = 0.95, to determine P4 concentrations for group A (62 and 62 samples, respectively) and group B (33 and 33 samples, respectively). The intra-assay coefficients of variation were 5% (RIA) and 2.9% (ELFA). Values for SE = 0.97, SP = 0.98, PPV = 0.97, and NPV = 0.98 were obtained for ELFA. We were able to quantify P4 in bovine plasma in all samples using the ELFA technique with similar reliability to the RIA technique. Only 2 samples (2.1%) differed in their concentrations and clinical interpretation. There was a slight discrepancy between the results found for both techniques with an excellent SE and SP in ELFA compared with RIA. Based on the analytical results, we believe that this in vitro diagnostic assay developed for use with an autoanalyzer could be useful for routine bovine reproduction programs. Further studies should be carried out to strengthen these conclusions.

Comparison of candidate vCJD in vitro diagnostic assays using identical sample sets

Vox Sanguinis ◽  
2011 ◽  
Vol 102 (2) ◽  
pp. 100-109 ◽  
Author(s):  
J. K. Cooper ◽  
K. Ladhani ◽  
P. Minor
Keyword(s):  
Diagnostic Assays ◽  
In Vitro Diagnostic
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