A label-free fluorescent biosensor based on catalyzed hairpin assembly for HIV DNA and lead detection

2021 ◽  
Author(s):  
Yu Xiong ◽  
Jianyuan Dai ◽  
Yujiao Zhang ◽  
Cuisong Zhou ◽  
Dan Xiao ◽  
...  
Keyword(s):  
Label Free ◽  
Fluorescent Signal ◽  
Fluorescent Biosensor ◽  
Hiv Dna ◽  
Lead Detection ◽  
G Quadruplex ◽  
Hairpin Probes ◽  
Catalyzed Hairpin Assembly

Herein, a label-free fluorescent signal amplified system based on catalyzed hairpin assembly (CHA) was reported. In this system, two hairpin probes, H1 and H2, were well-designed in which G-quadruplex sequences...

Cationic-perylene-G-quadruplex complex based fluorescent biosensor for label-free detection of Pb 2+

Talanta ◽  
2016 ◽  
Vol 149 ◽  
pp. 98-102 ◽  
Author(s):  
Xu-Hua Zhao ◽  
Liang Gong ◽  
Yuan Wu ◽  
Xiao-Bing Zhang ◽  
Jun Xie
Keyword(s):  
Label Free ◽  
Fluorescent Biosensor ◽  
Label Free Detection ◽  
G Quadruplex

An enzyme-free and label-free fluorescent biosensor for small molecules by G-quadruplex based hybridization chain reaction

Talanta ◽  
2015 ◽  
Vol 138 ◽  
pp. 15-19 ◽  
Author(s):  
Qingai Chen ◽  
Qingquan Guo ◽  
Ying Chen ◽  
Jie Pang ◽  
Fengfu Fu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Fluorescent Biosensor ◽  
G Quadruplex

A G-quadruplex based label-free fluorescent biosensor for lead ion

2012 ◽  
Vol 35 (1) ◽  
pp. 123-127 ◽  
Author(s):  
Liangqia Guo ◽  
Dandan Nie ◽  
Chunyan Qiu ◽  
Qishan Zheng ◽  
Haiyan Wu ◽  
...  
Keyword(s):  
Lead Ion ◽  
Label Free ◽  
Fluorescent Biosensor ◽  
G Quadruplex

A label-free fluorescent aptasensor based on HCR and G-quadruplex DNAzymes for the detection of prostate-specific antigen

The Analyst ◽  
2021 ◽  
Author(s):  
Ruirui Zhao ◽  
Lu Zhao ◽  
Haidi Feng ◽  
Xiaoliang Chen ◽  
Huilin Zhang ◽  
...  
Keyword(s):  
Prostate Specific Antigen ◽  
Specific Antigen ◽  
Label Free ◽  
Fluorescence Sensing ◽  
Sensing Platforms ◽  
G Quadruplex ◽  
Fluorescent Aptasensor

Fluorescence sensing platforms based on HCR and G-quadruplex DNAzyme amplification strategies for the detection of prostate-specific antigen.

scholarly journals A Turn-ON fluorometric biosensor based on ssDNA immobilized with a metal phenolic nanomaterial for the sequential detection of Pb(ii) and epirubicin cancer drug

RSC Advances ◽  
2021 ◽  
Vol 11 (20) ◽  
pp. 12361-12373
Author(s):  
A. Arunjegan ◽  
P. Rajaji ◽  
S. Sivanesan ◽  
P. Panneerselvam
Keyword(s):  
Acridine Orange ◽  
Cancer Drug ◽  
Sequential Detection ◽  
Label Free ◽  
Fluorescent Biosensor ◽  
Turn On

In this paper, we propose a fluorescent biosensor for the sequential detection of Pb2+ ions and the cancer drug epirubicin (Epn) using the interactions between label-free guanine-rich ssDNA (LFGr-ssDNA), acridine orange (AO), and a metal–phenolic nanomaterial.

A rapid label- and enzyme-free G-quadruplex-based fluorescence strategy for highly-sensitive detection of HIV DNA

The Analyst ◽  
2020 ◽  
Vol 145 (1) ◽  
pp. 206-212 ◽  
Author(s):  
Feng Zhang ◽  
Ling Xiang ◽  
Xianghui Xiao ◽  
Xiaoming Chen ◽  
Chunyan Chen ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Sensitive Detection ◽  
Hiv Dna ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Highly Sensitive Detection

Because rapid and selective methods for HIV detection are urgently needed, herein, a simple label- and enzyme-free strategy is constructed for fluorescence detection of HIV DNA.

A label-free and enzyme-free signal amplification strategy for a sensitive RNase H activity assay

Nanoscale ◽  
2017 ◽  
Vol 9 (42) ◽  
pp. 16149-16153 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hyowon Jang ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Signal Amplification ◽  
Rnase H ◽  
Label Free ◽  
Activity Assay ◽  
G Quadruplex ◽  
Catalytic Hairpin Assembly ◽  
Amplification Strategy

A target-triggered catalytic hairpin assembly with a G-quadruplex specific fluorescent binder, NMM, is employed to develop a novel and sensitive RNase H activity assay.

A Highly Sensitive and Label-Free Microbead-Based ‘Turn-On’ Colorimetric Sensor for the Detection of Mercury(II) in Urine Using a Peroxidase-Like Split G-Quadruplex–Hemin DNAzyme

2018 ◽  
Vol 71 (12) ◽  
pp. 945
Author(s):  
Xin Fu ◽  
He Zhang ◽  
Jie Zhang ◽  
Shi-Tong Wen ◽  
Xing-Cheng Deng
Keyword(s):  
Standard Deviation ◽  
Base Pair ◽  
Limit Of Detection ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Relative Standard ◽  
G Quadruplex ◽  
Catalytically Active ◽  
Partial Cdna

A highly sensitive and label-free microbead-based ‘turn-on’ assay was developed for the detection of Hg2+ in urine based on the Hg2+-mediated formation of intermolecular split G-quadruplex–hemin DNAzymes. In the presence of Hg2+, T–T mismatches between the two partial cDNA strands were stabilized by a T–Hg2+–T base pair, and can cause the G-rich sequences of the two oligonucleotides to associate to form a split G-quadruplex which is able to bind hemin to form the catalytically active G-quadruplex–hemin DNAzyme. This microbead-based ‘turn-on’ process allows the detection of Hg2+ in urine samples at concentrations as low as 0.5 pM. The relative standard deviation and recovery are 1.2–3.9 and 98.7–103.2%, respectively. The remarkable sensitivity for Hg2+ is mainly attributed to the enhanced mass transport ability that is inherent in homogeneous microbead-based assays. Compared with previous developments of intermolecular split G-quardruplex–hemin DNAzymes for the homogeneous detection of Hg2+ (the limit of detection was 19nM), a signal enhancement of ~1000 times is obtained when such an assay is performed on the surface of microbeads.

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