A guide-tag system controlling client enrichment into Y15 peptide-based granules for an in-cell protein recruitment assay

2021 ◽  
Author(s):  
Takayuki Miki ◽  
Masahiro Hashimoto ◽  
Taichi Nakai ◽  
Hisakazu Mihara
Keyword(s):  
Protein Interactions ◽  
Living Cells ◽  
The Self ◽  
Cell Protein ◽  
Protein Protein Interactions ◽  
Self Assembling ◽  
Client Proteins ◽  
Protein Recruitment ◽  
Peptide Tag

A series of guide-tags that can control the enrichment of client proteins into artificial scaffolds constituted by the self-assembling Y15 peptide tag facilitates the analysis of protein–protein interactions in living cells.

scholarly journals A Flow Cytometry-Based FRET Assay to Identify and Analyse Protein-Protein Interactions in Living Cells

PLoS ONE ◽  
2010 ◽  
Vol 5 (2) ◽  
pp. e9344 ◽  
Author(s):  
Carina Banning ◽  
Jörg Votteler ◽  
Dirk Hoffmann ◽  
Herwig Koppensteiner ◽  
Martin Warmer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Protein Interactions ◽  
Living Cells ◽  
Protein Protein Interactions

Femtosecond UV-laser pulses to unveil protein–protein interactions in living cells

2015 ◽  
Vol 73 (3) ◽  
pp. 637-648 ◽  
Author(s):  
Francesco Itri ◽  
Daria M. Monti ◽  
Bartolomeo Della Ventura ◽  
Roberto Vinciguerra ◽  
Marco Chino ◽  
...  
Keyword(s):  
Protein Interactions ◽  
Laser Pulses ◽  
Living Cells ◽  
Uv Laser ◽  
Protein Protein Interactions

qPCA: a scalable assay to measure the perturbation of protein–protein interactions in living cells

2013 ◽  
Vol 9 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Luca Freschi ◽  
Francisco Torres-Quiroz ◽  
Alexandre K. Dubé ◽  
Christian R. Landry
Keyword(s):  
Protein Interactions ◽  
Living Cells ◽  
Protein Protein Interactions
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