scholarly journals A sensitive platform for DNA detection based on organic electrochemical transistor and nucleic acid self-assembly signal amplification

RSC Advances ◽  
2021 ◽  
Vol 11 (60) ◽  
pp. 37917-37922
Author(s):  
Chaohui Chen ◽  
Qingyuan Song ◽  
Wangting Lu ◽  
Zhengtao Zhang ◽  
Yanhua Yu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Self Assembly ◽  
Signal Amplification ◽  
Dna Detection ◽  
New Method ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Electrochemical Transistor ◽  
First Time

A new method has been developed for DNA detection by integrating hybridization chain reaction signal amplification with organic electrochemical transistor device for the first time.

scholarly journals Sensitive DNA detection using cascade amplification strategy based on conjugated polyelectrolytes and hybridization chain reaction

RSC Advances ◽  
2017 ◽  
Vol 7 (6) ◽  
pp. 3528-3533 ◽  
Author(s):  
Biqing Bao ◽  
Jin Zhu ◽  
Lina Gong ◽  
Jia Chen ◽  
Yanrui Pan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Detection ◽  
Hybridization Chain Reaction ◽  
Molecular Wire ◽  
Conjugated Polyelectrolytes ◽  
Chain Reaction ◽  
Amplification Strategy ◽  
Cascade Amplification

A novel cascade amplification strategy that combines the molecular wire effects of CPEs with the signal amplification capability of the HCR has been developed for sensitive DNA detection.

scholarly journals Elucidation of leak-resistance DNA hybridization chain reaction with universality and extensibility

2020 ◽  
Vol 48 (5) ◽  
pp. 2220-2231 ◽  
Author(s):  
Shaofei Li ◽  
Pan Li ◽  
Meihong Ge ◽  
Hongzhi Wang ◽  
Yizhuang Cheng ◽  
...  
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
Clinical Medicine ◽  
Surface Enhanced Raman Spectroscopy ◽  
Careful Consideration ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Dna Hairpins ◽  
Surface Enhanced Raman ◽  
Thermodynamics And Dynamics

Abstract Hybridization chain reaction (HCR) was a significant discovery for the development of nanoscale materials and devices. One key challenge for HCR is the vulnerability to background leakage in the absence of the initiator. Here, we systematically analyze the sources of leakage and refine leak-resistant rule by using molecular thermodynamics and dynamics, biochemical and biophysical methods. Transient melting of DNA hairpin is revealed to be the underlying cause of leakage and that this can be mitigated through careful consideration of the sequence thermodynamics. The transition threshold of the energy barrier is proposed as a testing benchmark of leak-resistance DNA hairpins. The universal design of DNA hairpins is illustrated by the analysis of hsa-miR-21-5p as biomarker when used in conjunction with surface-enhanced Raman spectroscopy. We further extend the strategy for specific signal amplification of miRNA homologs. Significantly, it possibly provides a practical route to improve the accuracy of DNA self-assembly for signal amplification, and that could facilitate the development of sensors for the sensitive detection of interest molecules in biotechnology and clinical medicine.

A cytometric bead assay for sensitive DNA detection based on enzyme-free signal amplification of hybridization chain reaction

2013 ◽  
Vol 49 ◽  
pp. 380-386 ◽  
Author(s):  
Wei Ren ◽  
Hongmei Liu ◽  
Wenxia Yang ◽  
Yunlong Fan ◽  
Lang Yang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Dna Detection ◽  
Hybridization Chain Reaction ◽  
Chain Reaction

A new method for the in situ assay of α-glucosidase activity and inhibitor screening through an enzyme substrate-mediated DNA hybridization chain reaction

2019 ◽  
Vol 43 (24) ◽  
pp. 9458-9465
Author(s):  
Xiquan Yue ◽  
Lihong Su ◽  
Xu Chen ◽  
Junfeng Liu ◽  
Longpo Zheng ◽  
...  
Keyword(s):  
Small Molecule ◽  
Dna Hybridization ◽  
New Method ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Inhibitor Screening ◽  
Glucosidase Activity ◽  
Enzyme Substrate ◽  
In Situ Assay

The strategy is based on small molecule-mediated hybridization chain reaction.

Silver nanocluster-lightened hybridization chain reaction

RSC Advances ◽  
2016 ◽  
Vol 6 (62) ◽  
pp. 57502-57506 ◽  
Author(s):  
Lin Liu ◽  
Qing Li ◽  
Li-Juan Tang ◽  
Ru-Qin Yu ◽  
Jian-Hui Jiang
Keyword(s):  
Nucleic Acid ◽  
Silver Nanoclusters ◽  
Hybridization Chain Reaction ◽  
Label Free ◽  
Chain Reaction ◽  
Silver Nanocluster ◽  
Turn On ◽  
Nucleic Acid Assays

A hybridization chain reaction (HCR) lightened by DNA-stabilized silver nanoclusters (AgNCs) as a label-free and turn on fluorescence platform for nucleic acid assays.

Lab in a tube: a fast-assembled colorimetric sensor for highly sensitive detection of oligonucleotides based on a hybridization chain reaction

RSC Advances ◽  
2015 ◽  
Vol 5 (55) ◽  
pp. 44714-44721 ◽  
Author(s):  
Siqi Zhang ◽  
Kun Wang ◽  
Zhenyu Li ◽  
Zhongmin Feng ◽  
Ting Sun
Keyword(s):  
Complex Formation ◽  
Dna Sequence ◽  
Self Assembly ◽  
The Self ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Colored Product ◽  
Highly Sensitive Detection

Upon adding THBV, the self-assembly of THBV with H1 allows the rest of the DNA sequence of H1 to accelerate H1–H2 complex formation. The G-quadruplex at the end of the H1–H2 complex could catalyze TMB into a colored product.

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