Optimization of submerged-culture conditions for mycelial growth and exo-biopolymer production by Auricularia polytricha (wood ears fungus) using the methods of uniform design and regression analysis

2003 ◽  
Vol 38 (2) ◽  
pp. 193 ◽  
Author(s):  
Chun-Ping Xu ◽  
Jong-Won Yun
Keyword(s):  
Regression Analysis ◽  
Submerged Culture ◽  
Mycelial Growth ◽  
Uniform Design ◽  
Culture Conditions ◽  
Auricularia Polytricha

Optimization of Culture Conditions on Mycelial Grown in Submerged Culture of Cordyceps militaris

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jian Dong Cui ◽  
Li Qiang Yuan
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Fold Increase ◽  
Culture Conditions ◽  
Statistical Experimental Design ◽  
Fermentation Time ◽  
Original Culture ◽  
Production Values ◽  
Validation Experiment ◽  
Optimal Region

Statistical experimental design strategy (SES) was employed to optimize the culture conditions for the mycelial growth of C. militaris by submerged culture. Using Plackett-Burman design (PBD), liquid volumes in flask and fermentation time were identified as the significant variables that would highly influence mycelial growth, and these variables were subsequently optimized using response surface method (RSM). The steepest ascent method was used to access the optimal region of the culture condition. The results showed that the optimum values of the tested variables were 47.3 mL of liquid volumes in flask and 7.6 days of fermentation time. Under optimized culture conditions, the mycelial production was enhanced from 10.33 to 19.97 g/L. In comparison with that of original culture conditions, 1.9-fold increase was obtained. The validation experiment showed that the experimentally determined production values were in close agreement with the statistically predicted ones.

Optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Paecilomyces tenuipes C240

2003 ◽  
Vol 38 (7) ◽  
pp. 1025-1030 ◽  
Author(s):  
Chun-Ping Xu ◽  
Sang-Woo Kim ◽  
Hye-Jin Hwang ◽  
Jang-Won Choi ◽  
Jong-Won Yun
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Culture Conditions

Effects of Culture Conditions on Mycelial Growth and Luminescence inPanellus Stypticus

Mycologia ◽  
1990 ◽  
Vol 82 (3) ◽  
pp. 295-305 ◽  
Author(s):  
David Bermudes ◽  
Valerie L. Gerlach ◽  
Kenneth H. Nealson
Keyword(s):  
Mycelial Growth ◽  
Culture Conditions

scholarly journals A preliminary strategy for screening the combination of active components based on Bu–Shen–Yi–Qi Decoction for bronchial asthma

2019 ◽  
Vol 02 (03) ◽  
pp. 135-138
Author(s):  
Chen Yan ◽  
Qingli Luo ◽  
Cheng Ma ◽  
Jiaqi Liu ◽  
Jingcheng Dong
Keyword(s):  
Regression Analysis ◽  
Bronchial Asthma ◽  
Molecular Mechanisms ◽  
Uniform Design ◽  
Chinese Herbs ◽  
Chinese Drug ◽  
Active Components ◽  
Control Drug ◽  
Effective Components ◽  
Herba Epimedii

Objective: The anti-asthma herbal medicine Bu–Shen–Yi–Qi Decoction (BSYQ Decoction), a combination of three traditional Chinese medicine herbs developed in our lab, has shown demonstrated efficacy in Ovalbumin (OVA)-induced acute bronchial asthma. However, the obscure substances, multiple potential targets and the unclear molecular mechanisms are obstacles to control drug quality, stability and standardization. Multi-component formulae with a clear pharmacodynamic material and specific mechanism are an innovation worth exploring. They may also partly reserve a potential synergistic and additive effect compared with single components extracted from traditional Chinese herbs. This study was designed to select three standard multi-component formulae of the combination of effective components preliminarily based on four effective components [total flavonoids of Herba Epimedii ([Formula: see text]; Astragalus polysaccharide ([Formula: see text]; Astragaloside ([Formula: see text]; and Catalpol ([Formula: see text]] in BSYQ Decoction. Methods: OVA-induced asthmatic murine models were established. A uniform design was applied to select 10 proportions from four target components, and 3 formulae which showed best effect aimed at IFN-[Formula: see text], IL-4 and IgE in the serum respectively were screened based on stepwise regression analysis. Results: According to the regression analysis, Formula I ([Formula: see text]:[Formula: see text]:[Formula: see text]:6:29), Formula II ([Formula: see text]:[Formula: see text]:[Formula: see text]:8:2) and Formula III ([Formula: see text]:[Formula: see text]:[Formula: see text]:[Formula: see text]:50:8:2) are the three potential proper ratios for the new multi-component formula aimed at serum IFN-[Formula: see text], IL-4 and IgE in OVA-induced asthma mice, respectively. Conclusion: Three multi-component formulae derived from BSYQ Decoction could exert anti-inflammatory effect against OVA-induced asthma, which might provide evidences and lay foundations for further study of standard modern Chinese drug for treating asthma based on BSYQ Decoction.

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