scholarly journals Optimization of Submerged Culture Conditions for Mycelial Growth and Extracellular Polysaccharide Production by Coriolus versiolor

2012 ◽  
Vol 02 (04) ◽  
Author(s):  
Feifei Wang
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Extracellular Polysaccharide ◽  
Culture Conditions ◽  
Polysaccharide Production

Optimization of Culture Conditions on Mycelial Grown in Submerged Culture of Cordyceps militaris

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jian Dong Cui ◽  
Li Qiang Yuan
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Fold Increase ◽  
Culture Conditions ◽  
Statistical Experimental Design ◽  
Fermentation Time ◽  
Original Culture ◽  
Production Values ◽  
Validation Experiment ◽  
Optimal Region

Statistical experimental design strategy (SES) was employed to optimize the culture conditions for the mycelial growth of C. militaris by submerged culture. Using Plackett-Burman design (PBD), liquid volumes in flask and fermentation time were identified as the significant variables that would highly influence mycelial growth, and these variables were subsequently optimized using response surface method (RSM). The steepest ascent method was used to access the optimal region of the culture condition. The results showed that the optimum values of the tested variables were 47.3 mL of liquid volumes in flask and 7.6 days of fermentation time. Under optimized culture conditions, the mycelial production was enhanced from 10.33 to 19.97 g/L. In comparison with that of original culture conditions, 1.9-fold increase was obtained. The validation experiment showed that the experimentally determined production values were in close agreement with the statistically predicted ones.

Optimization of submerged culture conditions for mycelial polysaccharide production in Cordyceps pruinosa

2004 ◽  
Vol 39 (12) ◽  
pp. 2241-2247 ◽  
Author(s):  
Jian Hui Xiao ◽  
Dai Xiong Chen ◽  
Yu Xiao ◽  
Jin Wei Liu ◽  
Zu Lin Liu ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Culture Conditions ◽  
Polysaccharide Production

Optimization of submerged culture conditions for mycelial growth and exo-biopolymer production by Paecilomyces tenuipes C240

2003 ◽  
Vol 38 (7) ◽  
pp. 1025-1030 ◽  
Author(s):  
Chun-Ping Xu ◽  
Sang-Woo Kim ◽  
Hye-Jin Hwang ◽  
Jang-Won Choi ◽  
Jong-Won Yun
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Culture Conditions

scholarly journals Influence of culture conditions on extracellular polysaccharide production and the activities of enzymes involved in the polysaccharide synthesis of Nostoc flagelliforme

RSC Advances ◽  
2017 ◽  
Vol 7 (71) ◽  
pp. 45075-45084 ◽  
Author(s):  
Pei-pei Han ◽  
Shun-yu Yao ◽  
Rong-jun Guo ◽  
Rong-rong Yan ◽  
Yi-kai Wu ◽  
...  
Keyword(s):  
Extracellular Polysaccharide ◽  
Culture Conditions ◽  
Nostoc Flagelliforme ◽  
Polysaccharide Production ◽  
Polysaccharide Synthesis

Important enzymes influencing the production ofNostoc flagelliformeEPS were investigated under different culture conditions.

scholarly journals Effect of Different Carbon Sources on the Extra-cellular Polysaccharide Production by Tremella cinnabarina in Submerged Culture

1970 ◽  
Vol 8 (2) ◽  
pp. 147-152
Author(s):  
Proma Khondkar
Keyword(s):  
Fatty Acids ◽  
Submerged Culture ◽  
Mycelial Growth ◽  
Seed Oil ◽  
Carbon Sources ◽  
Grape Seed ◽  
Plant Oils ◽  
Poor Growth ◽  
Polysaccharide Production ◽  
Grape Seed Oil

The effect of fatty acids, emulsifiers and plant oils were studied with respect to their potential to support the growth and extra-cellular polysaccharide production of Tremella cinnabarina in submerged culture. The extra-cellular polysaccharide (EPS) production and mycelial growth were substantially increased by supplementation with certain fatty acids in the medium. Oleic acid, sorbitan mono-oleate and grape-seed oil significantly enhanced EPS production which were 1.5, 1.6 and 1.3 folds, respectively, in comparison to the control. In case of lipids, the results indicated that the extent of stimulation or inhibition was associated with the types and levels of lipids. However, when lipids were used as sole carbon source, poor growth and lack of EPS production were observed. Key words: T. cinnabarina; extra-cellular polysaccharide; emulsifier; fatty acids DOI: 10.3329/dujps.v8i2.6029 Dhaka Univ. J. Pharm. Sci. 8(2): 147-152, 2009 (December)

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