Enhanced Bioactive Exopolysaccharide Production by Mossy Maze Polypore, Cerrena unicolor (Higher Basidiomycetes) in Submerged Culture Conditions

2015 ◽  
Vol 17 (7) ◽  
pp. 639-648
Author(s):  
Burcu Altinay ◽  
Ayse Betul Karaduman ◽  
Bukay Yenice Gursu ◽  
Mustafa Yamac
Keyword(s):  
Submerged Culture ◽  
Culture Conditions ◽  
Exopolysaccharide Production ◽  
Cerrena Unicolor

Optimization of submerged culture conditions for exopolysaccharide production in Sarcodon aspratus (Berk) S.lto TG-3

2004 ◽  
Vol 20 (7) ◽  
pp. 767-773 ◽  
Author(s):  
Ji Hoon Joo ◽  
Jong Min Lim ◽  
Hyun Oh Kim ◽  
Sang Woo Kim ◽  
Hye Jin Hwang ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Culture Conditions ◽  
Exopolysaccharide Production

Optimization of Culture Conditions on Mycelial Grown in Submerged Culture of Cordyceps militaris

2011 ◽  
Vol 7 (1) ◽  
Author(s):  
Jian Dong Cui ◽  
Li Qiang Yuan
Keyword(s):  
Submerged Culture ◽  
Mycelial Growth ◽  
Fold Increase ◽  
Culture Conditions ◽  
Statistical Experimental Design ◽  
Fermentation Time ◽  
Original Culture ◽  
Production Values ◽  
Validation Experiment ◽  
Optimal Region

Statistical experimental design strategy (SES) was employed to optimize the culture conditions for the mycelial growth of C. militaris by submerged culture. Using Plackett-Burman design (PBD), liquid volumes in flask and fermentation time were identified as the significant variables that would highly influence mycelial growth, and these variables were subsequently optimized using response surface method (RSM). The steepest ascent method was used to access the optimal region of the culture condition. The results showed that the optimum values of the tested variables were 47.3 mL of liquid volumes in flask and 7.6 days of fermentation time. Under optimized culture conditions, the mycelial production was enhanced from 10.33 to 19.97 g/L. In comparison with that of original culture conditions, 1.9-fold increase was obtained. The validation experiment showed that the experimentally determined production values were in close agreement with the statistically predicted ones.

Optimization of submerged culture conditions for mycelial polysaccharide production in Cordyceps pruinosa

2004 ◽  
Vol 39 (12) ◽  
pp. 2241-2247 ◽  
Author(s):  
Jian Hui Xiao ◽  
Dai Xiong Chen ◽  
Yu Xiao ◽  
Jin Wei Liu ◽  
Zu Lin Liu ◽  
...  
Keyword(s):  
Submerged Culture ◽  
Culture Conditions ◽  
Polysaccharide Production

scholarly journals Enhancement of 2,3-Butanediol Production byKlebsiella oxytocaPTCC 1402

2011 ◽  
Vol 2011 ◽  
pp. 1-7 ◽  
Author(s):  
Maesomeh Anvari ◽  
Mohammad Reza Safari Motlagh
Keyword(s):  
Acetic Acid ◽  
Taguchi Method ◽  
Submerged Culture ◽  
Klebsiella Oxytoca ◽  
Culture Conditions ◽  
Inoculum Size ◽  
Medium Composition ◽  
Operating Parameters ◽  
Optimal Operating ◽  
Mixing Intensity

Optimal operating parameters of 2,3-Butanediol production usingKlebsiella oxytocaunder submerged culture conditions are determined by using Taguchi method. The effect of different factors including medium composition, pH, temperature, mixing intensity, and inoculum size on 2,3-butanediol production was analyzed using the Taguchi method in three levels. Based on these analyses the optimum concentrations of glucose, acetic acid, and succinic acid were found to be 6, 0.5, and 1.0 (% w/v), respectively. Furthermore, optimum values for temperature, inoculum size, pH, and the shaking speed were determined as 37°C, 8 (g/L), 6.1, and 150 rpm, respectively. The optimal combinations of factors obtained from the proposed DOE methodology was further validated by conducting fermentation experiments and the obtained results revealed an enhanced 2,3-Butanediol yield of 44%.

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