The amino acid sequence of plastocyanin from spinach. (Spinacia oleracea L.)
Keyword(s):
The amino acid sequence of spinach (Spinacia oleracea L.) plastocyanin was determined. It consists of a single polypeptide chain of 99 residues and has a sequence molecular weight of 10415. The sequence was determined by using a Beckman 890C automatic sequencer and by the dansyl--phenyl isothiocyanate analysis of peptides obtained by the enzymic digestion of purified CNBr fragments. Overlap through the two methionine residues was not shown. Sedimentation equilibrium in the ultracentrifuge gave a molecular weight for spinach plastocyanin of about 9000, in contrast with the value of 21000 reported previously by Katoh et al. (1962).
1977 ◽
Vol 79
(4)
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pp. 1212-1218
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1972 ◽
Vol 50
(12)
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pp. 1311-1325
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1984 ◽
Vol 62
(9)
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pp. 908-913
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