Multiple deoxyribonucleic acid polymerases from quiescent wheat embryos. Purification and characterization of three enzymes from the soluble cytoplasm and one from purified mitochondria
Keyword(s):
Three DNA polymerases (A, B and C) have been purified from the soluble cytoplasm of ungerminated embryos. Mainly on the basis of chromatographic, template-specificity and salt-inhibition evidence, we have characterized the three enzymes. Other physico-chemical and enzymic properties are described. From purified mitochondria we have purified a DNA polymerase that behaves like DNA polymerase B on chromatographic and template-specificity criteria. Only highly purified enzyme B from the soluble cytoplasm showed an exonuclease activity able to degrade 3′- or 5′-labelled polydeoxyribonucleotides, as well as a ‘proof-reading’ capacity.
1970 ◽
Vol 13
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pp. 20-27
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1974 ◽
Vol 50
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pp. 41-47
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1985 ◽
Vol 260
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pp. 10406-10411
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1994 ◽
Vol 1219
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pp. 267-276
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1979 ◽
Vol 98
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pp. 231-244
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