scholarly journals A synthetic oestrogen antagonist, tamoxifen, inhibits oestrogen-induced transcriptional, but not post-transcriptional, regulation of gene expression

1996 ◽  
Vol 313 (1) ◽  
pp. 269-274 ◽  
Author(s):  
Yukitomo ARAO ◽  
Etsuko YAMAMOTO ◽  
Naoto MIYATAKE ◽  
Yuichi NINOMIYA ◽  
Taisuke UMEHARA ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Oestrogen Receptor ◽  
Target Genes ◽  
Limit Of Detection ◽  
Regulation Of Gene Expression ◽  
Rapid Loss ◽  
Mrna Stabilization ◽  
Transcription Inhibitor ◽  
Post Transcriptional Regulation

Oestrogen (E2) regulates the expression of its target genes at transcriptional and post-transcriptional levels. To clarify the mechanism of E2-induced post-transcriptional regulation, with attention to the involvement of the oestrogen receptor (ER), we studied the effect of tamoxifen (TAM), a synthetic E2 antagonist that inhibits ER-mediated transcription, on E2-induced transcriptional and post-transcriptional regulation of the chicken ovalbumin (OVA) gene in chick oviducts. Run-on analysis with oviduct nuclei isolated from E2-treated chicks showed that TAM treatment completely blocked E2-induced transcription of the OVA gene within 24 h without affecting ER gene expression. Likewise, the rate of transcription fell to below the limit of detection after E2 withdrawal from the chicks. Reflecting the transcription rate, OVA mRNA accumulated linearly in E2-treated chicks, and E2 withdrawal caused a rapid loss of OVA mRNA. However, in the chicks treated with TAM and E2, OVA mRNA was degraded slowly over 48 h with a half-life of 24 h, suggesting that TAM does not inhibit E2-induced mRNA stabilization. Moreover, E2-induced mRNA stabilization was observed even when transcription of the OVA gene was blocked by a transcription inhibitor. Western-blot analysis showed that the remaining OVA mRNA was translatable. Thus the present study indicates that E2 regulates expression of the OVA gene via distinct pathways at transcriptional and post-transcriptional levels.

Post-transcriptional regulation of gene expression

Methods ◽  
2017 ◽  
Vol 126 ◽  
pp. 1-2 ◽  
Author(s):  
Howard D. Lipshitz ◽  
Julie M. Claycomb ◽  
Craig A. Smibert
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Regulation Of Gene Expression ◽  
Post Transcriptional Regulation

scholarly journals Post-transcriptional regulation of gene expression by degradation of messenger RNAs

2003 ◽  
Vol 195 (3) ◽  
pp. 356-372 ◽  
Author(s):  
Annamaria Bevilacqua ◽  
Maria Cristina Ceriani ◽  
Sergio Capaccioli ◽  
Angelo Nicolin
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Regulation Of Gene Expression ◽  
Messenger Rnas ◽  
Post Transcriptional Regulation

scholarly journals Regulatory signals in messenger RNA: determinants of nutrient–gene interaction and metabolic compartmentation

1998 ◽  
Vol 80 (4) ◽  
pp. 307-321
Author(s):  
John E. Hesketh ◽  
M. Helena Vasconcelos ◽  
Giovanna Bermano
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Mrna Stability ◽  
Transcriptional Control ◽  
Regulation Of Gene Expression ◽  
Untranslated Regions ◽  
Nutritional Requirements ◽  
Control Of Gene Expression ◽  
Metabolic Compartmentation ◽  
Post Transcriptional Regulation

Nutrition has marked influences on gene expression and an understanding of the interaction between nutrients and gene expression is important in order to provide a basis for determining the nutritional requirements on an individual basis. The effects of nutrition can be exerted at many stages between transcription of the genetic sequence and production of a functional protein. This review focuses on the role of post-transcriptional control, particularly mRNA stability, translation and localization, in the interactions of nutrients with gene expression. The effects of both macronutrients and micronutrients on regulation of gene expression by post-transcriptional mechanisms are presented and the post-transcriptional regulation of specific genes of nutritional relevance (glucose transporters, transferrin, selenoenzymes, metallothionein, lipoproteins) is described in detail. The function of the regulatory signals in the untranslated regions of the mRNA is highlighted in relation to control of mRNA stability, translation and localization and the importance of these mRNA regions to regulation by nutrients is illustrated by reference to specific examples. The localization of mRNA by signals in the untranslated regions and its function in the spatial organization of protein synthesis is described; the potential of such mechanisms to play a key part in nutrient channelling and metabolic compartmentation is discussed. It is concluded that nutrients can influence gene expression through control of the regulatory signals in these untranslated regions and that the post-transcriptional regulation of gene expression by these mechanisms may influence nutritional requirements. It is emphasized that in studies of nutritional control of gene expression it is important not to focus only on regulation through gene promoters but also to consider the possibility of post-transcriptional control.

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