RADIOACTIVITY OF CHOLESTEROL AND OF NON LIPID FRACTIONS AFTER INCORPORATION OF LABELED UNSATURATED FATTY ACIDS IN RAT LIVER AND HUMAN SKIN FIBROBLASTS

1981 ◽  
Vol 9 (2) ◽  
pp. 184P-184P
Author(s):  
J. Raulin ◽  
M. Bouchène ◽  
D. Lapous ◽  
C. Wolfrom ◽  
M. Gautier
Keyword(s):  
Fatty Acids ◽  
Human Skin ◽  
Rat Liver ◽  
Unsaturated Fatty Acids ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Lipid Fractions

scholarly journals Formic acid is a product of the α-oxidation of fatty acids by human skin fibroblasts: deficiency of formic acid production in peroxisome-deficient fibroblasts

1993 ◽  
Vol 292 (2) ◽  
pp. 457-461 ◽  
Author(s):  
A Poulos ◽  
P Sharp ◽  
H Singh ◽  
D W Johnson ◽  
W F Carey ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Formic Acid ◽  
Human Skin ◽  
Acid Production ◽  
Phytanic Acid ◽  
Culture Medium ◽  
Water Soluble ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Beta Oxidation

Human skin fibroblasts in culture can oxidize beta-methyl fatty acids, such as phytanic acid and 3-methylhexadecanoic acid, to CO2 and water-soluble products. The latter are released largely into the culture medium. The major water-soluble product formed from [1-14C]phytanic and [1-14C]3-methylhexadecanoic acids is [14C]formic acid. As phytanic acid and 3-methylhexadecanoic acids contain beta-methyl groups and theoretically cannot be degraded by beta-oxidation, we postulate that formic acid is formed from fatty acids by alpha-oxidation. The marked reduction in formic acid production from beta-methyl fatty acids in peroxisome-deficient skin fibroblasts suggests that peroxisomes are involved in the generation of C1 units.

scholarly journals Conversion of epoxyeicosatrienoic acids (EETs) to chain-shortened epoxy fatty acids by human skin fibroblasts

2000 ◽  
Vol 41 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Xiang Fang ◽  
Terry L. Kaduce ◽  
Mike VanRollins ◽  
Neal L. Weintraub ◽  
Arthur A. Spector
Keyword(s):  
Fatty Acids ◽  
Human Skin ◽  
Skin Fibroblasts ◽  
Epoxyeicosatrienoic Acids ◽  
Human Skin Fibroblasts ◽  
Epoxy Fatty Acids

The effects oftrans fatty acids on fatty acyl Δ5 desaturation by human skin fibroblasts

Lipids ◽  
1984 ◽  
Vol 19 (11) ◽  
pp. 869-874 ◽  
Author(s):  
Miriam D. Rosenthal ◽  
Mark A. Doloresco
Keyword(s):  
Fatty Acids ◽  
Human Skin ◽  
Fatty Acyl ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts

Cholesterol-mediated regulation of HMG-CoA reductase in microsomes from human skin fibroblasts and rat liver

1990 ◽  
Vol 68 (3) ◽  
pp. 674-679 ◽  
Author(s):  
R. George ◽  
P. J. Davis ◽  
L. Luong ◽  
M. J. Poznansky
Keyword(s):  
Tissue Culture ◽  
Enzyme Activity ◽  
Human Skin ◽  
Rat Liver ◽  
Reductase Activity ◽  
Cholesterol Content ◽  
Skin Fibroblasts ◽  
Human Skin Fibroblasts ◽  
Hmg Coa Reductase ◽  
Coa Reductase

3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase activity was determined in microsomes from human skin fibroblasts and rat liver that had been variously manipulated in vivo or in tissue culture to up- and down-regulate the enzyme. The cholesterol content of these microsomal preparations was then altered by depletion to or enrichment from either cholesterol-free or cholesterol-rich lipid vesicles. Microsomes from human skin fibroblasts responded to cholesterol depletion by increasing HMG-CoA reductase activity and by decreasing it in response to cholesterol enrichment. This was independent of the initial enzyme activity or the tissue culture conditions. Alterations in cholesterol content of rat liver microsomes in vitro failed to demonstrate any significant changes in HMG-CoA reductase activity whether the microsomes started with low enzyme activity (cholesterol-fed rats) or with high enzyme activity (cholestyramine-treated rats). The results are discussed in relation to previously published data and in respect to differences in the control of the human skin fibroblast and rat liver enzymes.Key words: cholesterol, HMG-CoA reductase, microsomes, fibroblasts, rat liver.

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