Solid-phase enzyme-linked immunosorbent assay for the large scale-screening of hybridomas: non-specific effects and their solutions

1985 ◽  
Vol 13 (2) ◽  
pp. 473-474 ◽  
Author(s):  
KENNETH CARROLL ◽  
RICHARD O'KENNEDY
Keyword(s):  
Immunosorbent Assay ◽  
Large Scale ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Effects ◽  
Large Scale Screening

Mechanization of the enzyme-linked immunosorbent assay (ELISA) for large scale screening of sera

1977 ◽  
Vol 16 (4) ◽  
pp. 351-359 ◽  
Author(s):  
E.J. Ruitenberg ◽  
J.A. van Amstel ◽  
B.J.M. Brosi ◽  
P.A. Steerenberg
Keyword(s):  
Immunosorbent Assay ◽  
Large Scale ◽  
Enzyme Linked Immunosorbent Assay ◽  
Large Scale Screening

National survey of viruses in pastures of subterranean clover. III. Equipment for large scale screening of viruses by ELISA

1993 ◽  
Vol 44 (8) ◽  
pp. 1883 ◽  
Author(s):  
LB Hulse ◽  
K Helms ◽  
PM Waterhouse
Keyword(s):  
Immunosorbent Assay ◽  
Large Scale ◽  
New South ◽  
South Australia ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Nationwide Survey ◽  
Enzyme Linked Immunosorbent Assay ◽  
South Wales ◽  
Large Scale Screening

Equipment for large scale enzyme-linked immunosorbent assay (ELISA) was developed for a 3 year nationwide survey of the incidence of four viruses in pastures of Trifolium subterraneum (subterranean clover) in New South Wales, Victoria, South Australia, Western Australia and Tasmania. Instruments were designed to (a) automate quantitatively the addition of buffer to plant samples passing through a sap extractor, (b) standardize the washing of plates in the various stages of the ELISA protocol, and (c) replace hand pipetting with a pneumatic foot-operated pipette.

A New Technique in Quantitative Immunohematology: Solid-Phase Kinetic Enzyme-Linked Immunosorbent Assay

Vox Sanguinis ◽  
1983 ◽  
Vol 45 (6) ◽  
pp. 440-448 ◽  
Author(s):  
S. Spitalnik ◽  
J. Cowles ◽  
M.T. Cox ◽  
D. Baker ◽  
J. Holt ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Solid Phase ◽  
New Technique ◽  
Enzyme Linked Immunosorbent Assay ◽  
A New Technique

Enzyme-linked immunosorbent assay for detection of antibodies to extractable nuclear antigens in systemic lupus erythematosus, with nylon as solid phase.

1983 ◽  
Vol 29 (5) ◽  
pp. 823-827 ◽  
Author(s):  
M Ishaq ◽  
R Ali
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Immunosorbent Assay ◽  
Lupus Erythematosus ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Prolonged Storage ◽  
Antibody Activity ◽  
Systemic Lupus ◽  
Nuclear Antigens ◽  
As Solid Phase

Abstract In this enzyme-linked immunosorbent assay (ELISA) for detection of antibodies against extractable nuclear antigens (ENA) in sera of patients with systemic lupus erythematosus (SLE), nylon is used as solid phase for antigen binding instead of the commonly used polystyrene surface. Optimal conditions for activation of the nylon beads, antigen coating, and other relevant factors have been investigated. We compared the incidence of anti-ENA antibodies in SLE, using chromogenic and fluorogenic enzyme substrates. Of SLE patients, 54% were positive for anti-ENA antibodies when chromogenic substrate was used as compared with 68% for fluorogenic substrate. Antibody activity against Sm and RNP antigens was distinguished on the basis of ribonuclease sensitivity of the RNP antigen. The method described offers advantages such as decreased background activity, increased surface area, facility for prolonged storage of antigen-coated solid phase, and miniaturization of the assay.

A solid-phase enzyme-linked immunosorbent assay for the quantitation of human plasma alpha 1-acid glycoprotein.

1979 ◽  
Vol 25 (4) ◽  
pp. 546-549 ◽  
Author(s):  
H P Wang ◽  
C Y Chu
Keyword(s):  
Alkaline Phosphatase ◽  
Human Plasma ◽  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Solid Phase ◽  
Enzyme Linked Immunosorbent Assay ◽  
Normal Range ◽  
Oral Contraceptive Pills ◽  
Acid Glycoprotein ◽  
Contraceptive Pills

Abstract We describe a solid-phase enzyme-linked immunosorbent assay for alpha 1-acid glycoprotein in human plasma. Plasma samples are incubated with alkaline phosphatase-linked, purified alpha 1-acid glycoprotein in alpha 1-acid glycoprotein-specific antibody-coated polystyrene tubes. The alkaline phosphatase that becomes attached to the tube via an immunological reaction between the alpha 1-acid glycoprotein and the specific antibody is measured spectrophotometrically. This assay is accurate reproducible, simple, and economical. As little as 4 microgram of alpha 1-acid glycoprotein per liter can be detected. The normal range for alpha 1-acid glycoprotein in the plasma of healthy adults, as measured by this method, is 0.48-1.27 g/L; the range is significantly different, 0.29-0.73 g/L, for women who are taking oral contraceptive pills.

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