Solid-phase enzyme-linked immunosorbent assay for hepatitis E virus IgG and IgM antibodies utilizing recombinant antigens and synthetic peptides

1992 ◽  
Vol 38 (1) ◽  
pp. 175-186 ◽  
Author(s):  
George J. Dawson ◽  
Kurt H. Chau ◽  
Carlos M. Cabal ◽  
Patrice O. Yarbough ◽  
Gregory R. Reyes ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Synthetic Peptides ◽  
Hepatitis E Virus ◽  
Solid Phase ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Recombinant Antigens ◽  
Igm Antibodies

scholarly journals Good Performance of Immunoglobulin M Assays in Diagnosing Genotype 3 Hepatitis E Virus Infections

2009 ◽  
Vol 16 (5) ◽  
pp. 772-774 ◽  
Author(s):  
Florence Legrand-Abravanel ◽  
Isabelle Thevenet ◽  
Jean-Michel Mansuy ◽  
Karine Saune ◽  
Françoise Vischi ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis E Virus ◽  
Rapid Test ◽  
Hepatitis E ◽  
Immunoglobulin M ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Genotype 3

ABSTRACT We have evaluated three anti-hepatitis E virus (anti-HEV) immunoglobulin M (IgM) assays, the EIAgen HEV IgM assay (Adaltis), the HEV IgM enzyme-linked immunosorbent assay 3.0, and the Assure HEV IgM rapid test (MP Diagnostics), for the routine detection of acute genotype 3 HEV. Their sensitivities were fairly good (90%, 88%, and 82%), and their specificities were excellent (100%, 99.5%, and 100%).

Recombinant Antigens and Synthetic Peptides for Serodiagnosis of Hepatitis E Virus Infection

1994 ◽  
pp. 371-374 ◽  
Author(s):  
G. Dawson ◽  
D. Paul ◽  
R. Gutierrez ◽  
T. Pilot-Matias ◽  
K. Chau ◽  
...  
Keyword(s):  
Virus Infection ◽  
Synthetic Peptides ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Recombinant Antigens

scholarly journals Comparison of a New Immunochromatographic Test to Enzyme-Linked Immunosorbent Assay for Rapid Detection of Immunoglobulin M Antibodies to Hepatitis E Virus in Human Sera

2005 ◽  
Vol 12 (5) ◽  
pp. 593-598 ◽  
Author(s):  
Hsiao Ying Chen ◽  
Yang Lu ◽  
Teresa Howard ◽  
David Anderson ◽  
Priscilla Yiquan Fong ◽  
...  
Keyword(s):  
Acute Hepatitis ◽  
Immunosorbent Assay ◽  
Predictive Value ◽  
Rapid Detection ◽  
Hepatitis E Virus ◽  
Rapid Test ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunochromatographic Test ◽  
Acute Hepatitis E

ABSTRACT An immunochromatographic test for rapid detection of IgM antibodies in patients with acute hepatitis E infection was developed utilizing the well-characterized recombinant protein EP2.1 and monoclonal antibody 4B2. The new rapid test based on a novel reverse-flow technology was able to generate a positive result within 2 to 3 min. Our study showed that this test was able to detect anti-HEV IgM antibodies in 96.7% of the patient samples tested (n = 151) while maintaining an excellent specificity of 98.6% with samples from various patient or healthy control groups (total n = 208). Furthermore, this rapid test gave a good specificity of 90.9% when tested with rheumatoid factor (RF)-positive sera (RF value of ≤850 IU/ml; n = 11) although a higher concentration of RF in samples might cause cross-reactivity. The new test has a good agreement of 97.2% with a kappa value of 0.943 when compared with a reference enzyme-linked immunosorbent assay. The positive predictive value and the negative predictive value for the rapid test thus reached 98.0 and 97.6%, respectively. This is the first rapid, point-of-care test for hepatitis E and will be especially useful for the diagnosis of acute hepatitis E virus infection in field and emergency settings and in resource-poor countries.

scholarly journals Line immunoassay for detection of IgG antibodies to hepatitis E virus (Hepeviridae, Orthohepevirus, Orthohepevirus A)

2020 ◽  
Vol 65 (3) ◽  
pp. 132-142
Author(s):  
G. I. Alatortseva ◽  
V. V. Dotsenko ◽  
L. N. Nesterenko ◽  
L. N. Luhverchik ◽  
V. Yu. Kabargina ◽  
...  
Keyword(s):  
Human Serum ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Test System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Analytical Sensitivity ◽  
Igg Antibodies ◽  
Recombinant Antigens ◽  
Test Kit ◽  
Wide Range

Introduction. The diagnostic efficacy of methods for hepatitis E serodiagnostic varies over a wide range; therefore, the combined use of tests of various formats is recommended. The aim of the research was to develop a test system for the detection of IgG antibodies to hepatitis E virus (HEV) in human serum by linear immunoassay (LIA). Material and methods. Serum samples from patients with hepatitis and healthy individuals were tested using commercial enzyme-linked immunosorbent assay systems for the presence of IgG antibodies to viral agents causing hepatitis and other infections associated with liver pathology. Recombinant antigens ORF2 and ORF3 of HEV genotypes 1 and 3 were used. The “RecomLine HEV IgG/IgM” reagent kit (Mikrogen GmbH, Germany) was used as a comparison test system. Results. The first Russian diagnostic kit “Blot-HEV”, designed to detect IgG antibodies to individual HEV proteins in human serum using LIA, was developed. The antigenic base is represented by strips of a nitrocellulose membrane with immobilized recombinant antigens ORF2 (aa 406–660) and ORF3 (aa 1–113) of HEV genotypes 1 and 3, and control antigens in the form of discrete lines. The conjugate was mouse monoclonal antibodies to human class G immunoglobulins labeled with horseradish peroxidase. The chromogen solution contained the 3,3’,5,5’-tetramethylbenzidine. A visual and digital recording of results was provided. The analytical sensitivity of the test kit was 0.625 IU/ml for ORF2 antigens and 2.5 IU/ml for ORF3 antigens. The absence of the influence of endogenous interfering substances on the results of the analysis and the absence of cross-reactions with antibodies to hepatitis pathogens of the other etiologies had been shown. The sensitivity of the test system compared to the “RecomLine HEV IgG/IgM” kit was 92%, specificity 97%. Shelf life in condition of storage was determined to be 12 months. Conclusions. The developed test can be used to confirm the results of ELISA in laboratory diagnosis of hepatitis E.

Empty virus-like particle-based enzyme-linked immunosorbent assay for antibodies to hepatitis E virus

2000 ◽  
Vol 62 (3) ◽  
pp. 327-333 ◽  
Author(s):  
Tian-Cheng Li ◽  
Jing Zhang ◽  
Haruhide Shinzawa ◽  
Masamichi Ishibashi ◽  
Michio Sata ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Hepatitis E Virus ◽  
Hepatitis E ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Like Particle

Enzyme-linked immunosorbent assay for serum amyloid A apolipoprotein with use of specific antibodies against synthetic peptides.

1988 ◽  
Vol 34 (9) ◽  
pp. 1767-1771 ◽  
Author(s):  
R Saïle ◽  
C Delpierre ◽  
P Puchois ◽  
G Hocke ◽  
C Cachera ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Synthetic Peptides ◽  
Solid Phase ◽  
Polyclonal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Antibodies ◽  
Amyloid A ◽  
Microtiter Plates ◽  
Sensitivity Specificity ◽  
As Solid Phase

Abstract We describe a noncompetitive enzyme-linked immunosorbent assay for amyloid A apolipoprotein in human serum (apo SAA) in which specific antibodies against synthetic peptides are used. Microtiter plates were used as solid phase and coated with affinity-purified antibodies raised against SAA1-(95-104) peptide. After incubation with delipidated plasmas, the bound apo SAA was revealed by labeled antibodies raised against SAA1-(58-69) peptide. The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, and non-use of radioisotopes. Results correlate well with those by a nephelometric method in which polyclonal antibodies are used.

Sign in / Sign up

Export Citation Format

Share Document