The molecular basis of the high linoleic acid content in Petunia seed oil: analysis of a seed-specific linoleic acid mutant

2000 ◽  
Vol 28 (6) ◽  
pp. 631-632 ◽  
Author(s):  
I. Verwoert ◽  
Y. Meller-Harel ◽  
K. van der Linden ◽  
B. Verbree ◽  
R. Koes ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Acid Content ◽  
Seed Oil ◽  
Lipid Fraction ◽  
Lipid Synthesis ◽  
Linoleic Acid Content ◽  
Specific Expression ◽  
High Linoleic Acid ◽  
High Linoleic Acid Content ◽  
Δ12 Desaturase

From a random transposon mutagenesis experiment, using Petunia line W138, a seed-specific linoleic acid mutant was isolated. The tagged gene was cloned and identified as a microsomal Δ12 desaturase. Expression of the gene, however, was constitutive and not, as might have been expected, seed-specific. Moreover, self-fertilized homozygous mutants still contain 40% 18:2 in the seed lipid fraction. This suggests that at least two (seedspecific) Δ12 desaturase genes are responsible for the high linoleic acid content in Petunia seed oil. Five members of the microsomal Δ12 desaturase gene family have been identified and isolated. Data are presented on the molecular characterization and tissue-specific expression of these genes, which suggest that, in Petunia the flux through the prokaryotic and eukaryotic pathways of lipid synthesis might be different from the situation found in Arabidopsis.

MEAT WITH HIGH LINOLEIC ACID CONTENT: OXIDATIVE CHANGES DURING FROZEN STORAGE

2008 ◽  
Vol 41 (4) ◽  
pp. 757-761 ◽  
Author(s):  
H. A. BREMNER ◽  
A. L. FORD ◽  
J. J. MACFARLANE ◽  
D. RATCLIFF ◽  
N. T. RUSSELL
Keyword(s):  
Linoleic Acid ◽  
Acid Content ◽  
Frozen Storage ◽  
Linoleic Acid Content ◽  
High Linoleic Acid ◽  
High Linoleic Acid Content

Selection of High Linoleic Acid Content in Summer Turnip Rape (Brassica campestrisL.)

1982 ◽  
Vol 32 (4) ◽  
pp. 397-404 ◽  
Author(s):  
I. Laakso ◽  
R. Hiltunen ◽  
S. Hovinen ◽  
M. Von Schantz ◽  
A. Huhtikangas
Keyword(s):  
Linoleic Acid ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
High Linoleic Acid ◽  
Turnip Rape ◽  
High Linoleic Acid Content ◽  
Selection Of

Selection for high linoleic acid content in sunflower (Helianthus annuus L.)

1989 ◽  
Vol 29 (2) ◽  
pp. 233 ◽  
Author(s):  
BW Simpson ◽  
CM McLeod ◽  
DL George
Keyword(s):  
Linoleic Acid ◽  
Acid Content ◽  
Linoleic Acid Content ◽  
High Linoleic Acid ◽  
Breeding Lines ◽  
Wide Range ◽  
Total Fatty Acids ◽  
High Linoleic Acid Content ◽  
Selection For ◽  
Non Destructive

Inbred sunflower lines with high and stable levels of linoleic acid over a wide range of maturation temperatures have been produced. Normally linoleic acid (the polyunsaturated component of sunflower oil) is inversely proportional to temperature during seed maturation. During Queensland's hot summers, linoleic acid content may drop to <50%. Processors require a linoleic acid content of at least 62% of the total fatty acids in oil used for the production of polyunsaturated products, e.g. margarine. Four generations of breeding material (F2-F6 of Hysun 33xHysun 32) were grown and matured under hot summer conditions (approximately 30�C max., 18�C min.). The fatty acid composition of individual seeds was determined by non-destructive analysis, and sunflower breeding lines with up to 25% higher (actual) linoleic acid than the standard variety Hysun 32 (which averaged 48% linoleic acid when matured under hot summer conditions) have been produced.

Investigation of RCCI Characteristics of Combustion and Emissions With PFI of N-Butanol and Direct Injection of High Linoleic Content Biodiesel

2016 ◽  
Author(s):  
Valentin Soloiu ◽  
Tyler Naes ◽  
Martin Muiños ◽  
Alejandro Rivero-Castillo ◽  
Spencer Harp
Keyword(s):  
Linoleic Acid ◽  
Heat Release ◽  
Acid Content ◽  
Ignition Delay ◽  
Fuel Injection ◽  
Direct Injection ◽  
Linoleic Acid Content ◽  
High Linoleic Acid ◽  
High Linoleic Acid Content ◽  
Soot Emissions

This study investigated the RCCI (Reactivity Controlled Compression Ignition) of PFI (Port Fuel Injection) of n-butanol with direct injection (DI) of a high linoleic acid content biodiesel, cottonseed (CS100). The experimental omnivorous-fuel engine was operated at 1400 rpm and 6 bar indicated mean effective pressure (IMEP) with 20% cooled EGR. The mass ratio of n-butanol injected comprised of 50% of the total fuel mass. The dual fueling strategy of RCCI changed the conventional diesel combustion (CDC) apparent heat release profile. With the new fueling strategy the heat release was split into two regions of high temperature heat release when using CS100. The first occurred before top dead center (BTDC) from the high reactivity fuel and the second peak occurred due to the combustion of the low reactivity fuel (n-butanol) after top dead center (ATDC). ULSD did not produce this same split in heat release due to the longer ignition delay. The ignition delay for CS100 was shortened by 0.15 ms when compared to ULSD because of the high palmitic acid content in the biodiesel. The RCCI process itself extended the ignition delay about 0.17 ms (about 1.4 CAD) suggesting the possibility of controlling combustion phasing for both RCCI fueling strategies of ULSD and CS100. The CA50 occurred similarly at 10° ATDC for ULSD and CS100 however, RCCI shifted the CA50’s (7° and 8° ATDC for CS100 and ULSD respectively). Soot emissions exhibited a decrease with the PFI of n-butanol because of the highly oxygenated nature of the fuel by 80%. In summary, RCCI stratification using n-Butanol as the low reactivity fuel significantly reduced soot emissions when using either a high linoleic acid content biofuel or ULSD while also suggesting control over combustion phasing.

Evaluation of wild sunflower (Helianthus) species for high content and stability of linoleic acid in the seed oil

1991 ◽  
Vol 116 (3) ◽  
pp. 359-367 ◽  
Author(s):  
A. De Haro ◽  
J. Fernandez-Martinez
Keyword(s):  
Linoleic Acid ◽  
Acid Content ◽  
Seed Oil ◽  
Oil Quality ◽  
Linoleic Acid Content ◽  
Seed Formation ◽  
Warm Environment ◽  
Mean Temperature ◽  
Breeding Programmes ◽  
Helianthus Species

SUMMARYA collection of 168 accessions belonging to 62 species and subspecies was evaluated in Cordoba, Spain, in 1985 for fatty acid composition of the seed oil. Linoleic acid content of seed produced in Cordoba (mean temperature during seed formation 27·9 °C) was compared with that of seed obtained under much cooler environmental conditions in Montpellier, France, (mean temperature 19·5 °C). Linoleic acid content ranged from 27·3 to 83·7% in the warm environment and from 45·9 to 88·7% in the cool environment with average values of 64·3% and 75·0%, respectively. Twenty-three species with linoleic acid values of > 70% in both environments were evaluated again, in 1989 in Cordoba (mean temperature 31 °C). Twelve wild species showed consistently high and more stable linoleic acid contents than the cultivated control and could be readily used in breeding programmes to improve oil quality.

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