scholarly journals Palmitoylation-dependent regulation of glutamate receptors and their PDZ domain-containing partners

2013 ◽  
Vol 41 (1) ◽  
pp. 72-78 ◽  
Author(s):  
Gareth M. Thomas ◽  
Richard L. Huganir
Keyword(s):  
Synaptic Transmission ◽  
Glutamate Receptors ◽  
Glutamate Receptor ◽  
Pdz Domain ◽  
Receptor Trafficking ◽  
Ionotropic Glutamate Receptor ◽  
Novel Treatments ◽  
Neuronal Regulation ◽  
Glutamate Receptor Trafficking ◽  
And Function

In recent years, it has become clear that both AMPA (α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid)- and NMDA (N-methyl-D-aspartate)-type glutamate receptors, and many of their interacting partners, are palmitoylated proteins. Interfering with palmitoylation dramatically affects receptor trafficking and distribution and, in turn, can profoundly alter synaptic transmission. Increased knowledge of synaptic palmitoylation not only will aid our understanding of physiological neuronal regulation, but also may provide insights into, and even novel treatments for, neuropathological conditions. In the present paper, we review recent advances regarding the regulation of ionotropic glutamate receptor trafficking and function by palmitoylation.

scholarly journals Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Kento Ojima ◽  
Kazuki Shiraiwa ◽  
Kyohei Soga ◽  
Tomohiro Doura ◽  
Mikiko Takato ◽  
...  
Keyword(s):  
Glutamate Receptors ◽  
Glutamate Receptor ◽  
Receptor Trafficking ◽  
Protein Labeling ◽  
Selective Labeling ◽  
Receptor Localization ◽  
Glutamate Receptor Trafficking ◽  
Versatile Tool ◽  
The Central Nervous System ◽  
Long Lifetime

AbstractThe regulation of glutamate receptor localization is critical for development and synaptic plasticity in the central nervous system. Conventional biochemical and molecular biological approaches have been widely used to analyze glutamate receptor trafficking, especially for α-amino-3-hydroxy-5-methyl-4-isoxazole-propionate-type glutamate receptors (AMPARs). However, conflicting findings have been reported because of a lack of useful tools for analyzing endogenous AMPARs. Here, we develop a method for the rapid and selective labeling of AMPARs with chemical probes, by combining affinity-based protein labeling and bioorthogonal click chemistry under physiological temperature in culture medium. This method allows us to quantify AMPAR distribution and trafficking, which reveals some unique features of AMPARs, such as a long lifetime and a rapid recycling in neurons. This method is also successfully expanded to selectively label N-methyl-D-aspartate-type glutamate receptors. Thus, bioorthogonal two-step labeling may be a versatile tool for investigating the physiological and pathophysiological roles of glutamate receptors in neurons.

scholarly journals Crystal structures of autoinhibitory PDZ domain of Tamalin: implications for metabotropic glutamate receptor trafficking regulation

2007 ◽  
Vol 26 (8) ◽  
pp. 2192-2205 ◽  
Author(s):  
Takuma Sugi ◽  
Takuji Oyama ◽  
Takanori Muto ◽  
Shigetada Nakanishi ◽  
Kosuke Morikawa ◽  
...  
Keyword(s):  
Crystal Structures ◽  
Glutamate Receptor ◽  
Metabotropic Glutamate Receptor ◽  
Pdz Domain ◽  
Receptor Trafficking ◽  
Metabotropic Glutamate ◽  
Glutamate Receptor Trafficking

scholarly journals Ligand-directed two-step labeling to quantify neuronal glutamate receptor trafficking

2020 ◽  
Author(s):  
Kento Ojima ◽  
Kazuki Shiraiwa ◽  
Tomohiro Doura ◽  
Mikiko Takato ◽  
Kazuhiro Komatsu ◽  
...  
Keyword(s):  
Glutamate Receptors ◽  
Glutamate Receptor ◽  
Receptor Trafficking ◽  
Protein Labeling ◽  
Selective Labeling ◽  
Receptor Localization ◽  
Glutamate Receptor Trafficking ◽  
Versatile Tool ◽  
The Central Nervous System ◽  
Long Lifetime

ABSTRACTThe regulation of glutamate receptor localization is critical for development and synaptic plasticity in the central nervous system. Conventional biochemical and molecular biological approaches have been widely used to analyze glutamate receptor trafficking, especially for AMPA-type glutamate receptors (AMPARs). However, conflicting findings have been reported because of a lack of useful tools for analyzing endogenous AMPARs. Here, we develop a new method for the rapid and selective labeling of chemical probes to AMPARs by combining affinity-based protein labeling and bioorthogonal click chemistry under physiological conditions. This method allowed us to quantify AMPAR distribution and trafficking, which revealed some unique features of AMPARs, such as a long lifetime and a rapid recycling in neurons. This method was also successfully expanded to selectively label NMDA-type glutamate receptors. Thus, bioorthogonal two-step labeling may be a versatile tool for investigating the physiological and pathophysiological roles of glutamate receptors in neurons.

scholarly journals More than meets the IR: the expanding roles of variant Ionotropic Glutamate Receptors in sensing odor, taste, temperature and moisture

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1753 ◽  
Author(s):  
Lena van Giesen ◽  
Paul A. Garrity
Keyword(s):  
Glutamate Receptors ◽  
Glutamate Receptor ◽  
Olfactory Receptors ◽  
Sensory Transduction ◽  
Ionotropic Glutamate Receptors ◽  
Ionotropic Glutamate Receptor ◽  
Ecological Significance ◽  
Ionotropic Receptors ◽  
Sensory Modalities ◽  
Receptor Family

The ionotropic receptors (IRs) are a branch of the ionotropic glutamate receptor family and serve as important mediators of sensory transduction in invertebrates. Recent work shows that, though initially studied as olfactory receptors, the IRs also mediate the detection of taste, temperature, and humidity. Here, we summarize recent insights into IR evolution and its potential ecological significance as well as recent advances in our understanding of how IRs contribute to diverse sensory modalities.

scholarly journals Role for A Kinase-anchoring Proteins (AKAPS) in Glutamate Receptor Trafficking and Long Term Synaptic Depression

2005 ◽  
Vol 280 (17) ◽  
pp. 16962-16968 ◽  
Author(s):  
Eric M. Snyder ◽  
Marcie Colledge ◽  
Robert A. Crozier ◽  
Wendy S. Chen ◽  
John D. Scott ◽  
...  
Keyword(s):  
Glutamate Receptor ◽  
Synaptic Depression ◽  
Receptor Trafficking ◽  
Anchoring Proteins ◽  
Glutamate Receptor Trafficking

scholarly journals Glutamate Receptor Trafficking and Protein Synthesis Mediate the Facilitation of LTP by Secreted Amyloid Precursor Protein-Alpha

2019 ◽  
Vol 39 (17) ◽  
pp. 3188-3203 ◽  
Author(s):  
Bruce G. Mockett ◽  
Diane Guévremont ◽  
Megan K. Elder ◽  
Karen D. Parfitt ◽  
Katie Peppercorn ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amyloid Precursor Protein ◽  
Glutamate Receptor ◽  
Receptor Trafficking ◽  
Precursor Protein ◽  
Glutamate Receptor Trafficking
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