Observations on the Passage of Apoproteins from Plasma Lipoproteins into Peripheral Lymph in Two Men

1975 ◽  
Vol 49 (5) ◽  
pp. 419-426 ◽  
Author(s):  
D. Reichl ◽  
A. Postiglione ◽  
N. B. Myant ◽  
J. J. Pflug ◽  
M. Press
Keyword(s):  
High Density Lipoprotein ◽  
Low Density Lipoprotein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Density Lipoprotein ◽  
High Density ◽  
Plasma Lipoproteins ◽  
Low Density ◽  
Peripheral Lymph ◽  
Apoprotein B ◽  
Plasma High Density

1. The passage of radioactive apolipoproteins into lymph draining the foot was investigated in two men, each given a single intravenous injection of low-density lipoprotein containing 131I-labelled apoprotein B and of very-low-density lipoprotein containing 125I-labelled apoprotein A and apoprotein C. 2. Protein-bound 125I and 131I appeared in the lymph of both subjects. Immunoelectrophoresis of lymph lipoproteins against anti-(high-density lipoprotein) and anti-(low-density lipoprotein) showed the presence of apo-high-density lipoprotein and apo-low-density lipoprotein with faster mobilities than plasma high-density and low-density lipoprotein respectively. Most of the protein-bound 131I in lymph was recovered in the precipitin line formed by the apoprotein B-containing lipoprotein after immunoelectrophoresis. Polyacrylamide gel electrophoresis of the lymph lipoprotein fraction showed the presence of 125I-containing bands with mobilities similar to those of the apoprotein A of high-density lipoprotein and of three of the fast-moving C apoproteins. 3. These results suggest that most, if not all, of the apoproteins of plasma lipoproteins reach the interstitial fluids and that some lipoproteins undergo modification during their passage into peripheral lymph.

scholarly journals Fluorescence studies of protein–sterol relationships in human plasma lipoproteins

1974 ◽  
Vol 137 (2) ◽  
pp. 413-415 ◽  
Author(s):  
Rory J. M. Smith ◽  
Colin Green
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Tryptophan Fluorescence ◽  
High Density ◽  
Plasma Lipoproteins ◽  
Low Density ◽  
Fluorescence Studies ◽  
Esterified Sterols

Cholesta-5,7,9(11)-trien-3β-ol and its oleate ester were incorporated into human low-density lipoprotein and reconstituted high-density lipoprotein. The unesterified sterol was more efficient than its ester in quenching tryptophan fluorescence, especially in low-density lipoprotein. The results, which indicate that in such lipoproteins unesterified sterols are more closely associated with peptide than are esterified sterols, are used to assess possible structures for the lipoproteins.

The Passage of Apoproteins from Plasma Lipoproteins into the Lipoproteins of Peripheral Lymph in Man

1977 ◽  
Vol 53 (3) ◽  
pp. 221-226
Author(s):  
D. Reichl ◽  
N. B. Myant ◽  
J. J. Pflug ◽  
D. N. Rudra
Keyword(s):  
Intravenous Injection ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Plasma Lipoproteins ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Intermediate Density Lipoprotein ◽  
Peripheral Lymph ◽  
Apoprotein B ◽  
Intermediate Density

1. The transport of apoprotein B from the lipoproteins of plasma into the lipoproteins of lymph draining the foot has been studied in four men with type III hyperlipoproteinaemia. 2. Three subjects were given autologous 125I-labelled very-low-density lipoprotein (VLDL) and 131I-labelled low-density lipoprotein (LDL) by intravenous injection; the fourth was given autologous 125I-labelled VLDL and 131I-labelled intermediate-density lipoprotein (IDL) plus LDL. 3. The 125I/131I ratios in serum and lymph apoprotein B, and the 125I and 131I specific radioactivities of apoprotein B in VLDL, IDL and LDL from serum and lymph, indicate that apoprotein B in the circulating VLDL can reach peripheral lymph without the intermediacy of circulating LDL.

Loss of A-Apoprotein Immunoreactivity during High-Density Lipoprotein Separation

1981 ◽  
Vol 18 (5) ◽  
pp. 308-313 ◽  
Author(s):  
P Johnson ◽  
R A Muirhead ◽  
T Deegan
Keyword(s):  
High Density Lipoprotein ◽  
Surface Structure ◽  
Analytical Ultracentrifugation ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Low Density ◽  
Apoprotein B

By use of an electroimmunoassay, concentrations of A-apoproteins were estimated in serum and in corresponding apoprotein fractions isolated by ultracentrifugation. These values were compared with high-density lipoprotein concentrations determined by analytical ultracentrifugation. Concentrations of A-apoproteins estimated in serum were considerably higher than in isolated high-density lipoprotein fractions. These discrepancies could not be accounted for entirely by material losses into other fractions during ultracentrifugal fractionation. No comparable differences in apoprotein-B concentrations were observed during the ultracentrifugal separation of low-density lipoprotein. Concentrations of A-apoproteins estimated in the residual serum after precipitation of low-density lipoproteins by heparin and manganous ions were also lower than in the corresponding whole sera. The discrepancies persisted after treatment of serum and isolated fractions with tetramethylurea, urea (9 mol/l), and by heating at 52°C for 3 hours. It is considered that separation by ultracentrifugation induces subtle alterations in the surface structure of the lipoprotein species which give rise to changes in immunoreactivity.

Reference intervals for serum lipids, lipoproteins, and apoproteins in the elderly.

1984 ◽  
Vol 30 (3) ◽  
pp. 404-406 ◽  
Author(s):  
C Alvarez ◽  
A Orejas ◽  
S Gonzalez ◽  
R Diaz ◽  
L F Colomo
Keyword(s):  
High Density Lipoprotein ◽  
High Density Lipoprotein Cholesterol ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
High Density ◽  
Lipoprotein Cholesterol ◽  
Low Density ◽  
Low Density Lipoprotein Cholesterol ◽  
Related Difference ◽  
Apoprotein B

Abstract We measured total cholesterol, high-density-lipoprotein cholesterol, low-density-lipoprotein cholesterol, triglycerides, apoprotein A, and apoprotein B in serum. The subjects were a volunteer group of 145 white people (50 men and 95 women) ranging in age between 65 and 95 years, who were not receiving medical treatment and had no disease that could influence lipid metabolism. Before further categorization, we saw no significant sex-related difference in any of these lipid constituents. The mean age of the group was about 80 years, and we compared results for those older and younger. For the women, the only significant difference was a decrease in low-density-lipoprotein cholesterol for those older than 80 years. In men over 80 there was a significant decrease in triglycerides and in apoprotein B and an increase in high-density-lipoprotein cholesterol. The only sex-related difference for persons under and over 80 was in values for high-density-lipoprotein cholesterol, which were higher for men over 80, whereas triglycerides were higher for women over 80.

Studies on the Thromboplastic Effect of Human Plasma Lipoproteins

1976 ◽  
Vol 35 (01) ◽  
pp. 178-185 ◽  
Author(s):  
Helena Sandberg ◽  
Lars-Olov Andersson
Keyword(s):  
High Density Lipoprotein ◽  
Human Plasma ◽  
Platelet Rich Plasma ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Plasma Lipoproteins ◽  
Very Low Density Lipoprotein ◽  
Low Density ◽  
Free Plasma ◽  
Good Agreement

SummaryHuman plasma lipoprotein fractions were prepared by flotation in the ultracentrifuge. Addition of these fractions to platelet-rich, platelet-poor and platelet-free plasma affected the partial thromboplastin and Stypven clotting times to various degrees. Addition of high density lipoprotein (HDL) to platelet-poor and platelet-free plasma shortened both the partial thromboplastin and the Stypven time, whereas addition of low density lipoprotein and very low density lipoprotein (LDL + VLDL) fractions only shortened the Stypven time. The additions had little or no effect in platelet-rich plasma.Experiments involving the addition of anti-HDL antibodies to plasmas with different platelet contents and measuring of clotting times produced results that were in good agreement with those noted when lipoprotein was added. The relation between structure and the clot-promoting activity of various phospholipid components is discussed.

Sign in / Sign up

Export Citation Format

Share Document