Fluorescence studies of protein–sterol relationships in human plasma lipoproteins

Cholesta-5,7,9(11)-trien-3β-ol and its oleate ester were incorporated into human low-density lipoprotein and reconstituted high-density lipoprotein. The unesterified sterol was more efficient than its ester in quenching tryptophan fluorescence, especially in low-density lipoprotein. The results, which indicate that in such lipoproteins unesterified sterols are more closely associated with peptide than are esterified sterols, are used to assess possible structures for the lipoproteins.

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Studies on the Thromboplastic Effect of Human Plasma Lipoproteins

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647942 ◽

1976 ◽

Vol 35 (01) ◽

pp. 178-185 ◽

Cited By ~ 8

Author(s):

Helena Sandberg ◽

Lars-Olov Andersson

Keyword(s):

High Density Lipoprotein ◽

Human Plasma ◽

Platelet Rich Plasma ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

Plasma Lipoproteins ◽

Very Low Density Lipoprotein ◽

Low Density ◽

Free Plasma ◽

Good Agreement

SummaryHuman plasma lipoprotein fractions were prepared by flotation in the ultracentrifuge. Addition of these fractions to platelet-rich, platelet-poor and platelet-free plasma affected the partial thromboplastin and Stypven clotting times to various degrees. Addition of high density lipoprotein (HDL) to platelet-poor and platelet-free plasma shortened both the partial thromboplastin and the Stypven time, whereas addition of low density lipoprotein and very low density lipoprotein (LDL + VLDL) fractions only shortened the Stypven time. The additions had little or no effect in platelet-rich plasma.Experiments involving the addition of anti-HDL antibodies to plasmas with different platelet contents and measuring of clotting times produced results that were in good agreement with those noted when lipoprotein was added. The relation between structure and the clot-promoting activity of various phospholipid components is discussed.

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Smoking and plasma lipoproteins in man: effects on low density lipoprotein cholesterol levels and high density lipoprotein subfraction distribution

European Journal of Clinical Investigation ◽

10.1111/j.1365-2362.1993.tb00724.x ◽

1993 ◽

Vol 23 (10) ◽

pp. 630-640 ◽

Cited By ~ 70

Author(s):

D. J. FREEMAN ◽

B. A. GRIFFIN ◽

E. MURRAY ◽

G. M. LINDSAY ◽

D. GAFFNEY ◽

...

Keyword(s):

High Density Lipoprotein ◽

Low Density Lipoprotein ◽

Density Lipoprotein ◽

High Density ◽

Lipoprotein Cholesterol ◽

Plasma Lipoproteins ◽

Low Density ◽

Low Density Lipoprotein Cholesterol ◽

Cholesterol Levels ◽

Lipoprotein Subfraction

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Observations on the Passage of Apoproteins from Plasma Lipoproteins into Peripheral Lymph in Two Men

Clinical Science ◽

10.1042/cs0490419 ◽

1975 ◽

Vol 49 (5) ◽

pp. 419-426 ◽

Cited By ~ 1

Author(s):

D. Reichl ◽

A. Postiglione ◽

N. B. Myant ◽

J. J. Pflug ◽

M. Press

Keyword(s):

High Density Lipoprotein ◽

Low Density Lipoprotein ◽

Polyacrylamide Gel Electrophoresis ◽

Density Lipoprotein ◽

High Density ◽

Plasma Lipoproteins ◽

Low Density ◽

Peripheral Lymph ◽

Apoprotein B ◽

Plasma High Density

1. The passage of radioactive apolipoproteins into lymph draining the foot was investigated in two men, each given a single intravenous injection of low-density lipoprotein containing 131I-labelled apoprotein B and of very-low-density lipoprotein containing 125I-labelled apoprotein A and apoprotein C. 2. Protein-bound 125I and 131I appeared in the lymph of both subjects. Immunoelectrophoresis of lymph lipoproteins against anti-(high-density lipoprotein) and anti-(low-density lipoprotein) showed the presence of apo-high-density lipoprotein and apo-low-density lipoprotein with faster mobilities than plasma high-density and low-density lipoprotein respectively. Most of the protein-bound 131I in lymph was recovered in the precipitin line formed by the apoprotein B-containing lipoprotein after immunoelectrophoresis. Polyacrylamide gel electrophoresis of the lymph lipoprotein fraction showed the presence of 125I-containing bands with mobilities similar to those of the apoprotein A of high-density lipoprotein and of three of the fast-moving C apoproteins. 3. These results suggest that most, if not all, of the apoproteins of plasma lipoproteins reach the interstitial fluids and that some lipoproteins undergo modification during their passage into peripheral lymph.

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