Comparison between the cellobiose/mannitol and 51Cr-labelled ethylenediaminetetra-acetate absorption tests in the detection of coeliac disease

D. Martines; A. I. Morris; I. T. Gilmore; A. Williams; H. Stockdale; M. Critchley; G. A. Smith; D. Billington

doi:10.1042/cs0750375

Comparison between the cellobiose/mannitol and 51Cr-labelled ethylenediaminetetra-acetate absorption tests in the detection of coeliac disease

Clinical Science ◽

10.1042/cs0750375 ◽

1988 ◽

Vol 75 (4) ◽

pp. 375-378 ◽

Cited By ~ 12

Author(s):

D. Martines ◽

A. I. Morris ◽

I. T. Gilmore ◽

A. Williams ◽

H. Stockdale ◽

...

Keyword(s):

Coeliac Disease ◽

Intestinal Permeability ◽

Absorption Test ◽

51Cr Edta

1. The cellobiose/mannitol and 51Cr-labelled ethylenediaminetetra-acetate (51Cr-EDTA) absorption tests have been compared with respect to their ability to detect altered intestinal permeability in coeliac disease. 2. In patients with clinically proven coeliac disease, 13 out of 14 (93%) had abnormal urinary cellobiose/mannitol ratios, while only five out of 12 (42%) had abnormal urinary recoveries of 51Cr-EDTA. Thus, the cellobiose/mannitol absorption test is more sensitive in recognizing coeliac disease and this difference was significant (P < 0.02). 3. Possible reasons for this difference in the sensitivity of the two absorption tests are discussed.

Download Full-text

51Cr-EDTA/14C-Mannitol Intestinal Permeability Test: Clinical Use in Screening for Coeliac Disease

Scandinavian Journal of Gastroenterology ◽

10.3109/00365528809103963 ◽

1988 ◽

Vol 23 (2) ◽

pp. 171-177 ◽

Cited By ~ 14

Author(s):

K. J. Fotherby ◽

E. P. Wraight ◽

G. Neale

Keyword(s):

Coeliac Disease ◽

Intestinal Permeability ◽

Clinical Use ◽

Permeability Test ◽

51Cr Edta

Download Full-text

A PERSISTENT DEFECT IN INTESTINAL PERMEABILITY IN COELIAC DISEASE DEMONSTRATED BY A 51Cr-LABELLED EDTA ABSORPTION TEST

The Lancet ◽

10.1016/s0140-6736(83)91628-8 ◽

1983 ◽

Vol 321 (8320) ◽

pp. 323-325 ◽

Cited By ~ 190

Author(s):

Ingvar Bjarnason ◽

TimothyJ. Peters ◽

Norman Veall

Keyword(s):

Coeliac Disease ◽

Intestinal Permeability ◽

Absorption Test

Download Full-text

Intestinal Permeability to 51Cr-EDTA and Orocecal Transit Time in Cystic Fibrosis

Journal of Pediatric Gastroenterology and Nutrition ◽

10.1097/00005176-199202000-00015 ◽

1992 ◽

Vol 14 (2) ◽

pp. 204-207 ◽

Cited By ~ 24

Author(s):

H. Escobar ◽

M. Perdomo ◽

F. Vasconez ◽

C. Camarero ◽

M. T. del Olmo ◽

...

Keyword(s):

Cystic Fibrosis ◽

Intestinal Permeability ◽

Transit Time ◽

Orocecal Transit Time ◽

51Cr Edta ◽

Orocecal Transit

Download Full-text

Intestinal permeability in allergic rats: nerve involvement in antigen-induced changes

AJP Gastrointestinal and Liver Physiology ◽

10.1152/ajpgi.1993.264.4.g617 ◽

1993 ◽

Vol 264 (4) ◽

pp. G617-G623 ◽

Cited By ~ 7

Author(s):

S. E. Crowe ◽

K. Soda ◽

A. M. Stanisz ◽

M. H. Perdue

Keyword(s):

Intestinal Permeability ◽

Fold Increase ◽

Serum Levels ◽

Baseline Period ◽

Antigen Challenge ◽

51Cr Edta ◽

Neural Factors ◽

Induced Changes ◽

In Vivo Uptake

In vivo uptake of the probe 51Cr-labeled EDTA from the jejunum of egg albumin (EA)-sensitized rats was compared with controls at baseline and after intraluminal antigen challenge. Probe recovery in blood was 60-80% greater in sensitized animals during the baseline period, suggesting that sensitization resulted in increased intestinal permeability. Sensitized, but not control, rats demonstrated a 15-fold increase in 51Cr-EDTA uptake after intraluminal antigen; no change occurred with an unrelated protein. Macromolecular recovery was also enhanced in sensitized animals, since serum levels of immunoreactive EA were elevated 14-fold compared with controls. Antigen challenge was accompanied by biochemical (protease release) and morphological (reduced numbers) evidence of mast cell degranulation in sensitized rats. The neurotoxin tetrodotoxin (applied directly to ligated jejunal segments) inhibited EA-induced uptake of 51Cr-EDTA and antigen. In isolated jejunum from sensitized rats, tetrodotoxin reduced secretory responses to luminal, but not serosal, antigen. These results indicate that neural factors may influence the uptake of molecules from the gut lumen during intestinal anaphylaxis.

Download Full-text