Serum creatine kinase MM isoforms in hypertrophic cardiomyopathy

1991 ◽  
Vol 81 (6) ◽  
pp. 723-726 ◽  
Author(s):  
Mareomi Hamada ◽  
Takashi Ohtani ◽  
Michihito Sekiya ◽  
Yasushi Fujiwara ◽  
Takumi Sumimoto ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Hypertrophic Cardiomyopathy ◽  
Normal Control ◽  
Activity Ratio ◽  
Serum Creatine Kinase ◽  
Myocardial Tissue ◽  
Control Subjects ◽  
Creatine Kinase Mb ◽  
Total Creatine

1. To determine whether a persistent release of creatine kinase from the myocardium occurs in patients with hypertrophic cardiomyopathy, the activities of serum creatine kinase MM isoforms were measured in 22 patients with hypertrophic cardiomyopathy and in 14 normal control subjects. 2. Serum creatine kinase MB activity was significantly higher in patients with hypertrophic cardiomyopathy (7.8 ± 3.8 i.u./l) than in normal control subjects (0.4 ± 0.8 i.u./l; P < 0.01). 3. Serum MMa, MMb and MMc activities in patients with hypertrophic cardiomyopathy were 19.4 ± 4.1%, 26.7 ± 2.5% and 33.5 ± 7.0% of the total creatine kinase MM activity, respectively. These values for each isoform were significantly different from those in normal control subjects (11.3 ± 3.0%, 21.5 ± 4.4% and 40.7 ± 7.0%, respectively). The MMa/MMc activity ratio was significantly higher in patients with hypertrophic cardiomyopathy (0.61 ± 0.25) than in normal control subjects (0.30 ± 0.10; P < 0.01). 4. Our results indicate that a small amount of the myocardial tissue isoform of creatine kinase MM (MMa) is constantly released in many patients with hypertrophic cardiomyopathy.

Total Creatine Kinase and Isoenzyme Fractions in Chronic Dialysis Patients

1987 ◽  
Vol 10 (2) ◽  
pp. 72-76 ◽  
Author(s):  
S.M. Lal ◽  
K.D. Nolph ◽  
H. Hain ◽  
H.L. Moore ◽  
R. Khanna ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Serum Creatine Kinase ◽  
Chronic Dialysis ◽  
Maintenance Hemodialysis ◽  
Total Serum ◽  
Dialysis Patients ◽  
Creatine Kinase Mb ◽  
Total Creatine

We measured total serum creatine kinase (CK) and serum creatine kinase MB fraction (CK-MB) in 53 patients on continuous ambulatory peritoneal dialysis (CAPD) and 52 patients on maintenance hemodialysis (HD), using Scalva UV methodology for CK and electrophoresis for CK-MB. Seven of the 53 CAPD patients (13%) had an elevated total CK, and only one of these 7 patients had an elevated CK-MB > 5%. In contrast 22 HD patients (42%) had increased total CK values, and 6 of these 22 HD patients (27%) showed elevated CK-MB isoenzyme > 5%. For each sex, blacks had higher mean CK values than whites. Twenty-one out of the 43 HD patients who received intramuscular injections had elevated total CK values and 6 of these 21 patients had elevated CK-MB isoenzyme independent of the timing of injection. The increased frequency of higher total CK values in HD patients appears to be related to race and androgen administration. The modest elevations in CK-MB fraction (5 to 8%) in these patients require careful interpretation.

ASSOCIATION OF SERUM LACTATE LEVELS WITH CREATINE KINASE – MB AND LACTATE DEHYDROGENASE IN PATIENTS WITH MYOCARDIAL INFARCTION

2021 ◽  
pp. 73-75
Author(s):  
Mallaiyan Manonmani ◽  
Meiyappan Kavitha
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Serum Lactate ◽  
Serum Creatine Kinase ◽  
Hemodynamic Management ◽  
Diagnosis And Prognosis ◽  
Creatine Kinase Mb ◽  
Enzymatic Markers ◽  
Lactate Levels

Objectives: Myocardial infarction is the most common form of coronary heart disease, the commonest cause of worldwide mortality. The present biochemical markers take atleast 6 hours for elevation following an episode of myocardial infarction. There is a need for sensitive marker for early diagnosis and prognosis. Lactate, the end product of anaerobic glycolysis is found to be elevated in many critical illnesses. Thus the study was undertaken to assess the levels of serum lactate in patients with myocardial infarction and to correlate it with the frequently used enzymatic markers for the diagnosis of myocardial infarction, i.e creatine kinase – MB and lactate dehydrogenase Methods: Fifty age and sex matched controls and fty cases of myocardial infarction were included in the study. Serum creatine kinase – MB, lactate dehydrogenase and lactate were estimated in these subjects. Results:The serum lactate levels were signicantly higher among cases when compared to controls. The serum lactate levels positively correlated with serum creatine kinase – MB among cases but not with lactate dehydrogenase. Conclusions: We conclude that serum lactate is altered in patients with myocardial infarction and may be considered as a prognostic risk factor in these patients. Further studies are needed to nd the cut-off value of serum lactate for assistance in the hemodynamic management of these patients.

Further heterogeneity demonstrated for serum creatine kinase isoenzyme MM.

1980 ◽  
Vol 26 (3) ◽  
pp. 457-462 ◽  
Author(s):  
J P Chapelle ◽  
C Heusghem
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Electrophoretic Pattern ◽  
Coronary Bypass ◽  
Blood Stream ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Major Band ◽  
Normal Human ◽  
Total Creatine

Abstract Serum creatine kinase (EC 2.1.3.2) isoenzyme MM was resolved by isoelectric focusing into a five-band pattern, a pattern that gradually changed after the onset of myocardial infarction. Similar changes were also demonstrated in patients undergoing coronary-bypass surgery. The evolution of two CK-MB sub-bands was studied in both cases. We found that three electrophoretic bands (CK-MM, pI 7.10; MM1, pI 6.88; MB1, pI 5.61) were predominant in patterns for sera collected during the early phase of myocardial infarction, but rapidly disappeared during the following hours, whereas bands of increased electrophoretic mobility (MM2, pI 6.70; MM3, pI 6.45; MM4, pI 6.25; MB2, pI 5.34) gradually increased. MM3 was always the major band at the end of the observation period in acute myocardial infarction (mean, 61.4% of total creatine kinase activity 36 h after the peak value for total creatine kinase in serum). The CK-MM bands were also present in the serum of patients without heart disease. Changes in the electrophoretic pattern were induced by a thermolabile factor in normal human serum, which transformed the muscular or myocardial MM and MM1 bands after their release into the blood stream.

Ratio of Creatine Kinase 2 Mass Concentration to Total Creatine Kinase Activity not Altered by Heavy Physical Exercise

1992 ◽  
Vol 38 (11) ◽  
pp. 2224-2227 ◽  
Author(s):  
J Ordóñez-Llanos ◽  
J R Serra-Grima ◽  
J Mercé-Muntañola ◽  
F González-Sastre
Keyword(s):  
Creatine Kinase ◽  
Mass Concentration ◽  
Kinase Activity ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Marathon Runners ◽  
Creatine Kinase Isoenzyme ◽  
Total Creatine Kinase Activity ◽  
Total Creatine ◽  
Sedentary Subjects

Abstract Serum creatine kinase isoenzyme 2 concentrations (CK 2 mass) were measured in marathon runners during training and 1 and 2 days after a race and compared with values from 36 acute myocardial infarction (AMI) patients whose total CK and (or) CK 2 activities were similar to those of runners in the basal state. During training, runners had CK and CK 2 activities 53% and 43% above reference values, respectively, and 36% had CK 2 activity &gt; 5% of total CK. Nine runners (26%) showed CK 2 mass values &gt; 6 micrograms/L but &lt; or = 10 micrograms/L; 35 of the AMI subjects, despite having CK activities similar to those of runners, had values &gt; 10 micrograms/L. The ratio of CK 2 mass to total CK activity was significantly (P &lt; 0.0002) different between sexes for runners. At 1 and 2 days after racing, 100% of CK and CK 2 activities and 71% and 57% of the percentages of CK 2 activity, respectively, were abnormally high; 57% and 43% of CK 2 mass values were &gt; 10 micrograms/L, being comparable with those observed for the AMI group. Basal CK 2 mass values of the runners appeared only slightly higher than that for sedentary subjects, but after exercise half the subjects presented increased values similar to those observed for AMI subjects. The ratio of CK 2 mass to total CK activity appeared unaltered by exercise in all but one of the samples assayed, indicating its utility in evaluating CK 2 mass increases originating in skeletal muscle.

Serum Enzymes and Isoenzymes after Surgery

1977 ◽  
Vol 14 (1-6) ◽  
pp. 294-296 ◽  
Author(s):  
J. Krafft ◽  
R. Fink ◽  
S. B. Rosalki
Keyword(s):  
Myocardial Infarction ◽  
Creatine Kinase ◽  
Lactate Dehydrogenase ◽  
Clinical Evidence ◽  
Serum Creatine Kinase ◽  
Serum Enzymes ◽  
Aspartate Transaminase ◽  
Rare Association ◽  
Creatine Kinase Mb

Serum creatine kinase, aspartate transaminase, and hydroxybutyrate dehydrogenase activities were abnormal in 76, 50, and 28% respectively of 50 patients studied within 26 hours of surgery. No patient showed clinical evidence of myocardial infarction. Creatine kinase MB isoenzyme elevation, and lactate dehydrogenase LD1 activity greater than LD2 (LD1 > LD2) were infrequent (6 and 10% respectively). No patient showed the combination of transient MB isoenzyme elevation and LD1 > LD2, although their rare association without infarction after surgery is to be anticipated.

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