Glomerular Expression of Smooth-Muscle Myosin Heavy-Chain Isoforms in Aminonucleoside Nephrosis in Rats

1995 ◽  
Vol 89 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Tsukasa Nakamura ◽  
Kenjiro Kimura ◽  
Isao Ebihara ◽  
Toshimasa Takahashi ◽  
Yasuhiko Tomino ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Proliferating Cell Nuclear Antigen ◽  
Smooth Muscle Actin ◽  
Nuclear Antigen ◽  
Puromycin Aminonucleoside ◽  
Proliferating Cell ◽  
Aminonucleoside Nephrosis ◽  
Puromycin Aminonucleoside Nephrosis

1. We investigated the glomerular expression of three types of myosin heavy-chain isoforms, including S-myosin heavy-chain 40 (SM1), S-myosin heavy-chain 29 (SM2) and FS-myosin heavy-chain 34 (SMemb) in puromycin aminonucleoside nephrosis. 2. There was little change in SM1 and SM2 mRNA levels throughout the experiment. In contrast, glomerular SMemb mRNA increased on days 2 and 4 (before and soon after the onset of proteinuria, respectively), but declined on day 8 (the peak of proteinuria). 3. Histological myosin heavy-chain expression was examined using three antibodies against SM1, SM2 and SMemb. Immunohistochemically, SM1 and SM2 were absent in the glomeruli associated with puromycin aminonucleoside nephrosis until day 20. The SMemb isoform was barely detectable in normal glomeruli, but substantial amounts of SMemb were demonstrated in the glomeruli of rats with puromycin aminonucleoside nephrosis. In the puromycin aminonucleoside-treated rats, the number of SMemb-positive glomerular cells increased on days 2 and 4. 4. We examined whether levels of α-smooth-muscle actin or proliferating cell nuclear antigen correlated with myosin heavy-chain levels in the glomeruli of rats with puromycin aminonucleoside nephrosis. None of the cellular components in the glomeruli was positive for either α-smooth-muscle actin or proliferating cell nuclear antigen in puromycin aminonucleoside nephrosis. 5. Administration of methylprednisolone to puromycin aminonucleoside-treated rats resulted in the rapid disappearance of proteinuria. However, methylprednisolone did not affect SMemb mRNA or immunostaining in the glomeruli of rats with puromycin aminonucleoside nephrosis. 6. These data suggest that SMemb may be a molecular marker for phenotypic change in early glomerular injury, and demonstrate that SMemb regulation differs from that of SM1, SM2, α-smooth-muscle actin and proliferating cell nuclear antigen in the glomeruli of rats with puromycin aminonucleoside nephrosis.

Expression of Desmin and Smooth Muscle Myosin Heavy Chain in Dermatofibromas

2002 ◽  
Vol 126 (10) ◽  
pp. 1179-1183 ◽  
Author(s):  
Andrea K. Bruecks ◽  
Martin J. Trotter
Keyword(s):  
Smooth Muscle ◽  
Myosin Heavy Chain ◽  
Heavy Chain ◽  
Smooth Muscle Actin ◽  
Smooth Muscle Myosin ◽  
Immunoperoxidase Staining ◽  
Muscle Actin ◽  
Muscle Myosin ◽  
Hematoxylin Eosin ◽  
Focal Expression

Abstract Background.—The histopathologic features of dermatofibroma vary remarkably, and this diversity may occasionally cause problems in differentiating between benign and malignant mesenchymal lesions, including smooth muscle neoplasms. Immunohistochemical stains are sometimes necessary to clarify the histogenesis of a lesion. Objective.—To evaluate dermatofibromas for expression of desmin and smooth muscle myosin heavy chain (SM-MHC) antigens, which are commonly used as evidence of smooth muscle differentiation. Methods.—We studied 100 consecutive cases of dermatofibroma using hematoxylin-eosin–stained sections and immunoperoxidase staining with antibodies against desmin, SM-MHC, and smooth muscle actin. Results.—We found focal positivity for desmin in 9 cases, and in 2 of these cases, at least 10% of lesional cells showed strong expression. We found focal staining for SM-MHC in 10 cases, and in 2 of these cases, at least 10% of the lesional cells were positive. Regions positive for desmin and/or SM-MHC did not show definite histologic features of myogenous differentiation on hematoxylin-eosin–stained sections. All dermatofibromas expressing desmin and SM-MHC were also strongly positive for smooth muscle actin. Conclusions.—About 10% of dermatofibromas show focal expression of desmin and SM-MHC, and this expression may be present in up to 10% to 15% of lesional cells. Thus, in dermal spindle cell lesions, focal expression of these muscle antigens, like that of smooth muscle actin, is not diagnostic of a smooth muscle tumor.

scholarly journals Proliferating cell nuclear antigen (PCNA) expressed in human leptomeninges.

1996 ◽  
Vol 44 (11) ◽  
pp. 1261-1265 ◽  
Author(s):  
H Funato ◽  
M Yoshimura ◽  
Y Ito ◽  
R Okeda ◽  
Y Ihara
Keyword(s):  
Monoclonal Antibody ◽  
Cell Proliferation ◽  
Smooth Muscle ◽  
Proliferating Cell Nuclear Antigen ◽  
Normal Subjects ◽  
Nuclear Antigen ◽  
Ependymal Cells ◽  
Ki 67 ◽  
Proliferating Cell ◽  
Strong Immunoreactivity

Here we report on the presence of proliferating cell nuclear antigen (PCNA) in human leptomeninges from 35 normal subjects with ages ranging from 57 to 94 years. Strong immunoreactivity with PC10 (a monoclonal antibody to PCNA) was detected in the nuclei of meningothelial cells, smooth muscle cells of leptomeningeal vessels, and ependymal cells. An immunoblot of leptomeningeal homogenate with PC10 showed the presence of a single band at 35 KD, the expected molecular mass of PCNA. Ki-67, another marker for cell proliferation, was undetectable in human leptomeninges. These observations point to isolated PCNA expression in tissue in which cells are not actively proliferating.

scholarly journals Retrobulbar Fibrosarcoma in a Sheep

2016 ◽  
Vol 66 (2) ◽  
pp. 265-270 ◽  
Author(s):  
Ozlem Ozmen ◽  
Ozlem Sirin Sengoz ◽  
Harun Çinar ◽  
Hüseyin Dolu
Keyword(s):  
Proliferating Cell Nuclear Antigen ◽  
Smooth Muscle Actin ◽  
S100 Protein ◽  
Surrounding Tissue ◽  
Nuclear Antigen ◽  
Collagenous Matrix ◽  
Proliferating Cell ◽  
Cut Surface ◽  
Female Sheep ◽  
Pleomorphic Cells

Abstract This report describes a case of a retrobulbar fibrosarcoma in a 4-year-old female sheep. A big tumor was protruding from the orbit but not invading the surrounding tissue and was easily removed surgically. The grayish colored mass was 19×13×8 cm in size and hard. The surface of the tumor was irregular and ulcerated. Marked hemorrhage and necrotic areas were present across the whitish cut surface. Histopathologically, the mass was composed of spindle shaped, anaplastic pleomorphic cells. Masson’s trichrome staining revealed a collagenous matrix in the tissue. Immunohistochemically, the mass was positive for vimentin and proliferating cell nuclear antigen but negative for smooth muscle actin, desmin, glial fibrilar acidic protein and S100 protein antibodies. According to histopathological and immunohistochemical findings the tumor was diagnosed as fibrosarcoma. To the authors’ knowledge, this is the first report of ocular fibrosarcoma in a sheep.

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