Phenotyping of epidermal dendritic cells allows the differentiation between extrinsic and intrinsic forms of atopic dermatitis

2000 ◽  
Vol 143 (6) ◽  
pp. 1193-1198 ◽  
Author(s):  
T. Oppel ◽  
E. Schuller ◽  
S. Günther ◽  
M. Moderer ◽  
J. Haberstok ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Atopic Dermatitis ◽  
Epidermal Dendritic Cells

Evidence of regulatory myeloid dendritic cells and circulating inflammatory epidermal dendritic cells-like modulated by Toll-like receptors 2 and 7/8 in adults with atopic dermatitis

2017 ◽  
Vol 56 (6) ◽  
pp. 630-635 ◽  
Author(s):  
Vanessa G. dos Santos ◽  
Raquel L. Orfali ◽  
Tiago de Oliveira Titz ◽  
Alberto J. da Silva Duarte ◽  
Maria N. Sato ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Atopic Dermatitis ◽  
Toll Like Receptors ◽  
Myeloid Dendritic Cells ◽  
Epidermal Dendritic Cells

scholarly journals Immunohistopathological Analysis of Immunoglobulin E-Positive Epidermal Dendritic Cells with House Dust Mite Antigens in Naturally Occurring Skin Lesions of Adult and Elderly Patients with Atopic Dermatitis

2021 ◽  
Vol 8 (3) ◽  
pp. 426-441
Author(s):  
Ryoji Tanei ◽  
Yasuko Hasegawa
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Atopic Dermatitis ◽  
House Dust ◽  
House Dust Mite ◽  
Immunoglobulin E ◽  
Skin Lesions ◽  
Delayed Type Hypersensitivity ◽  
Dust Mite ◽  
Epidermal Dendritic Cells

The immunopathogenic role of house dust mite (HDM) allergens in the development of skin lesions in atopic dermatitis (AD) has not yet been precisely clarified. We immunohistopathologically evaluated the localization of immunoglobulin E (IgE)-positive epidermal dendritic cells with HDM antigens in the skin lesions of patients with IgE-allergic AD. Using double-immunofluorescence and single-immunochemical staining methods, we analyzed biopsy specimens from the skin lesions of six patients with IgE-allergic AD and HDM allergy and 11 control subjects with inflammatory skin disorders. Inflammatory dendritic epidermal cells (IDECs; CD11c+ and CD206+ cells) were markedly observed in the central area of the spongiotic epidermis of skin lesions in all AD patients. Furthermore, IgE-positive IDECs with HDM antigens in the central areas of the spongiosis were found in four of the six (66.7%) AD patients. Langerhans cells (LCs; CD207+ cells) with HDM antigens were also observed in the peripheral areas of the spongiosis. Infiltration of CD4+ and CD8+ T cells in association with IgE-positive IDECs and LCs with HDM antigens was seen in the spongiotic epidermis. An IgE-mediated delayed-type hypersensitivity reaction, in combination with IgE-bearing dendritic cells, specific T cells, keratinocytes, and HDM antigens, may lead to spongiotic tissue formation in eczematous dermatitis in AD.

scholarly journals Molecular Mechanisms Involved in the Migration of Epidermal Dendritic Cells in the Skin

1999 ◽  
Vol 4 (2) ◽  
pp. 169-172 ◽  
Author(s):  
Koichiro Nakamura ◽  
Kunihiko Tamaki ◽  
Atsushi Saitoh ◽  
Nami Yasaka ◽  
Masutaka Furue
Keyword(s):  
Dendritic Cells ◽  
Molecular Mechanisms ◽  
Epidermal Dendritic Cells

scholarly journals HIV-1 Selection by Epidermal Dendritic Cells during Transmission across Human Skin

1998 ◽  
Vol 187 (10) ◽  
pp. 1623-1631 ◽  
Author(s):  
Jeanette C. Reece ◽  
Amanda J. Handley ◽  
E. John Anstee ◽  
Wayne A. Morrison ◽  
Suzanne M. Crowe ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
Human Skin ◽  
Cell Sorting ◽  
Virus Entry ◽  
Skin Explants ◽  
Skin Uptake ◽  
Virus Exposure ◽  
Epidermal Dendritic Cells ◽  
Hiv 1

Macrophage tropic HIV-1 is predominant during the initial viremia after person to person transmission of HIV-1 (Zhu, T., H. Mo, N. Wang, D.S. Nam, Y. Cao, R.A. Koup, and D.D. Ho. 1993. Science. 261:1179–1181.), and this selection may occur during virus entry and carriage to the lymphoid tissue. Human skin explants were used to model HIV-1 selection that may occur at the skin or mucosal surface. Macrophage tropic, but not T cell line tropic strains of HIV-1 applied to the abraded epidermis were recovered from the cells emigrating from the skin explants. Dermis and epidermis were separated by dispase digestion after virus exposure to determine the site of viral selection within the skin. Uptake and transmission to T cells of all HIV-1 isolates was found with the dermal emigrant cells, but only macrophage tropic virus was transferred by emigrants from the epidermis exposed to HIV-1, indicating selection only within the epidermis. CD3+, CD4+ T cells were found in both the dermal and epidermal emigrant cells. After cell sorting to exclude contaminating T cells, macrophage tropic HIV-1 was found in both the dermal emigrant dendritic cells and in dendritic cells sorted from the epidermal emigrants. These observations suggest that selective infection of the immature epidermal dendritic cells represents the cellular mechanism that limits the initial viremia to HIV-1 that can use the CCR5 coreceptor.

The presence of IgE on macrophages and dendritic cells infiltrating into the skin lesion of atopic dermatitis

1987 ◽  
Vol 42 (3) ◽  
pp. 328-337 ◽  
Author(s):  
Donald Y.M. Leung ◽  
Eveline E. Schneeberger ◽  
Reuben P. Siraganian ◽  
Raif S. Geha ◽  
Atul K. Bhan
Keyword(s):  
Dendritic Cells ◽  
Atopic Dermatitis ◽  
Skin Lesion

scholarly journals Pseudomelanoma diagnostic through confocal microscopy: the challenge of the epidermal dendritic cells

2015 ◽  
Vol 7 (3) ◽  
Author(s):  
Fernanda Berti Rocha Mendes ◽  
Raquel de Paula Ramos Castro ◽  
Mariana Petaccia de Macedo ◽  
Clovis Antonio Lopes Pinto ◽  
João Pedreira Duprat Neto ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Confocal Microscopy ◽  
Epidermal Dendritic Cells

scholarly journals Congenital Deficiency of Conventional Dendritic Cells Promotes the Development of Atopic Dermatitis-Like Inflammation

2021 ◽  
Vol 12 ◽  
Author(s):  
Yotaro Nishikawa ◽  
Tomohiro Fukaya ◽  
Takehito Fukui ◽  
Tomofumi Uto ◽  
Hideaki Takagi ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Atopic Dermatitis ◽  
Immune Responses ◽  
Skin Barrier ◽  
Lymphoid Cells ◽  
Congenital Deficiency ◽  
T Cell Immune Responses ◽  
Group 2 ◽  
And Function ◽  
The Impact

Atopic dermatitis (AD) is a common pruritic inflammatory skin disease characterized by impaired epidermal barrier function and dysregulation of Thelper-2 (TH2)-biased immune responses. While the lineage of conventional dendritic cells (cDCs) are implicated to play decisive roles in T-cell immune responses, their requirement for the development of AD remains elusive. Here, we describe the impact of the constitutive loss of cDCs on the progression of AD-like inflammation by using binary transgenic (Tg) mice that constitutively lacked CD11chi cDCs. Unexpectedly, the congenital deficiency of cDCs not only exacerbates the pathogenesis of AD-like inflammation but also elicits immune abnormalities with the increased composition and function of granulocytes and group 2 innate lymphoid cells (ILC2) as well as B cells possibly mediated through the breakdown of the Fms-related tyrosine kinase 3 ligand (Flt3L)-mediated homeostatic feedback loop. Furthermore, the constitutive loss of cDCs accelerates skin colonization of Staphylococcus aureus (S. aureus), that associated with disease flare. Thus, cDCs maintains immune homeostasis to prevent the occurrence of immune abnormalities to maintain the functional skin barrier for mitigating AD flare.

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