Extracellular Sodium Dependence of GnRH‐Stimulated Growth Hormone Release in Goldfish Pituitary Cells

1997 ◽  
Vol 9 (3) ◽  
pp. 207-216 ◽  
Author(s):  
Fredrick Van Goor ◽  
Jeffrey I. Goldberg ◽  
John P. Chang
Keyword(s):  
Growth Hormone ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Sodium Dependence ◽  
Extracellular Sodium

Cytokine-Mediated Growth Hormone Release from Cultured Ovine Pituitary Cells

1998 ◽  
Vol 68 (3) ◽  
pp. 192-200 ◽  
Author(s):  
Christa L. Fry ◽  
David R. Gunter ◽  
Christopher D. McMahon ◽  
Barbara Steele ◽  
James L. Sartin
Keyword(s):  
Growth Hormone ◽  
Pituitary Cells ◽  
Hormone Release ◽  
Growth Hormone Release

GRF increases release of growth hormone and arachidonate from anterior pituitary cells

1985 ◽  
Vol 248 (4) ◽  
pp. E438-E442
Author(s):  
A. M. Judd ◽  
K. Koike ◽  
R. M. MacLeod
Keyword(s):  
Growth Hormone ◽  
Anterior Pituitary ◽  
Pituitary Cells ◽  
Dependent Manner ◽  
Hormone Release ◽  
Growth Hormone Release ◽  
Concentration Dependent Manner ◽  
Anterior Pituitary Cells ◽  
Arachidonate Release

Arachidonate and its metabolites increase growth hormone release in vitro. A study was designed to determine whether arachidonate release from anterior pituitary cells is modified by growth hormone-releasing factor (GRF) or somatostatin (SRIF). Cultured pituitary cells were incubated with [3H]arachidonate to esterify the long-chain fatty acid into cellular lipids. The cells were extensively washed with medium containing no [3H]arachidonate and then incubated with GRF and/or SRIF for 30 min. The incubation medium was then extracted with ethyl acetate, and following thin-layer chromatographic separation, the radioactivity in the [3H]arachidonate band was measured. GRF in a concentration-dependent manner (1-30 nM) stimulated growth hormone and arachidonate release, whereas SRIF (100 nM) blocked the GRF-induced increase of growth hormone and arachidonate release. The effects of GRF on growth hormone and arachidonate were evident at time intervals as brief as 5 min. These findings support the hypothesis that arachidonate may play a role in the GRF-induced growth hormone release.

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