Localisation and Semi-Quantitative Measurement Of Lipocortin 1 In Rat Anterior Pituitary Cells By Fluorescence-Activated Cell Analysis/Sorting and Electron Microscopy

2001 ◽  
Vol 11 (9) ◽  
pp. 707-714 ◽  
Author(s):  
Christian ◽  
Flower ◽  
Morris ◽  
Buckingham
Keyword(s):  
Electron Microscopy ◽  
Anterior Pituitary ◽  
Quantitative Measurement ◽  
Pituitary Cells ◽  
Cell Analysis ◽  
Anterior Pituitary Cells

Dopamine enters lactotrophs and reaches their secretory granules

1985 ◽  
Vol 104 (1) ◽  
pp. 23-NP ◽  
Author(s):  
M. G. P. Gallardo ◽  
M. Bilinski ◽  
S. R. Chiocchio ◽  
J. H. Tramezzani
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Anterior Pituitary ◽  
Secretory Granules ◽  
Pituitary Cells ◽  
Histochemical Reaction ◽  
Anterior Pituitary Cells ◽  
Biochemical Assays ◽  
Pituitary Lactotroph

ABSTRACT The presence of dopamine in the lactotroph cell, as well as in isolated prolactin secretory granules, was demonstrated by means of an histochemical reaction for electron microscopy. Biochemical assays further confirmed the presence of dopamine in the secretory granules. Autoradiographic preparations examined by light microscopy showed dopamine internalization in dispersed anterior pituitary cells. Isolated anterior pituitary lactotroph cells incorporated more [3H]dopamine than a fraction containing other anterior pituitary cells. J. Endocr. (1985) 104, 23–28

scholarly journals Visualization of the Nuclear Lamina in Mouse Anterior Pituitary Cells and Immunocytochemical Detection of Lamin A/C by Quick-freeze Freeze-substitution Electron Microscopy

2005 ◽  
Vol 53 (4) ◽  
pp. 497-507 ◽  
Author(s):  
Takao Senda ◽  
Akiko Iizuka-Kogo ◽  
Atsushi Shimomura
Keyword(s):  
Electron Microscopy ◽  
Nuclear Membrane ◽  
Anterior Pituitary ◽  
Freeze Substitution ◽  
Nuclear Lamina ◽  
Immunogold Electron Microscopy ◽  
Lamin A ◽  
Pituitary Cells ◽  
Inner Nuclear Membrane ◽  
Anterior Pituitary Cells

We examined the nuclear lamina in the quickly frozen anterior pituitary cells by electron microscopic techniques combined with freeze substitution, deep etching, and immunocytochemistry and compared it with that in the chemically fixed cells. By quick-freeze freeze-substitution electron microscopy, an electron-lucent layer, as thick as 20 nm, was revealed just inside the inner nuclear membrane, whereas in the conventionally glutaraldehyde-fixed cells the layer was not seen. By quick-freeze deep-etch electron microscopy, we could not distinguish definitively the layer corresponding to the nuclear lamina in either fresh unfixed or glutaraldehyde-fixed cells. Immunofluorescence microscopy showed that lamin A/C in the nucleus was detected in the acetone-fixed cells and briefly in paraformaldehyde-fixed cells but not in the cells with prolonged paraformaldehyde fixation. Nuclear localization of lamin A/C was revealed by immunogold electron microscopy also in the quickly frozen and freeze-substituted cells, but not in the paraformaldehyde-fixed cells. Lamin A/C was localized mainly in the peripheral nucleoplasm within 60 nm from the inner nuclear membrane, which corresponded to the nuclear lamina. These results suggest that the nuclear lamina can be preserved both ultrastructurally and immunocytochemically by quick-freezing fixation, rather than by conventional chemical fixation.

Surface morphology and secretory activity of rat anterior pituitary cells in primary culture

1978 ◽  
Vol 36 (2) ◽  
pp. 584-585
Author(s):  
T. Antakly ◽  
F. Zeytinoglu ◽  
G. Pelletier ◽  
F. Labrie
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Morphology ◽  
Primary Culture ◽  
Anterior Pituitary ◽  
Secretory Activity ◽  
Secretory Cells ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Scanning Electron

So far, there has been no report concerning the surface morphology of cultured secretory cells in different states of activity, as observed by scanning electron microscopy. The anterior pituitary cells in monolayer culture offer an unique system to study the modifications of cell conformation in relation with changes of activity since these cells can be specifically modulated by stimulating or inhibiting factors. Scanning electron microscopy of anterior pituitary cells in primary culture was thus performed in different states of secretory activity. Adult female Sprague-Dawley rats at random stage of the estrous cycle, were used for the preparation of the primary cultures of anterior pituitary cells as previously described (Endocrinology 98: 1528, 1976). The cells 7. 5 x 105 in 1. 5 ml of Dulbecco's modified Eagle's medium containing 10% horse serum and 0. 25% foetal calf serum were plated in 3. 5 x 10 mm Petri dishes and were used six days after plating.

scholarly journals A study of acid phosphatase and dipeptidyl aminopeptidase II in monodispersed anterior pituitary cells using flow cytometry and electron microscopy.

1979 ◽  
Vol 27 (11) ◽  
pp. 1499-1504 ◽  
Author(s):  
R E Smith ◽  
P N Dean
Keyword(s):  
Electron Microscopy ◽  
Flow Cytometry ◽  
Acid Phosphatase ◽  
Anterior Pituitary ◽  
Single Cells ◽  
Fluorescent Microscopy ◽  
Historical Review ◽  
Pituitary Cells ◽  
Anterior Pituitary Cells ◽  
Dipeptidyl Aminopeptidase

A brief historical review of cytoenzymology is presented from the time of introduction into electron microscopy to the present, where the direction for quantification of an enzyme in single cells appears most promising by fluorescent staining. First attempts are reported to quantitate acid phosphatase (AcPase) and dipeptidyl aminopeptidase II (DAP-II) in monodispersed anterior pituitary cells from lactating and postlactating rats by flow cytometry, fluorescent, and electron microscopy. 3-Hydroxy-flavone is introduced as a new fluorescent cytochemical stain for AcPase, useful in flow cytometry but of only limited use in fluorescent microscopy. Histograms for AcPase indicate a single peak of cells staining more intensely in cell preparations from postlactating over lactating animals. Histograms for DAP-II staining indicate two distinct populations of cells present in the lactating and only one in the postlactating rat anterior pituitary gland. The application of dual laser staining indicates that not all cells stain for both enzymes. Electron microscopy shows the subcellular localization of DAP-II to be limited to lytic bodies and in mammotrophic cells to some secretion granules.

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