scholarly journals Biological and sequence comparisons of Potato virus Y isolates associated with potato tuber necrotic ringspot disease

2002 ◽  
Vol 51 (2) ◽  
pp. 117-126 ◽  
Author(s):  
N. Boonham ◽  
K. Walsh ◽  
M. Hims ◽  
S. Preston ◽  
J. North ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Sequence Comparisons

scholarly journals Occurrence in Tunisia of potato tuber necrotic ringspot disease (PTNRD) caused by variant PVYNTNof Potato virus Y

2008 ◽  
Vol 57 (2) ◽  
pp. 388-388 ◽  
Author(s):  
S. Boukhris Bouhachem ◽  
N. Khamassy ◽  
L. Glais ◽  
C. Kerlan
Keyword(s):  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y

scholarly journals Occurrence of Diverse Recombinant Strains of Potato virus Y Circulating in Potato Fields in Egypt

Plant Disease ◽  
2017 ◽  
Vol 101 (8) ◽  
pp. 1463-1469 ◽  
Author(s):  
Esraa A. Elwan ◽  
Engy E. Abdel Aleem ◽  
Faiza A. Fattouh ◽  
Kelsie J. Green ◽  
Lisa T. Tran ◽  
...  
Keyword(s):  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Molecular Properties ◽  
Whole Genome ◽  
The Novel ◽  
Limited Information ◽  
Genome Sequences ◽  
Recombinant Strains ◽  
Virus Strains

Potato is one of the staple crops in Egypt, grown under irrigation almost continuously year-round. Potato virus Y (PVY) has been reported as one of the main viruses affecting potatoes in Egypt, but limited information is available on PVY strains circulating in potato fields in the country. From 2014 to 2016, virus surveys were conducted in several potato-growing governorates of Egypt, and PVY-positive samples were found to represent at least five distinct recombinant PVY strains, including PVYNTN and PVYN-Wi. Whole genome sequences were determined for four isolates representing strains PVY-SYR-III (Egypt7), PVY-261-4 (Egypt11), PVYNTNa (Egypt35), and a novel recombinant named Egypt24 that combined molecular properties of strains PVY-261-4 and PVY-Wilga156var. At least three recombinants found in Egypt in potato were previously found associated with potato tuber necrotic ringspot disease (PTNRD). The identification of multiple recombinant types of PVY in potato in Egypt, including the novel recombinant Egypt24, suggests a wide presence of PTNRD-inducing virus strains in the country.

scholarly journals Potato Tuber Necrotic Ringspot Disease Occurring in Japan: Its Association with Potato virus Y Necrotic Strain

Plant Disease ◽  
2000 ◽  
Vol 84 (10) ◽  
pp. 1109-1115 ◽  
Author(s):  
Kazusato Ohshima ◽  
Kazuya Sako ◽  
Chikako Hiraishi ◽  
Akio Nakagawa ◽  
Kazutoshi Matsuo ◽  
...  
Keyword(s):  
Amino Acids ◽  
Coat Protein ◽  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Nucleotide Sequences ◽  
Potato Tubers ◽  
Necrotic Spots ◽  
Nagasaki Prefecture ◽  
Necrotic Symptoms

Unfamiliar necrotic symptoms on or within potato tubers of cultivars Nishiyutaka and Dejima were observed in Nagasaki prefecture, Japan, in 1992. Symptoms were typically on the surface of the tuber, which either protruded at first and then became sunken, or showed necrotic spots, with necrosis within the tubers. Symptoms sometimes appeared at harvesting but more often appeared after storage for several months. Investigations revealed that the causal agents of the disease were isolates of Potato virus Y necrotic strain (PVYNTN) and the disease was potato tuber necrotic ringspot disease (PTNRD), previously reported in Europe and Lebanon. Five potato cultivars were inoculated with an isolate from necrotic tubers. The highest percentage of progeny tubers showing PTNRD was found in cv. Nishiyutaka (23.3%). In contrast, cvs. Shima-bara, Mayqueen, and Danshaku showed a low percentage of PTNRD. Additional potato tubers with PTNRD were also observed after storage of the tubers. To investigate the relatedness between isolates of PVYNTN and of necrotic strain PVYN, previously isolated in Japan, Nishiyu-taka was inoculated with an isolate of PVYN, which also induced PTNRD. Nucleotide sequences of coat protein (CP) genes of six PVYNTN isolates were determined. The CPs were 267 amino acids in length, with a substitution of one or no amino acid among each of the six isolates. The phylogenetic relationship based on nucleotide sequences of CP genes showed that these six PVYNTN isolates clustered together with PVYN isolates. This is the first report of PTNRD caused by PVYNTN isolates in Asia.

scholarly journals Molecular characterization of potato virus y inducing potato tuber necrotic ringspot disease in Serbia

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 487-496 ◽  
Author(s):  
Danijela Ristic ◽  
Ivan Vucurovic ◽  
Slobodan Kuzmanovic ◽  
Drago Milosevic ◽  
Katarina Gasic ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Potato Tuber ◽  
Molecular Characterization ◽  
Potato Virus ◽  
Complete Genome ◽  
Potato Virus Y ◽  
Bootstrap Support ◽  
Limiting Factor ◽  
Potato Seed ◽  
Tuber Necrosis

The Potato virus Y (PVY) is the most important limiting factor for potato seed production in Serbia. Currently, PVY is a major concern for the potato seed growers. Initially, serological (ELISA) tests were carried out on 100 potato seed tubers from each of the seven potato cv. during 2013. The infection rates with the PVYN was between 5 and 36%. A complete genome sequencing of the most common Serbian isolate of PVY (3D), followed by molecular characterization and phylogenetic analysis has been performed to show what group it belongs to. Our isolate?s complete genome sequence (KJ946936) showed that the Serbian PVY isolate (3D) is 99.7% identical at nt level, with other tuber necrosis strain group (PVYNTN) from Europe. Phylogenetic analysis revealed three consistent lineages of isolates, showing that our isolate was clustered with the isolates from Europe and North America in the PVYN lineage which induces potato tuber necrotic ringspot disease (PTNRD). The Serbian isolate of PVYNTN together with the isolates from Europe was clustered in the branch of European sublineage, with a high bootstrap support and no genetic diversity. This is the first study in Serbia demonstrating phylogenetic distinction between our isolate and other isolates of PVY.

Characteristics of a resistance-breaking isolate of potato virus Y causing potato tuber necrotic ringspot disease

1994 ◽  
Vol 100 (5) ◽  
pp. 347-356 ◽  
Author(s):  
J. F. J. M. van den Heuvel ◽  
R. A. A. van der Vlugt ◽  
M. Verbeek ◽  
P. T. de Haan ◽  
H. Huttinga
Keyword(s):  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Resistance Breaking

scholarly journals Effects of the Age-Related Resistance to Potato virus Y in Potato on the Systemic Spread of the Virus, Incidence of the Potato Tuber Necrotic Ringspot Disease, Tuber Yield, and Translocation Rates Into Progeny Tubers

Plant Disease ◽  
2020 ◽  
Vol 104 (1) ◽  
pp. 269-275 ◽  
Author(s):  
Mohamad Chikh-Ali ◽  
Lisa T. Tran ◽  
William J. Price ◽  
Alexander V. Karasev
Keyword(s):  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Potato Cultivar ◽  
Yield Reduction ◽  
Systemic Movement ◽  
Age Related ◽  
Main Challenge ◽  
Systemic Spread

The recombinant strain of potato virus Y (PVY), PVYNTN, is the main cause of the potato tuber necrotic ringspot disease (PTNRD) in susceptible potato cultivars, which reduces the quality of potato tubers, in addition to the yield loss. Control of PVY has been the main challenge in most potato-producing areas. Here, the effects of the age-related resistance (ARR) were investigated in transplants of a potato cultivar Yukon Gold to the infection with PVYNTN strain in greenhouse experiments. Within the first 3 weeks after transplanting into soil (week 1 [W1] to W3), Yukon Gold plants developed ARR that impaired the systemic movement of PVYNTN into upper noninoculated leaves and concomitant translocation into progeny tubers starting from W4 after transplanting. The yield and quality of tubers from PVY-infected plants with the established ARR (W5 to W8) were comparable with the healthy controls, suggesting that late PVY infection would not significantly affect commercial potato production. Plants inoculated early (W1 to W2), before the establishment of the ARR, exhibited a 100% primary systemic infection with PVYNTN and produced fewer tubers of smaller sizes, exhibiting PTNRD; this resulted ≤70% yield reduction compared with plants inoculated later in the season (W5 to W8). This ARR greatly restricted the systemic movement of PVYNTN in the foliage and resulted in very limited translocation rates of the virus into tested progeny tubers: 7.8 and 4.1% in 2017 and 2018, respectively, of all plants inoculated later in the season (W5 to W8). This study suggests that PVYNTN management programs in Yukon Gold seed potato should focus more on the early stages of the potato development before the onset of the ARR.

scholarly journals Factors influencing detection of Potato Leafroll Virus and Potato Virus Y in potato tuber extracts

2009 ◽  
Vol 59 (1) ◽  
pp. 65-74 ◽  
Author(s):  
Krzysztof Treder ◽  
Włodzimierz Przewodowski ◽  
Agnieszka Barnyk
Keyword(s):  
Potato Tuber ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Enzymatic Reaction ◽  
Potato Leafroll Virus ◽  
Dormancy Breaking ◽  
Signal Ratio ◽  
Factors Influencing ◽  
Leafroll Virus ◽  
Das Elisa

Factors influencing detection of Potato Leafroll Virus and Potato Virus Y in potato tuber extracts Detection of Potato leafroll virus (PLRV) and Potato virus Y (PVY) directly in potato tubers has been influenced by several factors. The most important were: the place of tuber sampling, preincubation of tuber sap before loading into wells of microplate and duration of tubers storage after collecting from field. The concentration of both viruses was highest in the heel part of tubers, whenever tested. Preincubation of tuber sap for several hours improved true/false signal ratio for dormant tubers and enabled reliable detection of both viruses. However after natural dormancy breaking it was necessary to change Cocktail-ELISA procedure to obtain reliable results, consistent with DAS-ELISA on leaves. The sap was not preincubated but loaded into wells directly after sample collecting and immuno-enzymatic reaction was developed overnight in refrigerator.

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