Oxygen and carbon dioxide treatment to break potato tuber dormancy for reliable detection of potato virus Y (PVY) by enzyme-linked immunosorbent assay (ELISA)

Polerovirus: potato leaf roll virus (PLRV), Potyvirus: potato virus Y (PVY) and Potexvirus: potato virus X (PVX) is more destructive and well distributed throughout the Pakistan. Incidence has been reported to be as high as 90%, 25%, and ≥ 15%, respectively in the potato growing regions. To find out the source of resistance, twenty-nine virus free potato varieties were grown under field conditions with good agricultural practices. The disease severity of PLRV, PVY and PVX was recorded to determine the level of resistance of the potato varieties according to the disease rating scale. Infectivity and biological assay of all twenty-nine varieties were done in green house on potato, Datura stramonium, Nicotiana glutinosa and Physalis floridana. Non-inoculated plants were served as control. Leaf samples from potato varieties were collected for serological detection of PLRV, PVY and PVX by Double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). Out of twenty nine varieties, none of the variety was resistant to PLRV although three varieties; Mirrato, 394021-120 and Orla were moderately resistant. Only FD 48-4 and TPS 9813 showed resistance to PVX and PVY. While FD 3-10, FD 9616 and FD 37-13 were moderately to PVX and PVY. Rest of the varieties was found susceptible to all three viruses.

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The use of enzyme — linked immunosorbent assay (ELISA) for quantitative detection of potato virus Y in potato and other test plants

Microbiological Research ◽

10.1016/s0944-5013(97)80045-6 ◽

1997 ◽

Vol 152 (3) ◽

pp. 307-313 ◽

Cited By ~ 1

Author(s):

Mahasen Hassan Ismail

Keyword(s):

Immunosorbent Assay ◽

Potato Virus ◽

Potato Virus Y ◽

Quantitative Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Test Plants

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Enzyme-linked immunosorbent assay (ELISA) for the detection of potato leafroll virus and potato virus Y in potato tubers after artificial break of dormancy

Potato Research ◽

10.1007/bf02360674 ◽

1980 ◽

Vol 23 (3) ◽

pp. 353-359 ◽

Cited By ~ 48

Author(s):

P. Gugerli ◽

W. Gehriger

Keyword(s):

Immunosorbent Assay ◽

Potato Virus ◽

Potato Virus Y ◽

Potato Leafroll Virus ◽

Enzyme Linked Immunosorbent Assay ◽

Potato Tubers ◽

Leafroll Virus

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Resistance to homologous and heterologous strains of potato virus Y in transgenic tobacco carrying the PVYN coat protein gene

Canadian Journal of Plant Science ◽

10.4141/p96-005 ◽

1997 ◽

Vol 77 (1) ◽

pp. 167-171 ◽

Cited By ~ 4

Author(s):

J. G. McDonald ◽

J. E. Brandle ◽

S. Gleddie ◽

J. A. Hermans ◽

I. R. Kermali

Keyword(s):

Nicotiana Tabacum ◽

Coat Protein ◽

Immunosorbent Assay ◽

Potato Virus ◽

Coat Protein Gene ◽

Potato Virus Y ◽

Protein Gene ◽

Enzyme Linked Immunosorbent Assay ◽

Strain Resistance ◽

Transgenic Nicotiana Tabacum

Pure breeding lines of transgenic Nicotiana tabacum L. 'Delgold' were generated which expressed transcripts of the coat protein gene of the tobacco veinal necrosis stain of potato virus Y (PVYN) and were highly resistant to the virus. The form of resistance appeared to be RNA-mediated as no coat protein could be detected by enzyme linked immunosorbent assay. Resistant lines had two or three copies of the transgene but segregation analysis of the R1 and R2 progeny indicated that one copy in each line was functional for virus resistance. Simultaneous screening of lines for resistance against both PVYN and the heterologous PVYO (common) strain allowed for optimal selection of heterologous strain resistance. Resistance against the PVY-mn and PVY-nn strains and an ungrouped isolate, PVY-136, was less than against the PVYO isolate. The degree of heterologous strain resistance did not appear to directly correlate with the degree of sequence homology previously reported. The lines had no resistance against the related potato virus A and pepper mottle virus. Key words: Resistance to potato virus Y, transgenic, Nicotiana tabacum, enzyme linked immunosorbent assay, monoclonal antibodies

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Monitoring Current-Season Spread of Potato virus Y in Potato Fields Using ELISA and Real-Time RT-PCR

Plant Disease ◽

10.1094/pdis-03-12-0283-re ◽

2013 ◽

Vol 97 (5) ◽

pp. 641-644 ◽

Cited By ~ 17

Author(s):

Manphool S. Fageria ◽

Mathuresh Singh ◽

Upeksha Nanayakkara ◽

Yvan Pelletier ◽

Xianzhou Nie ◽

...

Keyword(s):

Real Time ◽

Potato Virus ◽

Potato Virus Y ◽

Enzyme Linked Immunosorbent Assay ◽

Rt Pcr ◽

Pcr Assay ◽

Current Season ◽

Seed Market ◽

Polymerase Chain ◽

Time Of Harvest

The current-season spread of Potato virus Y (PVY) was investigated in New Brunswick, Canada, in 11 potato fields planted with six different cultivars in 2009 and 2010. In all, 100 plants selected from each field were monitored for current-season PVY infections using enzyme-linked immunosorbent assay (ELISA) and real-time reverse-transcription polymerase chain reaction (RT-PCR) assay. Average PVY incidence in fields increased from 0.6% in 2009 and 2% in 2010 in the leaves to 20.3% in 2009 and 21.9% in 2010 in the tubers at the time of harvest. In individual fields, PVY incidence in tubers reached as high as 37% in 2009 and 39% in 2010 at the time of harvest. Real-time RT-PCR assay detected more samples with PVY from leaves than did ELISA. A higher number of positive samples was also detected with real-time RT-PCR from growing tubers compared with the leaves collected from the same plant at the same sampling time. PVY incidence determined from the growing tubers showed a significant positive correlation with the PVY incidence of tubers after harvest. Preharvest testing provides another option to growers to either top-kill the crop immediately to secure the seed market when the PVY incidence is low or leave the tubers to develop further for table or processing purposes when incidence of PVY is high.

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Epidemic of Potato virus Y and Cucumber mosaic virus in Henan Province Tobacco

Plant Disease ◽

10.1094/pdis.2001.85.4.447c ◽

2001 ◽

Vol 85 (4) ◽

pp. 447-447 ◽

Cited By ~ 1

Author(s):

X. D. Li ◽

Y. Q. Li ◽

H. G. Wang

Keyword(s):

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Potato Virus ◽

Potato Virus Y ◽

Early Stage ◽

Potato Virus X ◽

Henan Province ◽

Enzyme Linked Immunosorbent Assay ◽

Resistant Varieties ◽

Vein Necrosis

Flue-cured tobacco is an important crop in Henan Province, China. During the 2000 growing season, many tobacco plants showed various degrees of mottling, mosaic, vein clearing, or vein necrosis in most of the counties. Some plants even died at an early stage of growth. A survey was conducted in May-June in several tobacco-growing counties, and the incidence of symptomatic plants in individual fields ranged from 10 to 85%. The most widely planted tobacco varieties, NC89, K326, and K346, were highly susceptible. Symptomatic plants were collected from Jiaxian and Xiangcheng counties and samples were tested by enzyme-linked immunosorbent assay for Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Potato virus Y (PVY), and Potato virus X (PVX). Of 65 samples tested, 21 were positive for only PVY, 16 positive for only CMV, one each was positive for only TMV or PVX. Nineteen samples were doubly infected with various combinations of these viruses and six were infected with combinations of three viruses. The causal agent(s) in the remaining sample could not be determined. In total, CMV was detected in 40 samples, PVY in 38, PVX in 10, and TMV in 7 samples. TMV and CMV used to be the most important viruses and PVY occurred only rarely. But PVY has become prevalent in Henan and in neighboring Shandong province (2). CMV and TMV were reported to be the most prevalent viruses in Shanxi (1) and Fujian Provinces (3). Because resistant varieties are not available, and mixed infections are more common, the results presented here explain why huge damage is occurring in tobacco crops in recent years. Some varieties are partially resistant to TMV and CMV but the varieties commonly grown are highly susceptible to PVY. Therefore, breeding for resistance to viruses, especially to PVY, is urgent to control the occurrence of tobacco viral diseases. References: (1) J. L. Cheng et al. Acta Tabacaria Sin. 4:43, 1998. (2) J. B. Wang et al. Chinese Tobacco Sci. 1:26, 1998. (3) L. H. Xie et al. Acta Tabacaria Sin. 2:25, 1994.

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