Genetic diversity, presence of the syrB gene, host preference and virulence of Pseudomonas syringae pv. syringae strains from woody and herbaceous host plants

<div><div><div><p>Pseudomonas syringae is an important pathogen of many agriculturally valuable crops. Among the various pathovars described P. syringae pv. syringae (Pss) has a particularly wide host range, infecting primarily woody and herbaceous host plants. The ability of Pss to cause bacterial apical necrosis of mango trees is dependent on the production of the antimetabolite toxin mangotoxin. The production of this toxin was shown to be regulated by a self-produced signaling molecule. In this study, we determined the structure of the Pss signal molecule belonging to the recently described family of diazeniumdiolate communication molecules. Employing a targeted mass spectrometry-based approach, we provide experimental evidence that the major signal produced by Pss is the volatile compound leudiazen, which controls mangotoxin production and virulence in a detached tomato leaflet infection model. Experimental results demonstrate that KMnO4 solution inactivates leudiazen and that treatment of infected leaves with KMnO<sub>4</sub> abolishes necrosis. This strategy represents the first example of chemically degrading a signaling molecule to interfere with bacterial communication. The application of KMnO4 solution, which is regulatorily approved in organic farming, may constitute an environmentally friendly strategy to control Pss infections.</p></div></div></div>

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A Volatile Signal Controls Virulence in the Plant Pathogen Pseudomonas syringae pv. syringae and a Strategy for Infection Control in Organic Farming

10.1101/2020.09.18.279364 ◽

2020 ◽

Author(s):

Simon Sieber ◽

Anugraha Mathew ◽

Christian Jenul ◽

Tobias Kohler ◽

Max Bär ◽

...

Keyword(s):

Organic Farming ◽

Pseudomonas Syringae ◽

Volatile Compound ◽

Host Plants ◽

Infection Model ◽

Signal Molecule ◽

Signaling Molecule ◽

Bacterial Communication ◽

Herbaceous Host ◽

Apical Necrosis

AbstractPseudomonas syringae is an important pathogen of many agriculturally valuable crops. Among the various pathovars described P. syringae pv. syringae (Pss) has a particularly wide host range, infecting primarily woody and herbaceous host plants. The ability of Pss to cause bacterial apical necrosis of mango trees is dependent on the production of the antimetabolite toxin mangotoxin. The production of this toxin was shown to be regulated by a self-produced signaling molecule. In this study, we determined the structure of the Pss signal molecule belonging to the recently described family of diazeniumdiolate communication molecules. Employing a targeted mass spectrometry-based approach, we provide experimental evidence that the major signal produced by Pss is the volatile compound leudiazen, which controls mangotoxin production and virulence in a detached tomato leaflet infection model. Experimental results demonstrate that KMnO4 solution inactivates leudiazen and that treatment of infected leaves with KMnO4 abolishes necrosis. This strategy represents the first example of chemically degrading a signaling molecule to interfere with bacterial communication. The application of KMnO4 solution, which is regulatorily approved in organic farming, may constitute an environmentally friendly strategy to control Pss infections.

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A Volatile Signal Controls Virulence in the Plant Pathogen Pseudomonas syringae pv. syringae and a Strategy for Infection Control in Organic Farming

10.26434/chemrxiv.12906551.v1 ◽

2020 ◽

Author(s):

Simon Sieber ◽

Anugraha Mathew ◽

Christian Jenul ◽

Tobias Kohler ◽

Max Bär ◽

...

Keyword(s):

Organic Farming ◽

Pseudomonas Syringae ◽

Volatile Compound ◽

Host Plants ◽

Infection Model ◽

Signal Molecule ◽

Signaling Molecule ◽

Bacterial Communication ◽

Herbaceous Host ◽

Apical Necrosis

<div><div><div><p>Pseudomonas syringae is an important pathogen of many agriculturally valuable crops. Among the various pathovars described P. syringae pv. syringae (Pss) has a particularly wide host range, infecting primarily woody and herbaceous host plants. The ability of Pss to cause bacterial apical necrosis of mango trees is dependent on the production of the antimetabolite toxin mangotoxin. The production of this toxin was shown to be regulated by a self-produced signaling molecule. In this study, we determined the structure of the Pss signal molecule belonging to the recently described family of diazeniumdiolate communication molecules. Employing a targeted mass spectrometry-based approach, we provide experimental evidence that the major signal produced by Pss is the volatile compound leudiazen, which controls mangotoxin production and virulence in a detached tomato leaflet infection model. Experimental results demonstrate that KMnO4 solution inactivates leudiazen and that treatment of infected leaves with KMnO<sub>4</sub> abolishes necrosis. This strategy represents the first example of chemically degrading a signaling molecule to interfere with bacterial communication. The application of KMnO4 solution, which is regulatorily approved in organic farming, may constitute an environmentally friendly strategy to control Pss infections.</p></div></div></div>

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Host preference of Pentalonia nigronervosa Coquerel and P. caladii van der Goot (Hemiptera: Aphididae) on various host plants

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/694/1/012050 ◽

2021 ◽

Vol 694 (1) ◽

pp. 012050

Author(s):

S Rahmah ◽

N Maryana ◽

P Hidayat

Keyword(s):

Host Plants ◽

Host Preference ◽

Pentalonia Nigronervosa

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The type III effector repertoire of Pseudomonas syringae pv. syringae B728a and its role in survival and disease on host and non-host plants

Molecular Microbiology ◽

10.1111/j.1365-2958.2006.05350.x ◽

2006 ◽

Vol 62 (1) ◽

pp. 26-44 ◽

Cited By ~ 128

Author(s):

Boris A. Vinatzer ◽

Gail M. Teitzel ◽

Min-Woo Lee ◽

Joanna Jelenska ◽

Sara Hotton ◽

...

Keyword(s):

Pseudomonas Syringae ◽

Host Plants ◽

Type Iii Effector ◽

Type Iii

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The lifecycle and epidemiology of Bactericera cockerelli on three traditional Maori food sources

New Zealand Plant Protection ◽

10.30843/nzpp.2010.63.6584 ◽

2010 ◽

Vol 63 ◽

pp. 275-275

Author(s):

A.J. Puketapu

Keyword(s):

New Zealand ◽

Ipomoea Batatas ◽

Host Plants ◽

Host Preference ◽

Life Stage ◽

Food Sources ◽

Bactericera Cockerelli ◽

Plant Remains ◽

Introduced Pest ◽

Solanum Aviculare

The tomato/potato psyllid Bactericera cockerelli (Sulc) (Hemiptera Triozidae) is an introduced pest of solanaceous crops in New Zealand A range of established plants play host to Bactericera cockerelli including three traditional Maori food sources taewa or Maori potatoes (Solanum tuberosum ssp andigena) kumara (Ipomoea batatas) and poroporo (Solanum aviculare) Taewa and kumara are highly susceptible to summer B cockerelli infestation whilst poroporo an evergreen plant remains susceptible yearround and provides overwintering refuge Extensive monitoring of each host plant was carried out to determine the significance of each host in the lifecycle of B cockerelli in New Zealand Poroporo was monitored from late autumn for 6 months to determine if the plant served as a significant overwintering host for the pest after harvesting summer crops Taewa and kumara plants were monitored throughout the summer growing season on a weekly basis increasing to twice a week as populations proliferated Host plants were monitored both in the natural environment and under laboratory conditions Data collected contributed to tracking population development of B cockerelli on each host including the length of each life stage (ie egg nymph adult) Comparisons between the three hosts revealed host preference host suitability and the significance of each host in the lifecycle progression of B cockerelli

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Draft Genome Resources Sequences of Six Pseudomonas syringae pv. actinidiae Strains Isolated from Actinidia chinensis var. deliciosa Leaves in Portugal

Phytopathology ◽

10.1094/phyto-05-20-0184-a ◽

2020 ◽

pp. PHYTO-05-20-018

Author(s):

Aitana Ares ◽

Marta Tacão ◽

Daniela Figueira ◽

Eva Garcia ◽

Joana Costa

Keyword(s):

Genetic Diversity ◽

Pseudomonas Syringae ◽

Draft Genome ◽

Clonal Expansion ◽

Average Nucleotide Identity ◽

Actinidia Chinensis ◽

Valuable Resource ◽

Genetic Population ◽

Evolutionary Studies ◽

Reference Strains

Pseudomonas syringae pv. actinidiae is a quarantine bacterium affecting all the Portuguese main areas of kiwifruit production. We report the draft genome of six P. syringae pv. actinidiae strains isolated from symptomatic leaves of Actinidia chinensis var. deliciosa in a study that determined the genetic population structure of the endophytic and epiphytic populations in two consecutive seasons. Average nucleotide identity values were above 99% similarity with reference strains from P. syringae pv. actinidiae biovar 3. The genomic differences found between these strains confirm the genetic diversity described for P. syringae pv. actinidiae population in Portugal. Furthermore, data provide evidence that the initial clonal expansion of P. syringae pv. actinidiae in Europe was followed by a genomic diversification constituting a valuable resource for epidemiological and evolutionary studies, namely when adopting strategies for epidemics management.

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