Immunohistochemical expression of neural tissue markers (neuron-specific enolase, glial fibrillary acidic protein, S100 protein) in ameloblastic fibrodentinoma: A comparative study with ameloblastic fibroma

2000 ◽  
Vol 50 (8) ◽  
pp. 610-615 ◽  
Author(s):  
Yasunori Takeda ◽  
Hirotaka Sato ◽  
Masanobu Satoh ◽  
Shin-ichi Nakamura ◽  
Hirotsugu Yamamoto
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Comparative Study ◽  
S100 Protein ◽  
Neuron Specific Enolase ◽  
Neural Tissue ◽  
Acidic Protein ◽  
Immunohistochemical Expression ◽  
Ameloblastic Fibroma

scholarly journals Glial Fibrillary Acidic Protein (GFAP) in Oligodendrogliomas: A Reflection of Transient GFAP Expression by Immature Oligodendroglia

1986 ◽  
Vol 13 (4) ◽  
pp. 307-311 ◽  
Author(s):  
V. Jagadha ◽  
W.C. Halliday ◽  
L.E. Becker
Keyword(s):  
White Matter ◽  
Glial Fibrillary Acidic Protein ◽  
Tumor Cells ◽  
Intermediate Filaments ◽  
Intermediate Filament ◽  
Immunohistochemical Staining ◽  
Neuron Specific Enolase ◽  
Acidic Protein ◽  
Gfap Expression ◽  
S 100

ABSTRACT:Fourteen pure oligodendrogliomas were studied by light- and electronmicroscopy and immunohistochemistry to examine glial fibrillary acidic protein (GFAP) positivity in the tumors. To compare the immunohistochemical staining patterns of neoplastic oligodendroglia and immature oligodendroglia, myelination glia in the white matter of eight normal brains from children under 6 months of age were studied. The tumors possessed light microscopic and ultrastructural features characteristic of oligodendrogliomas. Microtubules were found in the cytoplasm of nine tumors on electronmicroscopy. In one, intermediate filaments and microtubules were observed in occasional tumor cells with polygonal crystalline structures in the cytoplasm. Using the peroxidase-antiperoxidase technique, all specimens were stained for GFAP, vimentin, S-100 and neuron-specific enolase (NSE). In nine tumors, variable numbers of cells with an oligodendroglial morphology reacted positively for GFAP. All tumors were positive for S-100 and negative for vimentin and NSE. The myelination glia in the eight normal brains stained positively for GFAP but not for vimentin. Vimentin is expressed by developing, reactive and neoplastic astrocytes. Thus, GFAP positivity combined with vimentin negativity in both neoplastic and immature oligodendroglia suggests that GFAP positivity in oligodendrogliomas may reflect the transient expression of this intermediate filament by immature oligodendroglia.

Mechanism of S100 protein-dependent inhibition of glial fibrillary acidic protein (GFAP) polymerization

1994 ◽  
Vol 1223 (3) ◽  
pp. 354-360 ◽  
Author(s):  
Roberta Bianchi ◽  
Marco Verzini ◽  
Marisa Garbuglia ◽  
Ileana Giambanco ◽  
Rosario Donato
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
S100 Protein ◽  
Acidic Protein ◽  
Dependent Inhibition

Identification of Central Nervous System Tissue in Retail Meat Products†

2000 ◽  
Vol 63 (2) ◽  
pp. 258-263 ◽  
Author(s):  
ERNST H. LÜCKER ◽  
ERICH EIGENBRODT ◽  
SABINE WENISCH ◽  
RUDI LEISER ◽  
MICHAEL BÜ]LTE
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Glial Fibrillary Acidic Protein ◽  
Meat Products ◽  
Neuron Specific Enolase ◽  
Cholesterol Content ◽  
Acidic Protein ◽  
Central Nervous System Tissue ◽  
Heat Treated ◽  
Retail Meat

A procedure to detect tissues from the central nervous system that involved quantification of cholesterol and immuno-chemical detection of neuron-specific enolase and glial fibrillary acidic protein was used to analyze 402 samples of heat-treated meat products from various food outlets in Germany. The cholesterol content of 16 samples (4.0%) indicated the possible presence of central nervous system tissue because the levels exceeded the normal maximum cholesterol content of cooked sausages. In 7 of these 16 heat-treated meat products, immunoblotting of both neuron-specific enolase and glial fibrillary acidic protein confirmed the presence of CNS tissue. Repeated sampling by veterinary officials and analysis by both cholesterol quantification and immunoblotting confirmed these findings. Whereas all of the control samples (with and without added central nervous system tissue) were correctly classified by both cholesterol quantification and immunoblotting, negative results of immunoblotting must be carefully interpreted in the case of intensively heat-treated meat products. Thus, studies have yet to establish an increase in sensitivity of immunoblotting of neuron-specific enolase and glial fibrillary acidic protein. However, the detection of illegal use of central nervous system tissue in heat-treated retail meat products demonstrates the need for suitable analytical methods to control transmissible encephalopathies and to enforce labeling laws.

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