Immunoglobulins and Complement in Migraine

In 54 patients with migraine and 70 persons comprising the control group, the total complement level (CH50) was evaluated together with its C3 and C4 fraction level and the level of IgG, IgA and IgM immunoglobulins. It was found that the average C3 fraction level was significantly decreased, while the C4 fraction level and total complement activity remained in the normal range. The immunoglobulin level did not show any statistically significant alterations except for the IgA level, which was lowered in migraine patients. On this basis, i.e. lowering of the C3 fraction level with normal C4 fraction level and total complement activity and lack of elevation of IgA, it is felt that the alternative pathway of complement system may be activated in migraine.

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Local Activation of the Alternative Pathway of Complement System in Mycotic Keratitis Patient Tear

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2020.00205 ◽

2020 ◽

Vol 10 ◽

Author(s):

Mohammed Razeeth Shait Mohammed ◽

Sandhya Krishnan ◽

Rabbind Singh Amrathlal ◽

Jeya Maheshwari Jayapal ◽

Venkatesh Prajna Namperumalsamy ◽

...

Keyword(s):

Complement System ◽

Alternative Pathway ◽

Mycotic Keratitis ◽

Local Activation ◽

Alternative Pathway Of Complement

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Mathematical Modelling of Alternative Pathway of Complement System

Bulletin of Mathematical Biology ◽

10.1007/s11538-020-00708-z ◽

2020 ◽

Vol 82 (2) ◽

Cited By ~ 1

Author(s):

Suruchi Bakshi ◽

Fraser Cunningham ◽

Eva-Maria Nichols ◽

Marta Biedzka-Sarek ◽

Jessica Neisen ◽

...

Keyword(s):

Mathematical Modelling ◽

Complement System ◽

Alternative Pathway ◽

Alternative Pathway Of Complement

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Correction to: Mathematical Modelling of Alternative Pathway of Complement System

Bulletin of Mathematical Biology ◽

10.1007/s11538-021-00981-6 ◽

2021 ◽

Vol 84 (1) ◽

Author(s):

Suruchi Bakshi ◽

Fraser Cunningham ◽

Eva-Maria Nichols ◽

Marta Biedzka-Sarek ◽

Jessica Neisen ◽

...

Keyword(s):

Mathematical Modelling ◽

Complement System ◽

Alternative Pathway ◽

Alternative Pathway Of Complement

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Determination of alternative pathway of complement activity in mouse serum using rabbit erythrocytes

Journal of Immunological Methods ◽

10.1016/0022-1759(80)90091-5 ◽

1980 ◽

Vol 36 (1) ◽

pp. 29-39 ◽

Cited By ~ 34

Author(s):

Hans Van Dijk ◽

Pieternel M. Rademaker ◽

Jan M.N. Willers

Keyword(s):

Alternative Pathway ◽

Mouse Serum ◽

Complement Activity ◽

Alternative Pathway Of Complement

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Homogeneous, liposome-based assay for total complement activity in serum.

Clinical Chemistry ◽

10.1093/clinchem/32.2.275 ◽

1986 ◽

Vol 32 (2) ◽

pp. 275-278 ◽

Cited By ~ 11

Author(s):

D W Bowden ◽

M Rising ◽

G Akots ◽

A Myles ◽

R J Broeze

Keyword(s):

Normal Population ◽

Test Results ◽

Normal Range ◽

Serum Samples ◽

Complement Components ◽

Complement Activity ◽

Standard Curve ◽

Total Complement ◽

Speed Accuracy ◽

Range Test

Abstract This is a rapid, homogeneous, liposome-based assay for total complement activity in human serum. Liposome-encapsulated enzyme is unmasked by the action of complement on liposomes carrying surface-bound immune complexes. The amount of unmasked enzyme, proportional to the concentration of added complement, is quantified by measuring the absorbance of enzymically produced product at 410 nm. Complement activity in serum samples is extrapolated from a standard curve generated from dilutions of a guinea pig serum containing a known activity of complement. Interassay CVs were less than 7.0% and intra-assay CVs less than 2.8% for serum pools with complement activities spanning the normal range. Test results correlate as well with those of the hemolytic complement test (r = 0.80) as the latter correlates with itself (r = 0.82), and also correlate reasonably with measurements of complement components C3 (r = 0.62) and C4 (r = 0.74). Values for a normal population are reported. Advantages of this test include stability of reagents, speed, accuracy, simplicity, and avoidance of radioisotopes.

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Complement system component dysregulation is a distinctive feature of COVID-19 disease: a prospective and comparative analysis of patients admitted to the emergency department for suspected COVID-19 disease

Journal of Thrombosis and Thrombolysis ◽

10.1007/s11239-021-02617-x ◽

2021 ◽

Author(s):

Nadine Gauchel ◽

Marina Rieder ◽

Krystin Krauel ◽

Isabella Goller ◽

Maren Jeserich ◽

...

Keyword(s):

Emergency Department ◽

Complement System ◽

Prospective Trial ◽

System Component ◽

Control Group ◽

Classical Pathway ◽

Prospective Data ◽

Total Complement ◽

Von Willebrand ◽

Willebrand Factor

AbstractThe complement system (CS) plays a pivotal role in Coronavirus disease 2019 (COVID-19) pathophysiology. The objective of this study was to provide a comparative, prospective data analysis of CS components in an all-comers cohort and COVID-19 patients. Patients with suspected COVID-19 infection admitted to the Emergency department were grouped for definite diagnosis of COVID-19 and no COVID-19 accordingly. Clinical presentation, routine laboratory and von Willebrand factor (vWF) antigen as well as CS components 3, 4 and activated 5 (C5a) were assessed. Also, total complement activity via the classical pathway (CH50) was determined. Levels of calprotectin in serum were measured using an automated quantitative lateral flow assay. We included 80 patients in this prospective trial. Of those 19 (23.7%) were tested positive for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Patients with COVID-19 had higher levels of CS components 5a and 4 (54.79 [24.14–88.79] ng/ml vs. 35 [23.15–46.1] ng/ml; p = 0.0433 and 0.3772 [± 0.1056] g/L vs. 0.286 [0.2375–0.3748] g/L; p = 0.0168). COVID-19 patients had significantly higher levels of vWF antigen when compared to the control group (288.3 [± 80.26] % vs. 212 [151–320] %; p = 0.0469). There was a significant correlation between CS C3 and 5a with vWF antigen (rs = 0.5957 [p = 0.0131] and rs = 0.5015 [p = 0.042]) in COVID-19 patients. There was no difference in calprotectin plasma levels (4.786 [± 2.397] µg/ml vs. 4.233 [± 2.142] µg/ml; p = 0.4175) between both groups. This prospective data from a single centre all-comers cohort accentuates altered levels of CS components as a distinct feature of COVID-19 disease. Deregulation of CS component 3 and C5a are associated with increased vWF antigen possibly linking vascular damage to alternative CS activation in COVID-19.

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NEUTROPHILS FROM ANCA-ASSOCIATED VASCULITIS PATIENTS SHOW AN INCREASED CAPACITY TO ACTIVATE THE COMPLEMENT SYSTEM VIA THE ALTERNATIVE PATHWAY

10.26226/morressier.56e174ded462b8028d88b00b ◽

2016 ◽

Author(s):

Sophie Ohlsson

Keyword(s):

Complement System ◽

Alternative Pathway ◽

Anca Associated Vasculitis ◽

The Complement System

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POSSIBILITY TO REDUCE THROMBOCYTOPENIA AFTER CHEMORADIOTHERAPY IN ONCOLOGICAL PATIENTS

Problems in oncology ◽

10.37469/0507-3758-2017-63-3-466-469 ◽

2017 ◽

Vol 63 (3) ◽

pp. 466-469

Author(s):

Luiza Korytova ◽

Aleksey Meshechkin ◽

Oleg Korytov ◽

V. Krasnikova

Keyword(s):

Combined Treatment ◽

Treated Group ◽

Median Number ◽

Blood Analysis ◽

Control Group ◽

Level Control ◽

Normal Range ◽

Oncological Patients ◽

Level Increase ◽

And Control

Objective was to establish efficiency of sodium nucleospermat in correcting thrombocytopenia after chemoradiotherapy in oncological patients. Methods and materials. The study included data on 32 patients that had undergone combined treatment from January till May 2016. After detecting thrombocytopenia patients were randomized into two groups (16 patients in each): treated group, where patients received sodium nucleospermat, and control group, where sodium nucleospermat was not used. Thrombocyte level control was done on 5th, 10th and 15th day after treatment was over. Results and discussion. All 16 patients showed positive dynamics in increasing thrombocyte level after Sodium nucleospermat injection course was finished. This was proven by first (5th day) blood analysis. On average thrombocyte level after sodium nucleospermat treatment has risen to normal, at 161х109/1. Only 3 patients from this group had to pause radiotherapy for 5 days. Control group patients, which did not receive sodium nucleospermat, showed evidence of thrombocyte level recovery by 10th day only. On average thrombocyte level increase was insignificant, and median number was 111*109/l. Low thrombocyte level was main reason to pause radiotherapy for 11 (69%) patients in control group. Conclusion. Sodium nucleospermat allowed raising thrombocyte level to the lower normal range, which surpassed by 40%-50% in control group patients. Use of sodium nucleospermat did not show any cases of allergic reactions, toxicity or complications in oncological patients.

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