scholarly journals In vitro culture and characterization of the HSP70 gene on Vanda tricolor Lindley var. Suavis ‘Queen Maxima’

2020 ◽  
Vol 28 ◽  
pp. 03004
Author(s):  
Endang Semiarti ◽  
Naufal Ghozi Adityal Perdana ◽  
Rozikin Rozikin ◽  
Febri Yuda Kurniawan
Keyword(s):  
The Netherlands ◽  
Ex Situ Conservation ◽  
Active Volcano ◽  
Ex Situ ◽  
Amino Acid Motif ◽  
Hsp70 Protein ◽  
New Generation ◽  
Special Region

Vanda tricolor Lindey var. Suavis is an endemic orchid from Mt.Merapi, Yogyakarta Special Region, Indonesia. This orchid has beautiful flowers with unique patterns of white and purple spots, fragrant and can live in the slopes of Mt.Merapi which is a very active volcano. UGM in collaboration with the Netherlands carried out ex situ conservation of the V. tricolor Merapi through the self-pollination of orchids by the Queen of the Netherlands, Queen Maxima on March 11, 2020 at the UGM campus, Yogyakarta. In honor, the new generation of crosses is named Vanda tricolor var Suavis ‘Queen Maxima’. This study aims to produce mass quantities of the orchid and characterize the HSP70 gene in it. Methods: Standard in vitro culture for Vanda on MS, NP and VW medium, isolation of plant gDNA, PCR with V. tricolor HSP70primers and sequencing of amplicon DNA. The results showed that > 90 % of V. tricolor ‘Queen Maxima’ seeds germinated well in all media and the best on VW medium. V. tricolor has the HSP70 protein with PTZ00009 super family amino acid motif that 87 % similar to the HSP70 protein from the Phalaenopsis equestris orchid, which is probably the reason V. tricolor become superior to high temperature environments.

Collection, ex situ conservation and characterization of mangaba (Hancornia speciose Gomes) germplasm in coastal lowland of Northeastern Brazil

2021 ◽  
Author(s):  
Ana Veruska Cruz da Silva ◽  
Jéssica Monalisa S. P. Oliveira ◽  
Milena Nascimento Cardoso ◽  
Ana Letícia Sirqueira Nascimento ◽  
Tássia Fernanda S. N. Soares ◽  
...  
Keyword(s):  
Ex Situ Conservation ◽  
Northeastern Brazil ◽  
Ex Situ ◽  
Coastal Lowland

scholarly journals Cryopreservation of Woody Crops: The Avocado Case

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 934
Author(s):  
Chris O’Brien ◽  
Jayeni Hiti-Bandaralage ◽  
Raquel Folgado ◽  
Alice Hayward ◽  
Sean Lahmeyer ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Ex Situ Conservation ◽  
Plant Genetic Resources ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Recalcitrant Seed ◽  
In Vitro Storage ◽  
Germplasm Collections ◽  
Seed Crops

Recent development and implementation of crop cryopreservation protocols has increased the capacity to maintain recalcitrant seeded germplasm collections via cryopreserved in vitro material. To preserve the greatest possible plant genetic resources globally for future food security and breeding programs, it is essential to integrate in situ and ex situ conservation methods into a cohesive conservation plan. In vitro storage using tissue culture and cryopreservation techniques offers promising complementary tools that can be used to promote this approach. These techniques can be employed for crops difficult or impossible to maintain in seed banks for long-term conservation. This includes woody perennial plants, recalcitrant seed crops or crops with no seeds at all and vegetatively or clonally propagated crops where seeds are not true-to-type. Many of the world’s most important crops for food, nutrition and livelihoods, are vegetatively propagated or have recalcitrant seeds. This review will look at ex situ conservation, namely field repositories and in vitro storage for some of these economically important crops, focusing on conservation strategies for avocado. To date, cultivar-specific multiplication protocols have been established for maintaining multiple avocado cultivars in tissue culture. Cryopreservation of avocado somatic embryos and somatic embryogenesis have been successful. In addition, a shoot-tip cryopreservation protocol has been developed for cryo-storage and regeneration of true-to-type clonal avocado plants.

Genetic characterization of wild swamp deer populations: ex situ conservation and forensics implications

2016 ◽  
Vol 28 (6) ◽  
pp. 965-970
Author(s):  
Ved Prakash Kumar ◽  
Anupam Shrivastwa ◽  
Parag Nigam ◽  
Dhyanendra Kumar ◽  
Surendra Prakash Goyal
Keyword(s):  
Ex Situ Conservation ◽  
Genetic Characterization ◽  
Ex Situ ◽  
Swamp Deer

scholarly journals Ex situ conservation of genetic resources of field elm (Ulmus minor Mill) and European white elm (Ulmus laevis Pall)

Genetika ◽  
2004 ◽  
Vol 36 (3) ◽  
pp. 221-227
Author(s):  
Jelena Aleksic ◽  
Sasa Orlovic
Keyword(s):  
Genetic Resources ◽  
Ex Situ Conservation ◽  
Ex Situ ◽  
In Vitro Production ◽  
Ulmus Minor ◽  
Conservation Of Genetic Resources ◽  
Ulmus Laevis ◽  
Vitro Production

Principles of the conservation of genetic resources of elms (Ulmus spp) do not differ fundamentally from the general principles accepted for the conservation of genetic resources of other common Noble Hardwoods. Efficient conservation can best be achieved through appropriate combination of in situ and ex situ methods, which have distinct advantages. Besides that, ex situ conservation is employed when emergency measures are needed for rare endangered populations and when populations are too small to be managed in situ (e.g. risks of genetic drift and inbreeding). The aim of our research is ex situ conservation of genetic resources of field elm {Ulmus minor Mill) and European white elm (Ulmus laevis Pall) through establishment of field genebanks. Sampling was conducted in one population of field elm and one population of white elm. Plant material (buds) from 8 trees of field elm and 10 trees of white elm was used for in vitro production of clones. Obtained clones will be used for establishment of field genebanks on the experimental estate of the Institute of Lowland Forestry and Environment.

Collection and characterization of wild species of Phaseolus (Fabaceae) in northeastern Mexico for ex situ conservation

2014 ◽  
Vol 148 (1) ◽  
pp. 119-127 ◽  
Author(s):  
Efrain Acosta-Diaz ◽  
Ismael Hernandez-Torres ◽  
Mario D. Amador-Ramirez ◽  
Jose S. Padilla-Ramirez ◽  
Francisco Zavala-García ◽  
...  
Keyword(s):  
Wild Species ◽  
Ex Situ Conservation ◽  
Ex Situ ◽  
Northeastern Mexico

GIS-facilitated in vitro propagation and ex situ conservation of Achillea occulta

2011 ◽  
Vol 107 (3) ◽  
pp. 531-540 ◽  
Author(s):  
Katerina Grigoriadou ◽  
Nikos Krigas ◽  
Eleni Maloupa
Keyword(s):  
In Vitro Propagation ◽  
Ex Situ Conservation ◽  
Ex Situ

Ex situ conservation through the establishment of in vitro cultivation of Prosopis limensis seeds “Huarango” from Ica, Peru

Manglar ◽  
2021 ◽  
Vol 18 (4) ◽  
pp. 427-433
Author(s):  
Héctor Javier Sánchez-Sotomayor ◽  
Alfonso Orellana-García ◽  
Indira Aurora Roel Barahona ◽  
Manuel Marín Bravo ◽  
Gilmar Peña Rojas ◽  
...  
Keyword(s):  
Ex Situ Conservation ◽  
Ex Situ ◽  
In Vitro Cultivation

scholarly journals Ex situ Conservation of Three Endemic and/or Endangered Dianthus Species

2013 ◽  
Vol 41 (1) ◽  
pp. 73 ◽  
Author(s):  
Victoria CRISTEA ◽  
Liliana JARDA ◽  
Irina HOLOBIUC
Keyword(s):  
Ex Situ Conservation ◽  
Positive Impact ◽  
Botanical Garden ◽  
Ex Situ ◽  
First Year ◽  
Genetic Uniformity ◽  
Current Context ◽  
Different Populations

Within the current context of declining biodiversity, the botanical gardens play an essential role in its conservation. Dianthus callizonus, D. glacialis ssp. gelidus and D. spiculifolius are the species that we seek to preserve in "Alexandru Borza" Botanical Garden of Cluj-Napoca (Romania). Several replicates were collected for each taxon from different populations in order to avoid the genetic uniformity. The material collected from the natural sites, was planted on a rockery, specially designed for this collection in the Botanical Garden. At the time of planting, each individual was sampled for setting up an in vitro collection and further biochemical and molecular analyses. In case of ex situ outdoor conservation of the three Dianthus species, 80.6% of the individuals collected in the field survived during the first year but the percentage decreased drastically after four years. In the case of in situ collected individuals, as well as in the case of in vitro individuals, D. spiculifolius had the best ability to acclimatize in the Botanical Garden, and D. callizonus presented the lowest number of surviving individuals. The ex vitro acclimatization of the plantlets had 80% efficiency at 10ºC, using three different substrates: soil and pearl stone mix 1/1, soil and sand mix 1/1 and pearl stone. All the three species are preserved in vitro, whereas the plantlets are acclimatized outdoors. Ex situ conservation of these species will have a positive impact on the biodiversity conservation.

scholarly journals In vitro establishment of Monstera acuminata Koch and Monstera deliciosa Liebm

2019 ◽  
Vol 68 (3) ◽  
pp. 196-204
Author(s):  
Nancy Mariel Casanova Palomeque ◽  
Vincenzo Bertolini ◽  
Leobardo Iracheta Donjuan
Keyword(s):  
Ex Situ Conservation ◽  
Leaf Explants ◽  
Ex Situ ◽  
Tween 20 ◽  
Mature Leaves ◽  
Solid Media ◽  
Monstera Deliciosa ◽  
In Vitro Establishment ◽  
Semi Solid

In this paper we present the bases for the aseptic establishment of Monstera acuminata Koch and Monstera deliciosa Liebm (Araceae) from leaves and the induction of in vitro organogenesis of M. acuminata K. from discs of stems of young shoots. Different disinfection protocols were applied to mature leaves and young shoots, from which foliar explants of 1 cm2 of both species were extracted, and stem disks approximately 1 mm of thick in M. acuminata K. The explants were established in semi-solid media with different hormonal treatments during the stage of aseptic establishment and induction of organogenesis. Disinfection with 3 % of sodium hypochlorite (NaClO) for 20 min and 50 % Murashige y Skoog medium (1962) with PPM, led to less oxidation in the leaf explants of both species. All the explants in the two treatments belonging to M. deliciosa, grown in medium added with PPM and different disinfection protocols, survived, did not present contamination and more than 80 % maintained answer’s capacity until 49 days of culture. After 35 days of culture, with disinfection in Tween 20 + Ethanol 20% + 2.5% NaClO, and sowing of the explants in the MS medium added with 1 mg/l of BAP, 0.5 mg/l of AIA and 0.1 mg/l of ANA were indueces seven new shoots of stem disks. M. deliciosa had better ability to adapt to in vitro conditions. Advances in the establishment and induction of organogenesis in native araceas for wicker production are the basis for ex situ conservation of local populations.

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