scholarly journals Assessment of strawberry varieties by anthracnose resistance gene

2021 ◽  
Vol 34 ◽  
pp. 02007
Author(s):  
Alexander Lyzhin ◽  
Irina Luk’yanchuk
Keyword(s):  
Resistance Gene ◽  
Dna Marker ◽  
Resistance Allele ◽  
Heterozygous State ◽  
Anthracnose Resistance

The results of marker-assisted identification of the Rca2 anthracnose resistance gene in promising strawberry varieties were shown. The DNA marker STS-Rca2_240, linked to the Rca2 anthracnose resistance allele was identified in varieties Albion and Aprica (F. × ananassa), and variety Dar (F. × anashata). Strawberry varieties Alpha, Bereginya, Kokinskaya Zarya, Pamyati Zubova, Rubinovy kaskad, Slavutich, Yuniol, Big King, Brilla, Joly, Rumba and Vivara are characterized by the absence of the marker STS-Rca2_240. In strawberry varieties with the identified marker STS-Rca2_240 (Dar, Albion and Aprica), the Rca2 gene can be in a dominant homozygous or heterozygous state, which makes it possible to recommend these genotypes for use in breeding for anthracnose resistance.

scholarly journals Creation of new tomato forms with fungal disease resistance genes based on marker selection

2021 ◽  
pp. 16-21
Author(s):  
I. N. Shamshin ◽  
E. V. Grosheva ◽  
M. V. Maslova ◽  
R. M. Samoilova
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Fungal Diseases ◽  
Heterozygous State ◽  
Standard Type ◽  
Marker Selection ◽  
Young Leaves ◽  
Hybrid Forms

Relevance. The presented studies are aimed at obtaining new forms of tomato with a complex of genes for resistance to fungal diseases in combination with a standard type of bush and dark coloring of fruits based on marker-mediated selection.Methodology. The biological objects of the study are varieties and hybrid forms of tomato from the collection of the Michurinsky SAU. Molecular genetic analysis was performed using the following methods. DNA extraction was carried out from young leaves using a kit for isolation of NC Sample NC manufactured by Agrodiagnostika LLC according to the manufacturer's protocol. Fermentas production kits were used for PCR. Identification of the cladosporosis resistance gene was Cf-19 performed using the DNA marker R7. The presence of a fusarious wilting resistance gene was determined by a I-2/5 marker. The amplification results were visualized by agarose gel electrophoresis.Results. During the research, a collection of varieties and hybrid forms of tomato of the Michurinsky GAU was analyzed in order to identify genes for resistance to cladosporiosis Cf-19 and fusarium wilt I-2. A total of 52 genotypes were analyzed. It was found that most samples (41 samples) are characterized by a heterozygous state of the Cf-19 gene. All indeterminant and semi-determinant forms had both alleles. Of the 23 determinant forms presented in the collection, 10 had only one allele corresponding to recessive homozygote. Among all analyzed tomato genotypes, no dominant homozygous forms were noted. The study of the collection revealed several alleles of the I-2 gene. In total, four fragments corresponding to various alleles were amplified. A total of 50 resistant genotypes have been identified in the collection. Two alleys of the I-2 gene (633/693 bp) were identified in 42 tomato samples. Four varieties are homozygous in one allele (633 bp), which determines resistance. Three varieties have a second resistance allele (566 bp). One genotype has only an allele defining susceptibility (693 bp). On the basis of molecular analysis, as well as an assessment of the type of bush and fetal color, initial forms were selected with subsequent hybridization. 67 hybrid tomato plants were obtained. Evaluation of the presence of resistance genes showed that most of the resulting hybrids are resistant to cladosporiosis and fuzariosis. This is due to the presence of dominant alleles of Cf-19 and I-2 genes in a heterozygous state. Among the resulting hybrids, plants with a bark type of bush were identified. A total of 13 such plants were obtained.Conclusion. Thus, the work carried out allowed to obtain hybrid forms of tomato combine the signs of resistance to two pathogens of fungal diseases and the stem type of the bush. These forms are planned to be used in further selection work.

Common Bean Landrace Jalo Listras Pretas Is the Source of a New Andean Anthracnose Resistance Gene

Crop Science ◽  
2009 ◽  
Vol 49 (1) ◽  
pp. 133-138 ◽  
Author(s):  
M. C. Gonçalves-Vidigal ◽  
Pedro S. Vidigal Filho ◽  
A. F. Medeiros ◽  
M. A. Pastor-Corrales
Keyword(s):  
Common Bean ◽  
Resistance Gene ◽  
Anthracnose Resistance

scholarly journals Targeted Introgression of a Wheat Stem Rust Resistance Gene by DNA Marker-Assisted Chromosome Engineering

Genetics ◽  
2011 ◽  
Vol 187 (4) ◽  
pp. 1011-1021 ◽  
Author(s):  
Zhixia Niu ◽  
Daryl L. Klindworth ◽  
Timothy L. Friesen ◽  
Shiaoman Chao ◽  
Yue Jin ◽  
...  
Keyword(s):  
Resistance Gene ◽  
Stem Rust ◽  
Rust Resistance ◽  
Dna Marker ◽  
Stem Rust Resistance ◽  
Rust Resistance Gene ◽  
Stem Rust Resistance Gene ◽  
Chromosome Engineering ◽  
Wheat Stem Rust

scholarly journals Candidate Gene Identification with SNP Marker-Based Fine Mapping of Anthracnose Resistance Gene Co-4 in Common Bean

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0139450 ◽  
Author(s):  
Andrew J. Burt ◽  
H. Manilal William ◽  
Gregory Perry ◽  
Raja Khanal ◽  
K. Peter Pauls ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Common Bean ◽  
Resistance Gene ◽  
Fine Mapping ◽  
Snp Marker ◽  
Gene Identification ◽  
Anthracnose Resistance ◽  
Candidate Gene Identification

scholarly journals Association between RAPD marker OPAS13950C and anthracnose resistance allele Co-43 of common bean

2007 ◽  
Vol 7 (1) ◽  
pp. 24-28 ◽  
Author(s):  
M.G.M. Silva ◽  
A.L. Alzate-Marin ◽  
M.A. Moreira ◽  
E.G. Barros
Keyword(s):  
Common Bean ◽  
Rapd Marker ◽  
Resistance Allele ◽  
Anthracnose Resistance

scholarly journals Analysis of strawberry genetic collection (Fragaria L.) for Rca2 and Rpfl genes with molecular markers

2018 ◽  
Vol 22 (7) ◽  
pp. 795-799 ◽  
Author(s):  
I. V. Luk’yanchuk ◽  
A. S. Lyzhin ◽  
I. I. Kozlova
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Resistance Gene ◽  
Wild Species ◽  
Root Rot ◽  
Scar Marker ◽  
Fragaria X Ananassa ◽  
Anthracnose Resistance ◽  
Monogenic Resistance ◽  
Red Stele

Strawberry (Fragaria x ananassa Duch.) varieties are susceptible to many fungal diseases. Identification of forms, carrying resistance genes, is an important stage in breeding programs leading to resistant varieties. The use of molecular markers allows to determine with high reliability the presence of the necessary genes in the genome and to identify promising forms. Some of the common strawberry's diseases, causing significant damage to strawberry plantations, are anthracnose (Colletotrichum acutatum Simmonds) and red stele root rot (Phytophthora fragariae var. fragariae Hickman). Dominant Rca2 gene is involved in monogenic resistance to C. acutatum pathogenicity group 2. Rpf1, Rpf2, Rpf3 genes are determined in monogenic resistance to red stele root rot. The purpose of this study was molecular genetic testing genotypes of genus Fragaria L. to identify carriers of Rca2 allele anthracnose resistance and Rpf1 allele red stele root rot resistance. The objects of study were the wild species of the genus Fragaria L. and strawberry varieties (Fragaria x ananassa Duch.) of different ecological and geographic origin. To assess allelic state Rca2 anthracnose resistance gene the dominant SCAR marker STS-Rca2_240 was used, was linked to the resistance gene Rca2 with a genetic distance of 2.8 cM. Rpf1 gene red stele root rot resistance was identified with the dominant SCAR marker R1A, was linked to the resistance gene Rpf1 with a genetic distance of 3.0 cM. The resistant allele of the marker STS-Rca2_240 was identified in the Laetitia variety (Rca2Rca2 or Rca2rca2 genotype), which allows us to recommend it as a promising source in breeding for anthracnose resistance. The other studied forms have homozygous recessive state of the marker STS-Rca2_240 (putative genotype rca2rca2). The resistant allele of the marker SCAR-R1A in the varieties and wild species of strawberry under study is absent, which presumably indicates their homozygous recessive genotype of Rpf1 gene (rpf1rpf1).

scholarly journals Studies on the Tobamovirus resistance of the pepper (Capsicum annuum L.) cultivar Greygo

2001 ◽  
Vol 7 (1) ◽  
Author(s):  
P. Salomon ◽  
G. Venczel
Keyword(s):  
Resistance Gene ◽  
Capsicum Annuum ◽  
Mottle Virus ◽  
Resistance Allele ◽  
Capsicum Frutescens ◽  
Pepper Mild Mottle Virus ◽  
Capsicum Annuum L

Resistance of the Hungarian pepper (Capsicum annuum L.) cultivar "Gre.ygo" to Tohamoviruses has been investigated. All plants of the population of Greygo proved to be resistant to tobacco mosaic and tomato mosaic viruses (TMV, ToMV), both represent the pepper pathotypes Po of Tohamoviruses. Individuals of Greygo, however, were found to be susceptible to pathotypes P12 and P123 of pepper mild mottle virus (PMMV). When inoculated with the XM isolate of dulcamara yellow fleck virus (DYFV, pathotype P1) the population of Greygo segregated in resistant and susceptible plants. These results as well as inoculations of the progenies of three TMV resistant plants clearly showed, that besides the resistance allele Li the cultivar Greygo possesses also an another allele. This allele, provisionally marked by L2g behaves like to the allele L2 characteristic to Capsicum frutescens cv. . Tabasco. Determination of the identity of the allele L2g to the allele L2 needs further genetic and pathological informations. Relations between the Tohamoviruses pathogenic to pepper and the alleles of the resistance gene L are outlined for the discussion.  

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