scholarly journals Estrus Synchronization Using Prostaglandin F2α (PGF2α) and Combination of PGF2α and Gonadotropin-Releasing Hormone In Ongole Crossbred

2020 ◽  
Vol 151 ◽  
pp. 01009
Author(s):  
Pudji Astuti ◽  
Claude M. Airin ◽  
Slamet Widiyanto ◽  
Sarmin Sarmin ◽  
Amelia Hana ◽  
...  
Keyword(s):  
Statistical Methods ◽  
Artificial Insemination ◽  
Luteal Phase ◽  
Prostaglandin F2α ◽  
Gonadotropin Releasing Hormone ◽  
Estrus Synchronization ◽  
Significant Difference ◽  
Estrus Detection ◽  
Female Cattle ◽  
Estrus Phase

The response of estrus can be enhanced by providing PGF2α and a combination of PGF2αGnRH so that the time of mating using Artificial Insemination (AI) can be held precisely. This study aims to compare the response of estrus synchronization using PGF2α compare to PGF2α-GnRH. Twenty female cattle Ongole Crossbreds, 4-6 years, BCS average of 3-4 were used in this research. Cattle were selected based on their luteal phase at the days of 7 to 18 by rectal examination. Then, all of the cattle were injected by cloprostenol® with a dose of 500 ug intrauterine. Ten of the animals were injected by 2.5 mL of Fertagyl® intramuscularly after 48 hours then continued observing and response estrus detection. The onset of estrus using PGF2α was detected on 75.94 ± 0.78, while 41.57 ± 28.40 hours for PGF2α-GnRH Using statistical, there was a significant difference (P<0.05). Duration of estrus phase using PGF2α was 24.65 ± 0.49 whereas PGF2α-GnRH was 10.88 ± 7.45 hours. Using statistical methods, there was a significant difference (P <0.05). It could be concluded that the use of PGF2α was more effective for the duration and intensity of estrus, and a combination of PGF2α -GnRH is more effective for stimulating estrus.

scholarly journals PSXIII-11 Altering length of the presynchronization period in a long-term progestin-based estrus synchronization protocol for timed artificial insemination of beef heifers

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 375-376
Author(s):  
Emma R Knickmeyer ◽  
Jordan M Thomas ◽  
James William C Locke ◽  
Rachael C Bonacker ◽  
Lauren Ciernia ◽  
...  
Keyword(s):  
Artificial Insemination ◽  
Reproductive Tract ◽  
Prostaglandin F2α ◽  
Estrus Synchronization ◽  
Dominant Follicle ◽  
Estrus Detection ◽  
Timed Artificial Insemination ◽  
Split Time ◽  
Synchronization Protocol

Abstract An experiment was designed to evaluate the effect of extending the presynchronization period in a progestin-based estrus synchronization protocol. Heifers were assigned to an 18 d or 14 d CIDR® treatment (1.38 g progesterone), with prostaglandin F2α (PG; 250 mg im) administered 16 d after CIDR® removal (Day 34). Heifers at two locations (location one, n = 193; location two, n = 649) were assigned to treatment based on reproductive tract score (RTS; Scale 1–5) and weight. Estrus detection aids (Estrotect®) were applied at PG and split-time artificial insemination (STAI) was performed based on estrus expression at 66 h. Heifers that expressed estrus at 66 h were inseminated and heifers that did not express estrus were inseminated at 90 h. Only heifers that failed to express estrus by 90 h received gonadotropin-releasing hormone (GnRH; 100 µg im) at AI. At location one, blood samples were collected at PG and AI (66 h or 90 h) from all heifers to determine E2 concentrations, and transrectal ovarian ultrasound was performed to detail ovarian structures on a subset of heifers (n = 73) at both time points. The proportion of heifers expressing estrus by 66 h (60%) or in total by 90 h (84%) after PG did not differ between treatments. Pregnancy rate to STAI did not differ between treatments (P = 0.3; 52%, 14 d CIDR®-PG; 50%, 18 d CIDR®-PG), or at the end of the 60 d breeding season (P = 0.2). There were no differences between treatments in mean diameter of the dominant follicle at PG (P = 0.6) or STAI (P = 0.3), nor were any differences observed between treatments in E2 concentrations at PG (P = 0.8) or STAI (P = 0.6). These data suggest that CIDR® treatment can be extended from 14 to 18 d, providing flexibility in scheduling without compromising reproductive outcomes.

scholarly journals 299 Treatment with prostaglandin F2α and an intravaginal progesterone insert in advance of gonadotropin-releasing hormone enhances response to estrus synchronization in mature beef cows

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 138-139
Author(s):  
Rachael C Bonacker ◽  
Katy S Stoecklein ◽  
Jaclyn N Ketchum ◽  
Emma R Knickmeyer ◽  
James William C Locke ◽  
...  
Keyword(s):  
Ovarian Follicle ◽  
Prostaglandin F2α ◽  
Beef Cows ◽  
Gonadotropin Releasing Hormone ◽  
Control Treatment ◽  
Estrus Synchronization ◽  
Releasing Hormone ◽  
Representative Subset ◽  
Estrus Detection ◽  
Follicle Size

Abstract An experiment was designed to evaluate the effect of treatments in advance of gonadotropin-releasing hormone (GnRH; 100μg gonadorelin) administered at the start of estrus synchronization. We hypothesized that administration of prostaglandin F2α (PG; 500μg cloprostenol) followed by treatment with an intravaginal progesterone-releasing insert (CIDR; 1.38g progesterone) would result in increased follicle size at GnRH, thereby enhancing response to GnRH and overall response to estrus synchronization. Postpartum suckled beef cows (n = 183) in two locations were assigned to one of five treatments based on age, days postpartum, and body condition score. Treatments (Figure 1) were designed as a two-by-two factorial with a control (Treatment 1). Treatments 2–5 received a CIDR insert from Day -17 to Day -10, with (Treatments 2 and 3) or without (Treatments 4 and 5) administration of PG coincident with CIDR insertion on Day -17. Coincident with GnRH administration on Day -10, CIDR inserts were either removed (Treatments 2 and 4) or remained in place until Day -3 (Treatments 3 and 5). On Day -3, estrus detection aids were applied and a representative subset of cows in each treatment were fitted with estrus detection transmitters. Blood samples were collected on Days -27, -17, -10, -3, and 0 for determination of serum estradiol and/or progesterone concentrations via radioimmunoassay. For a representative subset of cows (n = 111), transrectal ovarian ultrasound was performed to assess ovarian follicle size and presence of corpora lutea on Days -17, -10, -3, and 0. Treatment with PG and CIDR in advance of GnRH resulted in improvements (P &lt; 0.05) in several response variables, including follicle size on Day -17 and estrous response prior to AI. Results support the hypothesis that administration of PG and treatment with a CIDR for 7 d prior to GnRH improves likelihood of GnRH response and enhances response of mature cows to estrus synchronization.

PSXII-15 Evaluation of later timing of fixed-time artificial insemination for beef cows when using sex-sorted semen following the 7 & 7 Synch protocol

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 423-424
Author(s):  
Carson M Andersen ◽  
Emily G Smith ◽  
Genna M VanWye ◽  
Jordan M Thomas
Keyword(s):  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Beef Cows ◽  
Gonadotropin Releasing Hormone ◽  
Power Of Test ◽  
Releasing Hormone ◽  
Estrus Detection ◽  
To Receive ◽  
Activation Status

Abstract An experiment was designed to evaluate later timing of fixed-time artificial insemination (FTAI) with sex-sorted semen among postpartum beef cows following the 7 & 7 Synch protocol, with the hypothesis that later timing would result in increased pregnancy rates (P/AI) among cows that expressed estrus prior to FTAI. Beef cows (n = 414) were blocked based on age and days postpartum (DPP) and randomly assigned to receive FTAI at 66 or 72 h after administration of prostaglandin F2α (PG). Estrus was synchronized using the 7 & 7 Synch protocol, which consists of administration of PG (500 μg cloprostenol) and insertion of an intravaginal progesterone-releasing insert (CIDR; 1.38 g progesterone) on Day 0, gonadotropin-releasing hormone (GnRH; 100 μg gonadorelin) on Day 7, and PG coincident with CIDR removal on Day 14. Estrus detection aids (EstrotectTM) were applied to all cows on Day 14, and activation status was recorded at fixed-time artificial insemination (FTAI) on Day 17. All cows that expressed estrus prior to FTAI received sex-sorted semen (4 × 106 cells per unit; SexedULTRA 4MTM). The proportion of cows expressing estrus prior to FTAI did not differ between treatments at this power of test [66 h: 71% (146/205); 72 h: 76% (158/209)]. Additionally, P/AI of estrous cows inseminated with sex-sorted semen did not differ between treatments [66 h: 44% (90/205); 72 h: 39% (82/209)]. In conclusion, later timing of FTAI following the 7 & 7 Synch protocol failed to improve P/AI of estrous cows inseminated with sex-sorted semen.

scholarly journals Use of hormonal subdoses applied in Bai Hui acupuncture in estrus synchronization protocols for goats

2019 ◽  
Vol 40 (4) ◽  
pp. 1501 ◽  
Author(s):  
Diego Oliveira de Souza ◽  
Monna Lopes de Araújo ◽  
Carmo Emanuel Almeida Biscarde ◽  
Claudinéia da Silva Mendes ◽  
Mariana Alves de Andrade Silva ◽  
...  
Keyword(s):  
Estrus Synchronization ◽  
Reproductive Parameters ◽  
Follicular Growth ◽  
Plasma Progesterone ◽  
Significant Difference ◽  
Estrus Detection ◽  
Detection Coverage ◽  
Equine Chorionic Gonadotropin ◽  
The Cost ◽  
Synchronization Protocol

The objective of this study was to evaluate the efficacy of delivering reduced doses of hormones via the Bai Hui acupoint in estrus synchronization in goats. A total of 40 goats received intravaginal sponges with medroxyprogesterone acetate for 7 days. The goats were then randomly distributed into 5 treatment: T1 - application of 132.5 ?g of cloprostenol and 300 IU of equine chorionic gonadotropin (eCG), both by intramuscular injection (IM); T2 - application of 39.75 ?g cloprostenol at the Bai Hui acupoint, and 300 IU of eCG by IM; T3 - application of 132.5 ?g of cloprostenol by IM, and 90 IU of eCG at the Bai Hui acupoint; T4 - application of 39.75?g of cloprostenol and 90 UI of eCG, both in Bai Hui and T5 acupuncture: application of 39.75?g of cloprostenol and 90 UI of eCG, both applied in false acupoint. The goats were subjected to an estrus synchronization protocol and monitored for estrus detection, coverage and evaluation of reproductive parameters to detect entry into estrus. The data were subjected to normality tests, followed by appropriate statistical analyses of each variable. There was no significant difference (P > 0.05) in the percentage of animals in estrus (95.00 ± 11.18%), interval between sponge removal and beginning of estrus (49.72 ± 8.93 h), interval between sponge removal and end of estrus (76.84 ± 11.98 h), duration of estrus (27.08 ± 8.68 h), size of the largest follicle (6.82 ± 0.44 mm), interval between sponge removal and ovulation (78.28 ± 10.82 h), time from ovarian onset to estrus (28.52 ± 5.44 h), follicular growth rate (0.86 ± 0.29 mm/day), number of ovulations (1.32 ± 0.23), plasma progesterone concentration at 7 days after ovulation (10.28 ± 1.65 ng.mL-1), and gestation rate at 30 days after the beginning of estrus (75 ± 12.5%). However, the cost of the synchronization protocol per animal was 43.42% lower in treatments 4 and 5 (30% of the doses) than in treatment 1 (100% of the dose). Ovulation and estrus were efficiently synchronized with the use of 39.75 ?g of sodium cloprostenol and 90 UI of eCG, applied at the Bai Hui acupoint or at a false acupoint.

Estrous synchronization and fertility of cross-bred dairy cows using prostaglandin F2α and gonadotropin releasing hormone

2021 ◽  
pp. 171-175
Author(s):  
Abubakr O. Ismail ◽  
◽  
Erneo B. Ochi ◽  
Ambrose S. Jubara ◽  
Bakri Ahmed A/Rahim ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Artificial Insemination ◽  
Prostaglandin F2α ◽  
Fixed Time ◽  
Gonadotropin Releasing Hormone ◽  
Pregnancy Rates ◽  
Releasing Hormone ◽  
Treatment Groups ◽  
Estrous Synchronization ◽  
Descriptive Statistical Analysis

A comparative hormonal study of prostaglandin, gonadotropin releasing hormone and their combination was conducted on 30 randomly selected cross-bred dairy cows of SEMEX project for artificial insemination in Hillat Kuku, Khartoum North, Sudan. The study attempts to determine the response of the animals to hormonal induction and synchronization of estrous as well as fertility following fixed time artificial insemination. Three treatment groups of 10 cows each were undertaken. Groups 1, 2 and 3 involved administration of prostaglandin F2α (PGF2α), gonadotropin releasing hormone (GnRH) and a combination of PGF2α + GnRH, respectively. Descriptive statistical analysis was performed. The results revealed that all the three protocols induced and synchronized estrous were almost synchronous in all the treated groups. However, protocol 3 revealed a significantly (p<0.05) better result of 70% pregnancy rate compared to other two protocols that equally provided 50% pregnancy rates. The study resolves that protocol 3 be highly recommended to change the mindset of farmers about the spread of reproductive technology in Sudan. Protocols 1 and 2 provided acceptable pregnancy rates that can be enhanced with the improvement of management.

Synchronization of oestrus and subsequent fertility in cattle, using the prostaglandin F2α analogue, ICI 80996 (cloprostenol)

1976 ◽  
Vol 16 (81) ◽  
pp. 437 ◽  
Author(s):  
H Hearnshaw
Keyword(s):  
Artificial Insemination ◽  
Luteal Phase ◽  
Prostaglandin F2α ◽  
Optimum Time ◽  
Subcutaneous Injections ◽  
Late Luteal Phase ◽  
Initial Injection ◽  
Subsequent Fertility ◽  
Subsequent Cycle ◽  
Cycle Lengths

The prostaglandin analogue, ICI 80996 (cloprostenol), was administered subcutaneously to synchronize oestrus in cattle. One injection, or two injections 12 days apart, of 400 pg gave optimal results (78 and 72 per cent synchronized respectively) for the Hereford cross heifers used. Heifers treated with cloprostenol from days one to five of the cycle, did not respond to the first injection, but did so to a second injection given 12 days later. A greater percentage of heifers, treated in the mid or late luteal phase of the cycle, exhibited oestrus after the first injection of cloprostenol than after the second injection. The percentage of these heifers showing induced oestrus varied from 44 to 100 per cent. There was a peak in the incidence of oestrus between 49 and 72 hours after injection but the distribution ranged from 24 to 120 hours. Peripheral blood progesterone concentrations, palpation of the ovaries and subsequent cycle lengths, indicated that the cloprostenol had no adverse effects in the majority of heifers. However, about 30 per cent of the heifers that responded to an initial injection of cloprostenol given in the mid luteal phase of the cycle failed to respond to a second injection. Some of these heifers had abnormal progesterone profiles and subsequent reproductive patterns. Fertility, following one or two subcutaneous injections of cloprostenol and artificial insemination at the set times of 72 and 96 hours, was 30 and 46 per cent respectively. However, if only heifers inseminated at the optimum time were considered, fertility at pregnancy diagnosis (73 and 65 per cent) and calving (53 and 57 per cent) was acceptable.

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