scholarly journals Clonal micropropagation of Thymus vulgaris L.

2020 ◽  
Vol 224 ◽  
pp. 04001
Author(s):  
A Sh Tevfik ◽  
N. A. Yegorova
Keyword(s):  
Plant Material ◽  
Culture Medium ◽  
Culture Media ◽  
Morphometric Parameters ◽  
Thymus Vulgaris ◽  
Ms Medium ◽  
Ex Vitro ◽  
Clonal Micropropagation ◽  
Propagation Stage

Thymus vulgaris L. is one of the widely known spicy aromatic and medicinal plants. Thyme plant material is widely used in medicine, cooking and perfumery. To increase the efficiency of breeding and seed production, it is necessary to develop biotechnological techniques, in particular, clonal micropropagation. The aim of the research is to optimize the composition of culture media for the main stages of propagation in vitro and to select adaptation ex vitro conditions for the development of Thymus vulgaris. clonal micropropagation. The article presents the results of studies of explant morphometric parameters cultivated on 20 variants of culture media at firstsecond stages of micropropagation. It was found that the optimal culture medium at the introduction stage is MS medium with 1.0 mg/l Kin and 1.0 mg/l GA3, on which, on average, 2.2 microshoots per explant with a length of 1.9 cm were obtained. Both high vitrification rate of microshoots and formation of small shoots (0.6-0.9 cm) were observed on media supplemented with BAP or TDZ. The most effective culture medium at the proper propagation stage is MS with 1.0 mg/l Kin, on which 4.6 shoots per explant and the multiplication index 12.8 were obtained. It is advisable to root microshoots at the 3rd stage of micropropagation on MS culture medium supplemented with 1.0 mg/l IBA or 1.0 mg/l IAA. It has been shown that it is possible to obtain high plant survival rate (89.5%) during adaptation ex vitro, using a substrate consisting of peat and perlite (1:1).

scholarly journals Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

2021 ◽  
Vol 4 (46) ◽  
pp. 17-17
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Ms Medium ◽  
Organic Additives ◽  
Clonal Micropropagation ◽  
Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

scholarly journals Multiplicação de anador (Justicia pectoralis) in vitro

2016 ◽  
Vol 11 (3) ◽  
pp. 159 ◽  
Author(s):  
Rômulo Magno Oliveira Freitas ◽  
Narjara Walessa Nogueira ◽  
Sidney Carlos Praxedes
Keyword(s):  
Plant Material ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Statistical Design ◽  
The Other ◽  
Nodal Segments ◽  
Ms Medium ◽  
Number Of Leaves

<p>O trabalho teve como objetivo desenvolver um protocolo de micropropagação de segmentos nodais de anador (<em>Justicia pectoralis</em>). Para isso foram realizados dois experimentos. O delineamento estatístico utilizado foi o inteiramente casualizado, com 15 repetições. Os segmentos de <em>J. pectoralis</em>, após desinfestados, foram cultivados em meio MS durante 30 dias. No primeiro experimento, esse material foi repicado em três meios de cultura (MS, WPM e B5) e após 77 dias foram avaliados comprimento de plântula, número de raízes, número de folhas e o número de segmentos nodais. Para o segundo experimento foram testadas duas citocininas (BAP e Cinetina) nas seguintes dosagens 0,0; 0,5; 5,0 e 20,0 mM. Aos 60 dias após a repicagem foram avaliadas as seguintes características: números de folhas, número de raízes e número de explantes por planta. O meio MS foi o que apresentou maior comprimento de plântula. As demais variáveis não diferiram entre os meios utilizados. Por isso o meio MS foi utilizado para o segundo experimento onde se verificou que a utilização de BAP proporcionou maior número de folhas e de explantes quando submetido à concentração de 20 mM. Dessa forma, para multiplicação de seg <em>Justicia pectoralis</em>, recomenda-se a utilização de meio MS com adição de 20mM de BAP.</p><p align="center"><strong><em>In vitro propagation of </em></strong><em>Justicia pectoralis<strong></strong></em></p><p><strong>Abstract</strong><strong>: </strong>The study aimed to establish a micropropagation protocol for <em>Justicia pectoralis</em> nodal segments. Two experiments were conducted. The statistical design was the completely randomized with 15 repetitions. After disinfestation, the segments of <em>J. pectoralis</em> were inoculated in the MS culture medium for 30 days. In the first experiment, the plant material was transferred to three culture media (MS, WPM and B5). The length of seedlings, number of roots, number of leaves, and number of nodal segments were evaluated at 77 days after transferring. In the second experiment two cytokinins (BAP and Kinetin) were tested in the following concentrations: 0.0; 0.5; 5.0 and 20.0 mM. At 60 days after transplanting the number of leaves, number of roots and number of explants per plant were evaluated. The MS medium induced the highest length of seedlings, but there was no effect for the other variables. Therefore, this medium was used for the second experiment, when it was found that BAP induced a larger number of leaves and explants when applied at 20 mM. Therefore, for multiplying <em>J. pectoralis</em> nodal segments we recommend the use of MS medium with 20 mM BAP.</p>

scholarly journals Culture medium for mint micropropagation in vitro

2020 ◽  
Author(s):  
N.A. Yegorova ◽  
◽  
M.S. Zagorskaya ◽  
O.V. Yakimova ◽  
◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Culture Medium ◽  
Medium Composition ◽  
Multiplication Rate ◽  
Ms Medium ◽  
Second Stage ◽  
Clonal Micropropagation ◽  
Propagation Technique

The influence of the culture medium composition on the development of explants at the second stage of clonal micropropagation of mint (Mentha canadensis L. K59(4n)) was studied in order to improve the in vitro propagation technique. It was shown that the maximum multiplication rate (11.5) was provided by MS medium supplemented with BAP (1.0 mg/L), IAA (0.5 mg/L) and 2% sucrose.

scholarly journals Micropropagation protocol for the wild Brazilian greenberry (Rubus erythroclados)

2018 ◽  
Vol 12 (2) ◽  
pp. 405-415
Author(s):  
Paulo Mauricio Centenaro Bueno ◽  
Luiz Antonio Biasi ◽  
Mauro Brasil Dias Tofanelli
Keyword(s):  
Culture Medium ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Ms Medium ◽  
Ex Vitro ◽  
Indolebutyric Acid ◽  
Simple Protocol ◽  
Growth Room ◽  
In Vitro Shoot Proliferation

This study presents the first micropropagation protocol for greenberry (Rubus erythroclados), a wild Brazilian species with edible green fruits. In the in vitro multiplication stage, three concentrations of benzyladenine (BA) were tested (0, 5 and 10 μM), combined with three concentrations of indolebutyric acid (IBA) (0, 3 and 6 μM) in two subsequent subcultures. In the rooting stage, in and ex vitro rooting were compared after pulse treatment of the microcutting for 10 seconds in IBA (0, 2.46, 4.92 and 7.38 mM). For the in vitro trial, the microcuttings were maintained in glass bottles with an MS medium under controlled conditions inside a growth room. For the ex vitro trial, the microcuttings were planted in styrofoam containers with vermiculite and maintained inside a greenhouse with an intermittent mist system. R. erythroclados multiplication was obtained with the addition of BA to the culture medium, while IBA reduced the shoot proliferation and increased mortality. The ex vitro rooting showed the best results, reaching 95.8% for rooted and acclimatizated plants without IBA. An efficient and simple protocol can be used for R. erythroclados micropropagation with 5 μM BA for in vitro shoot proliferation and ex vitro rooting of microcuttings with intermittent misting.

scholarly journals Optimization of clonal micropropagation of Chrysanthemum

2019 ◽  
Vol 9 (4) ◽  
pp. 676-678
Author(s):  
I. D. Borodulina ◽  
A. Trufanova ◽  
G. Shevchenko ◽  
G. Sokolova ◽  
T. Plaksina
Keyword(s):  
Growth Regulators ◽  
Traditional Method ◽  
Proliferative Activity ◽  
Culture Medium ◽  
Ms Medium ◽  
Mineral Salts ◽  
Clonal Micropropagation ◽  
Murashige Skoog ◽  
High Proliferative Activity

Micropropagation of Chrysanthemum is an alternative to the traditional method of reproduction. Thanks to this method, the Chrysanthemum reproduction time is reduced to 3-4 months. For clonal micropropagation, sterile microshoots of Chrysanthemums of the varieties Snow White, Stranger, and Baltica White were used. At the stage of the micropropagation, the Murashige-Skoog (MS) medium with the full and half composition of mineral salts and growth regulators (kinetin, 6-benzylaminopurine, β-indolylacetic acid) were used. A universal culture medium for clonal micropropagation of all varieties of Chrysanthemum and optimal mediums, taking into account variety-specificity were established. It was noted that under in vitro conditions, high proliferative activity was observed in Neznakomka variety.

scholarly journals Optimization Studies of Culture Media for In-Vitro Clonal Micropropagation of New Grape Varieties

2021 ◽  
Author(s):  
Abdulmalik Batukaev ◽  
Eliza Sobralieva ◽  
Diana Palaeva
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
In Vitro Regeneration ◽  
Mineral Salts ◽  
Additional Increase ◽  
Shoot Bud ◽  
Clonal Micropropagation ◽  
Aseptic Culture ◽  
Grape Varieties

This article describes the effect of Gautheret, White, Heller and Murashige & Skoog mineral salts during in-vitro clonal micropropagation of new grape varieties. The optimal mineral compositions of the culture medium that support the in-vitro regeneration of isolated grape explants were identified. The grapes that were studied were the Bart and Augustine varieties. Primary grape explants were cultivated for 30 days in a non-transplanted culture. Increased regenerative activity was observed in the Murashige & Skoog and White media. Increased haemogenesis occurred and shoots regenerated. The addition of cytokinin 6-BAP to the medium for obtaining aseptic culture led to an increase in the frequency of shoot-bud production by 5 to 6 times, depending on the type of medium. Combining 6-BAP with the auxin NAA provided an additional increase in the frequency of shoot-bud production, but to a lesser extent. Adding growth regulators to the culture medium also reduced the frequency of explant necrosis. Keywords: grapes, mineral salts, culture medium, microclonal propagation, in-vitro, cytokinins, auxins

scholarly journals Micropropagation of some grape varieties in Kazakhstan

2020 ◽  
Vol 25 ◽  
pp. 05003
Author(s):  
Saule Kazybayeva ◽  
Irina Kovalchuk ◽  
Timur Turdiyev ◽  
Shokan Kulshanov ◽  
Laura Azhitayeva
Keyword(s):  
Mineral Nutrition ◽  
Culture Media ◽  
Agar Medium ◽  
Ms Medium ◽  
Clonal Micropropagation ◽  
Grape Varieties

The article shows the improvement of the process of initiation into in vitro the culture and the clonal micropropagation of grape varieties. The optimal culture media for the initiation and cloning of grapes in vitro have been selected. During initiation on Murashige and Skoog, agar medium with ½ or ¾ concentration of macroand micronutrients and hormones (0.5 mg/l BAP and 0.1 mg/l IBA) is optimal. For micropropagation is suitable MS medium modified by some elements of mineral nutrition: 825 mg/l NH4NO3, 166 mg/l CaCl2, 15 mg/l ferrum chelate; best hormonal composition depends on variety: a) 0.5-1 mg/l BAP and 0.1-0.5 mg/l IBA; b) 0.5 mg/l 2-iP and 0.5 mg/l GA3.

scholarly journals In vitro Dendrobium nobile plant growth and rooting in different sucrose concentrations

2004 ◽  
Vol 22 (4) ◽  
pp. 780-783 ◽  
Author(s):  
Ricardo T. de Faria ◽  
Fabiana N. Rodrigues ◽  
Luciana do V.R. Oliveira ◽  
Cláudio Müller
Keyword(s):  
Plant Growth ◽  
Culture Medium ◽  
Culture Media ◽  
Sucrose Concentration ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Dendrobium Nobile ◽  
Modified Ms Medium ◽  
Dendrobium Nobile Lindl

Sucrose is a very important component in in vitro culture media, serving as a source of carbon and energy. In this paper, the rooting and in vitro growth of Dendrobium nobile Lindl (Orchidaceae) were studied using different sucrose concentrations (0 g L-1; 5 g L-1; 10 g L-1; 20 g L-1; 30 g L-1 and 60 g L-1), in a modified MS medium containing half the regular concentration of macronutrients at pH 5.8. Greater increases in plant height (4.21±0.6 cm) and high seedling multiplication (1:4) were observed in the 60 g L-1 sucrose treatment, even without the addition of plant hormones. Sucrose concentration in the culture medium did not influence in vitro plant rooting.

scholarly journals Effects of Different Paclobutrazol-Cultar Concentrations on the Micropropagation of Sugarcane (Saccharum Officinarum) Variety CPCL99-4455

2018 ◽  
Vol 7 ◽  
pp. 1011-1018 ◽  
Author(s):  
Dion Daniels ◽  
Nidia Panti ◽  
David Guerra ◽  
Stephen Williams
Keyword(s):  
Growth And Development ◽  
Culture Medium ◽  
Culture Media ◽  
Saccharum Officinarum ◽  
Average Height ◽  
Ex Vitro ◽  
Agricultural Exports ◽  
Multiplication Coefficient ◽  
Important Cash Crop

Sugarcane (Saccharum officinarum), being an important cash crop in Belize, accounts for 60% of agricultural exports providing employment for thousands of Belizeans. This research was carried out to determine the effects of different Paclobutrazol (PBZ-Cultar) concentrations used in the culture media on in vitro multiplication of sugarcane variety CPCL99-4455. Three PBZ-Cultar concentrations were tested and compared with the control. The parameters evaluated to determine the effects of PBZ-Cultar were average height, number of dead leaves and multiplication coefficient. The plants from this experiment were planted in the acclimatization phase to determine if the use of PBZ-Cultar had any effect of the growth and development of the plants ex vitro. The results revealed that the culture medium supplemented with 0.08% PBZ-Cultar concentration had the best results both in the multiplication phase as well as the acclimatization phase.

scholarly journals RESPONSE OF Cattleya forbesii ORCHID TO INCREASING SILICON CONCENTRATIONS IN VITRO

2016 ◽  
Vol 29 (1) ◽  
pp. 18-24 ◽  
Author(s):  
RONAN CARLOS COLOMBO ◽  
VANESSA FAVETTA ◽  
RICARDO TADEU DE FARIA ◽  
FELIPE ARANHA DE ANDRADE ◽  
VANDERLI MARINO MELEM
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Average Length ◽  
Ms Medium ◽  
In Vitro Growth ◽  
Shoot Height ◽  
Leaf Tissues ◽  
Number Of Leaves ◽  
Scanning Electron

ABSTRACT: Addition of Silicon (Si) to culture media has been shown to improve the development of seedlings grown in vitro, and to reduce losses during the acclimatization phase. The objective of this study was to evaluate the in vitro growth of Cattleya forbesii (Orchidaceae) in MS medium containing five different concentrations of SiO2 (0.0, 0.5, 1.0, 1.5, and 2.0 g·L−1). At day 200, the following variables were measured: number of roots, average length of the root system, leaf area, number of leaves and shoots, shoot height, fresh and dry masses of roots and shoots, water content of roots and shoots, and pH of the culture medium. Most variables decreased as the concentration of Si increased, reducing the in vitro vegetative growth of C. forbesii. Accumulation of Si in leaf tissues was detected by scanning electron microscopy, confirming uptake by plants. The Si source and concentrations tested showed no beneficial effect on in vitro growth of C. forbesii.

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