scholarly journals MALDI-TOF mass spectrometry: a new tool for rapid identification of cercariae (Trematoda, Digenea)

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 11 ◽  
Author(s):  
Antoine Huguenin ◽  
Jérôme Depaquit ◽  
Isabelle Villena ◽  
Hubert Ferté

Identification of cercariae was long based on morphological and morphometric features, but these approaches remain difficult to implement and require skills that have now become rare. Molecular tools have become the reference even though they remain relatively time-consuming and expensive. We propose a new approach for the identification of cercariae using MALDI-TOF mass spectrometry. Snails of different genera (Radix, Lymnaea, Stagnicola, Planorbis, and Anisus) were collected in the field to perform emitting tests in the laboratory. The cercariae they emitted (Trichobilharzia anseri, Diplostomum pseudospathaceum, Alaria alata, Echinostoma revolutum, Petasiger phalacrocoracis, Tylodelphys sp., Australapatemon sp., Cotylurus sp., Posthodiplostomum sp., Parastrigea sp., Echinoparyphium sp. and Plagiorchis sp.) were characterized by sequencing the D2, ITS2 and ITS1 domains of rDNA, and by amplification using specific Alaria alata primers. A sample of each specimen, either fresh or stored in ethanol, was subjected to a simple preparation protocol for MALDI-TOF analysis. The main spectral profiles were analyzed by Hierarchical Clustering Analysis. Likewise, the haplotypes were analyzed using the maximum likelihood method. Analytical performance and the log-score value (LSV) cut-off for species identification were then assessed by blind testing. The clusters obtained by both techniques were congruent, allowing identification at a species level. MALDI-TOF enables identification at an LSV cut-off of 1.7 without false-positives; however, it requires more data on closely related species. The development of a “high throughput” identification system for all types of cercariae would be of considerable interest in epidemiological surveys of trematode infections.

2014 ◽  
Vol 226 (02) ◽  
pp. 68-71 ◽  
Author(s):  
M. Artacho-Reinoso ◽  
P. Olbrich ◽  
P. Solano-Paéz ◽  
P. Ybot-Gonzalez ◽  
J. Lepe ◽  
...  

2016 ◽  
Vol 8 (1) ◽  
Author(s):  
Luigi Principe ◽  
Silvia Bracco ◽  
Carola Mauri ◽  
Silvia Tonolo ◽  
Beatrice Pini ◽  
...  

<em>Erysipelothrix</em> <em>rhusiopathiae</em> is a Gram-positive bacillus that is infrequently responsible for infections in humans. Three forms have been classified: a localized cutaneous form (erysipeloid) caused by traumatic penetration of <em>E.</em> <em>rhusiopathiae</em>, a generalized cutaneous form and a septicemic form. The latter type of disease has been previously associated with a high incidence of endocarditis. Here we report a case of <em>E. rhusiopathiae</em> bacteremia in a 74- year-old man, probably started from an erysipeloid form, in which endocarditis did not develop. This case presents some particular and uncommon features: i) no correlation with animal source; ii) correlation between bacteremia and erysipeloid lesion; iii) absence of endocarditis. MALDI-TOF mass spectrometry allowed to obtain a rapid identification (within 4 hours from bottle positivity) of <em>E. rhusiopathiae</em>. Together with direct antimicrobial susceptibility testing, this approach could improve the rate of appropriate therapy for bloodstream infections due to this fastidious pathogen.


2018 ◽  
Vol 19 (2) ◽  
pp. 394-407 ◽  
Author(s):  
Nikola HRICÁKOVÁ ◽  
Juraj MEDO ◽  
Lukáš HLEBA ◽  
Marek BARTA ◽  
Jana MAKOVÁ

2015 ◽  
Vol 109 ◽  
pp. 93-105 ◽  
Author(s):  
Ashutosh Panda ◽  
Anup K. Ghosh ◽  
Bijay R. Mirdha ◽  
Immaculata Xess ◽  
Saikat Paul ◽  
...  

2010 ◽  
Vol 33 (9) ◽  
pp. 568-574 ◽  
Author(s):  
Llinos G. Harris ◽  
Khalid El-Bouri ◽  
Stuart Johnston ◽  
Eugene Rees ◽  
Lars Frommelt ◽  
...  

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