scholarly journals Cholestatic expression pattern of sinusoidal and canalicular organic anion transport systems in primary cultured rat hepatocytes

Hepatology ◽  
2001 ◽  
Vol 33 (4) ◽  
pp. 776-782 ◽  
Author(s):  
S Rippin
Keyword(s):  
Expression Pattern ◽  
Organic Anion ◽  
Rat Hepatocytes ◽  
Anion Transport ◽  
Transport Systems ◽  
Organic Anion Transport ◽  
Primary Cultured Rat Hepatocytes ◽  
Cultured Rat Hepatocytes

scholarly journals Influence of Cl- on organic anion transport in short-term cultured rat hepatocytes and isolated perfused rat liver.

1987 ◽  
Vol 79 (4) ◽  
pp. 1259-1268 ◽  
Author(s):  
A W Wolkoff ◽  
A C Samuelson ◽  
K L Johansen ◽  
R Nakata ◽  
D M Withers ◽  
...  
Keyword(s):  
Rat Liver ◽  
Organic Anion ◽  
Rat Hepatocytes ◽  
Anion Transport ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Organic Anion Transport ◽  
Short Term ◽  
Cultured Rat Hepatocytes

Redistribution of canalicular organic anion transport activity in isolated and cultured rat hepatocytes

Hepatology ◽  
1995 ◽  
Vol 21 (6) ◽  
pp. 1649-1657 ◽  
Author(s):  
Han Roelofsen ◽  
Conny T. M. Bakker ◽  
Berry Schoemaker ◽  
Marc Heijn ◽  
Peter L. M. Jansen ◽  
...  
Keyword(s):  
Organic Anion ◽  
Rat Hepatocytes ◽  
Anion Transport ◽  
Organic Anion Transport ◽  
Transport Activity ◽  
Cultured Rat Hepatocytes ◽  
Anion Transport Activity

scholarly journals Down-Regulation of Organic Anion Transporter 2 mRNA Expression by Nitric Oxide in Primary Cultured Rat Hepatocytes

IUBMB Life ◽  
2002 ◽  
Vol 54 (3) ◽  
pp. 129-135 ◽  
Author(s):  
Seok Ho Cha ◽  
Hong Pyo Kim ◽  
Nam-Hee Jung ◽  
Woon Ki Lee ◽  
Ju Young Kim ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mrna Expression ◽  
Organic Anion ◽  
Rat Hepatocytes ◽  
Organic Anion Transporter ◽  
Down Regulation ◽  
Primary Cultured Rat Hepatocytes ◽  
Anion Transporter ◽  
Cultured Rat Hepatocytes

scholarly journals Extracellular ATP4- modulates organic anion transport by rat hepatocytes

1993 ◽  
Vol 268 (21) ◽  
pp. 15399-15404
Author(s):  
C.G. Campbell ◽  
D.C. Spray ◽  
A.W. Wolkoff
Keyword(s):  
Organic Anion ◽  
Rat Hepatocytes ◽  
Anion Transport ◽  
Organic Anion Transport

The Use of Primary Cultured Rat Hepatocytes for the Assessment of Xenobiotic Effects on Biotransformation

1991 ◽  
Vol 19 (2) ◽  
pp. 209-213
Author(s):  
Gabi Schepers ◽  
Christiane Aschmann ◽  
Sabine Mörchel
Keyword(s):  
Cell Viability ◽  
Rat Hepatocytes ◽  
Standard Test ◽  
In Vitro Test ◽  
Chlorinated Phenols ◽  
Test Protocol ◽  
Primary Cultured Rat Hepatocytes ◽  
Cultured Rat Hepatocytes ◽  
Different Response

An in vitro test protocol is reported, which, using primary cultured rat hepatocytes, allows for the screening of xenobiotic effects on biotransformation as well as on basal cellular functions. O-Deethylation of 7-ethoxycoumarin (7-EC) and subsequent conjugation of the metabolite 7-hydroxycoumarin (7-HC) with sulphate or glucuronic acid are determined, as representative parameters for the hepatic biotransformation. Cell viability is examined by measuring cellular ATP content and leakage of lactate dehydrogenase. With respect to immediate and delayed effects on biotransformation reactions, the standard test protocol includes exposure to xenobiotics for 1, 24 and 48 hours. Different response patterns could be demonstrated for the solvents dimethylformamide (DMF) and dimethylsulphoxide (DMSO), and the chlorinated phenols, pentachlorophenol (PCP) and hexachlorophene (HCP), which are known to uncouple mitochondrial respiration. Short-term incubation with the solvents resulted in decreased 7-EC- O-deethylation without signs of cytotoxicity. PCP and HCP inhibited 7-EC- O-deethylation and 7-HC-conjugation, affecting sulphate and glucuronide formation differently. 24-hour exposures to PCP and HCP resulted in decreased 7-ethoxycoumarin- O-deethylase activity, which correlated with diminished cell viability, while DMSO and DMF enhanced 7-EC- O-deethylation at sub-cytotoxic concentrations. After exposure for 48 hours to the solvents, enzyme induction was even more pronounced.

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