O-8: Localization of the class I disulfide bonds of the human insulin receptor and the role of class I disulfide bonds in insulin receptor signal transduction

2009 ◽  
Vol 104 (S 02) ◽  
pp. 14-14
Author(s):  
Kuanghui Lu ◽  
Guido Guidotti
Keyword(s):  
Signal Transduction ◽  
Insulin Receptor ◽  
Human Insulin ◽  
Disulfide Bonds ◽  
Class I ◽  
Receptor Signal ◽  
Human Insulin Receptor

scholarly journals Identification of the cysteine residues involved in the class I disulfide bonds of the human insulin receptor: properties of insulin receptor monomers.

1996 ◽  
Vol 7 (5) ◽  
pp. 679-691 ◽  
Author(s):  
K Lu ◽  
G Guidotti
Keyword(s):  
Insulin Receptor ◽  
Human Insulin ◽  
Disulfide Bonds ◽  
Insulin Binding ◽  
Site Directed Mutagenesis ◽  
Class I ◽  
Cysteine Residues ◽  
Human Insulin Receptor ◽  
Alpha Beta ◽  
Receptor Dimers

The cysteine residues involved in the class I disulfide bonds between the alpha subunits in the (alpha beta)2 dimer of the human insulin receptor have been identified by labeling with N-ethylmaleimide and by site-directed mutagenesis. Both cysteine 524 and cysteine 682 form interchain disulfide bonds; their conversion to serine residues results in the absence of receptor dimers and the presence of alpha beta monomers. The receptor monomers have a slightly lower affinity for insulin than the native receptor dimers. Insulin binding to the receptor monomers promotes their dimerization in the plasma membrane; at nanomolar concentrations of receptor, both unliganded and liganded receptors are monomers. Receptor monomers are stimulated by insulin to autophosphorylate and to phosphorylate exogenous subtrates with the same efficiency as the receptor dimers. The conclusion is that receptor dimerization is not required to activate the tyrosine kinase activity of the insulin receptor.

scholarly journals Insulin Signal Transduction by a Mutant Human Insulin Receptor Lacking the NPEY Sequence

1997 ◽  
Vol 272 (36) ◽  
pp. 22884-22890 ◽  
Author(s):  
Paulos Berhanu ◽  
Celia Anderson ◽  
Matt Hickman ◽  
Theodore P. Ciaraldi
Keyword(s):  
Signal Transduction ◽  
Insulin Receptor ◽  
Human Insulin ◽  
Insulin Signal Transduction ◽  
Insulin Signal ◽  
Human Insulin Receptor

scholarly journals The Disulfide Bonds in the C-terminal Domains of the Human Insulin Receptor Ectodomain

1997 ◽  
Vol 272 (47) ◽  
pp. 29460-29467 ◽  
Author(s):  
Lindsay G. Sparrow ◽  
Neil M. McKern ◽  
Jeff J. Gorman ◽  
Phillip M. Strike ◽  
Christine P. Robinson ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
Human Insulin ◽  
Disulfide Bonds ◽  
Human Insulin Receptor ◽  
Terminal Domains
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