Development of low-Gly m Bd 30K(P34) allergen breeding lines using molecular marker in soybean

Genetic diversity can be measured by several criteria, including phenotype, pedigree, allelic diversity at marker loci, and allelic diversity at loci controlling phenotypes of interest. Abundance, high level of polymorphism, and ease of genotyping make simple sequence repeats (SSRs) an excellent molecular marker system for genetics diversity analyses. In this study, we used a set of mapped SSRs to survey three representative groups of barley germplasm: a sample of crop progenitor (Hordeum vulgare subsp. spontaneum) accessions, a group of mapping population parents, and a group of varieties and elite breeding lines. The objectives were to determine (i) how informative SSRs are in these three sets of barley germplasm resources and (ii) the utility of SSRs in classifying barley germplasm. A total of 687 alleles were identified at 42 SSR loci in 147 genotypes. The number of alleles per locus ranged from 4 to 31, with an average of 16.3. Crop progenitors averaged 10.3 alleles per SSR locus, mapping population parents 8.3 alleles per SSR locus, and elite breeding lines 5.8 alleles per SSR locus. There were many exclusive (unique) alleles. The polymorphism information content values for the SSRs ranged from 0.08 to 0.94. The cluster analysis indicates a high level of diversity within the crop progenitors accessions and within the mapping population parents. It also shows a lower level of diversity within the elite breeding germplasm. Our results demonstrate that this set of SSRs was highly informative and was useful in generating a meaningful classification of the germplasm that we sampled. Our long-term goal is to determine the utility of molecular marker diversity as a tool for gene discovery and efficient use of germplasm.Key words: Hordeum vulgare subsp. vulgare, Hordeum vulgare subsp. spontaneum, SSR, genetic diversity, germplasm.

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Development of New Drought Tolerant Breeding Lines for Vietnam Using Marker-Assisted Backcrossing

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.59.1 ◽

2016 ◽

Vol 59 ◽

pp. 1-13 ◽

Cited By ~ 4

Author(s):

Pham Thi Thu Ha ◽

Do Tan Khang ◽

Phung Thi Tuyen ◽

Tran Bao Toan ◽

Nguyen Ngoc Huong ◽

...

Keyword(s):

Drought Stress ◽

Molecular Marker ◽

Mekong Delta ◽

High Yield ◽

Rice Varieties ◽

Breeding Lines ◽

Drought Tolerant ◽

High Yielding Varieties ◽

Marker Assisted Backcrossing ◽

Recipient Parent

Development of drought tolerant high-yielding varieties is essential because increased areas are subject to drought in the Mekong delta, Vietnam. The purpose of this experiment was developed using IR75499-73-1-B as drought tolerant donor and OMCS2000 as a recipient parent basis of a phenotypic and molecular marker for BC2F2 generation. Seven markers (RM219, RM201 RM105, RM23602, RM23877, RM24103 and RM328) were used for a idifying to drought tolerant. Primer RM23877 detected the highest number of lines as homozygous donor alleles (11 lines), followed by RM105 and RM201 (9 lines). The drought gene was introgressed into the new breeding lines. The plant height, number of tillers, and filled grain had positive correlation with yield/hill under drought stress. The lines BC2F2-45 and BC2F2-54 developed as drought tolerant, and gave high yield. This is an opportunity to improve breeding for high yield and drought tolerant rice varieties in Vietnam.

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The Role of Molecular Marker in Development of Plant Genetic Resources

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.955-959.855 ◽

2014 ◽

Vol 955-959 ◽

pp. 855-858

Author(s):

Zhou Xuan ◽

Hong Dao Zhang ◽

Zheng Hong Li ◽

Cheng Zhang ◽

Ji Lin Li ◽

...

Keyword(s):

Molecular Markers ◽

Molecular Marker ◽

Genetic Resources ◽

Molecular Basis ◽

Plant Genetic Resources ◽

Building Blocks ◽

Careful Consideration ◽

Breeding Lines ◽

Biological Phenomena

Plants are fundamental to life, being the basis of our food production and an essential part of the global ecosystem on which life on earth depends. Plant genetic resources include primitive forms of cultivated plant species and landraces, modern cultivars, breeding lines and genetic stocks, weedy types and related wild species, which provide the building blocks that, allow classical plant breeders and biotechnologists to develop new commercial varieties and other biological products. Detection and analysis of genetic variation can help us to understand the molecular basis of various biological phenomena in plants. Molecular markers for the detection and exploitation of DNA polymorphism is one of the most significant developments in the field of molecular genetics. The presence of various types of molecular markers, and differences in their principles, methodologies, and applications require careful consideration in choosing one or more of such methods. This article describes the advances of molecular marker in present, introduces the molecular basis in development of plant genetic resources and perspectives the important role of molecular marker in development of plant genetic resources in the future.

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