FIBRONECTIN CONTENTS AND LEVELS IN BLOOD COMPONENTS DURING STORAGE

Mapping Intimacies ◽

10.1055/s-0038-1644155 ◽

1987 ◽

Author(s):

N Müller ◽

U Velten

Keyword(s):

Critically Ill ◽

Critically Ill Patients ◽

Storage Period ◽

Functional Tests ◽

Platelet Concentrates ◽

Platelet Storage ◽

Turbidimetric Assay ◽

Effective Use ◽

Plastic Containers ◽

Packed Rbcs

Fibronectin has been proposed to have an antithrombotic effect, protecting against platelet and fibrinogen consumption after injury. For the supply of platelets the possibility of extending platelet storage is important forthe management of platelet logistics. This study was designed to determine the effect of storage on the contents and levels of fibro-nectin (FN) in whole blood and components suchas packed RBCs, PRPs and platelet concentrates (PC) in two different plastics. For care of critically ill patients the FN present in components often used in large amounts could supplement the use of purified FN as a source of this opsonic protein. FN protein was assayed using an electroimmunoassay as well as a turbidimetric assay for quantitative determination at 2 day intervals during storage of CPDA-1 stabilized red cells at 4° C for 35 days and daily during end-over-end rotational storage of platelets at 22° C in conventional plastic containers (I) and trimellitate plasticised polyvinylchlo-ride bags (II) (F-763 Biotest). Moreover platelet functional tests, fibrinogen, F XIII and F VIII-complex were tested. FN levels in red cell componentsgradually decreased during storage until to 40% of the initial levels. Platelets maintained a concentrationof 404 ±70 ug/dl (I) and 378±66 ug/dl(II). There were no significant differences between the values determined in the two differentbags over the 8-days storage period. This study demonstrate the stabilityof FN protein during storage and formore effective use of limited donor resources the FN content of each of these products should be considered when determing the dose of FN for replacement therapy in critically ill patients with FN depletion following trauma and surgery.

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Platelet storage at 22 degrees C: role of gas transport across plastic containers in maintenance of viability

Blood ◽

10.1182/blood.v46.2.209.209 ◽

1975 ◽

Vol 46 (2) ◽

pp. 209-218 ◽

Cited By ~ 146

Author(s):

S Murphy ◽

FH Gardner

Keyword(s):

Platelet Count ◽

Lactate Concentration ◽

Platelet Concentrates ◽

Pasteur Effect ◽

Platelet Storage ◽

Low Levels ◽

Microscopic Morphology ◽

Plastic Containers

Abstract Containers constructed of polyvinylchloride (PVC) are used for the storage of platelet concentrates (PC) for transfusion, At 22 degrees C, pH often falls to such low levels (pH is less that 6.0) that viability is lost. Far lesser degrees of pH fall are observed in bags constructed of polyethylene (PE). In this study, pH, PO2, PCO2, platelet count, lactate concentration, microscopic morphology, and viability after 51- chromium labeling were evaluated during storage at 22 degrees C under a variety of circumstances. The results indicate that (1) pH falls because of the generation of lactic acid by platelet glycolysis and, under some circumstances, the retention of CO2. (2) Rate of pH fall is, therefore, roughly proportional to the platelet count. (3) PE is more permeable to gases, thereby allowing CO2 escape from and easier O2 entry into the stored PC; the higher O2 tensions suppress glycolysis by the Pasteur effect. (4) Adequate agitation and container size are critical if the beneficial effect of PE is to be obtained. (5) In general, platelets stored in PE containers have excellent viability in vivo although CO2 escape can result in elevations in pH which are deleterious. (6) Storage in a 10% CO2 atmosphere prevents these deletrrious pH elevations without otherwise impairing platelet viability; (7) Results similar to those achieved with PE can be achieved with PVC if this material is made thinner to allow easier penetration of gases.

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Atypical Bacterial Growth within Units of Platelets Challenges Transfusion Medicine Dogma

Journal of Clinical Microbiology ◽

10.1128/jcm.01363-18 ◽

2018 ◽

Vol 56 (12) ◽

Cited By ~ 3

Author(s):

Eric A. Gehrie

Keyword(s):

Bacterial Contamination ◽

Current Issue ◽

Safety Concern ◽

Positive Culture ◽

Storage Period ◽

Transfusion Medicine ◽

Platelet Concentrates ◽

Content Type ◽

Platelet Storage ◽

Positive Culture Result

ABSTRACT Although transfusion-transmitted bacterial infection is relatively rare, mitigation of bacterial contamination of platelet units is arguably the top current transfusion-related safety concern. Several different technologies have been employed to detect or neutralize bacteria in platelet concentrates. However, studies of the efficacy of these systems have been hampered by problematic definitions of what represents a “true-positive” versus a “false-positive” culture result. In the current issue of the Journal of Clinical Microbiology (M. Cloutier, M.-È. Nolin, H. Daoud, A. Jacques, M. J. de Grandmont, É Ducas, G. Delage, and L. Thibault, J Clin Microbiol 56:e01105-18, 2018, https://doi.org/10.1128/JCM.01105-18), it was demonstrated that the growth of Bordetella holmesii is inhibited by the platelet storage environment, which may explain why the results of initial positive platelet cultures are not always confirmed by subsequent cultures later during the storage period. This important finding is at odds with the generally held belief within the field of transfusion medicine that initially positive platelet cultures that are not confirmed on repeat testing are instrumentation-based false positives. The clinical risk profile of organisms demonstrating storage-related low viability is worthy of further study.

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Duration of platelet storage and outcomes of critically ill patients

Transfusion ◽

10.1111/trf.14056 ◽

2017 ◽

Vol 57 (3) ◽

pp. 599-605 ◽

Cited By ~ 5

Author(s):

Andrew Flint ◽

Cécile Aubron ◽

Michael Bailey ◽

Rinaldo Bellomo ◽

David Pilcher ◽

...

Keyword(s):

Critically Ill ◽

Critically Ill Patients ◽

Platelet Storage

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Effects of use of riboflavin and ultraviolet light for pathogen inactivation on quality of platelet concentrates

Vojnosanitetski pregled ◽

10.2298/vsp1106489s ◽

2011 ◽

Vol 68 (6) ◽

pp. 489-494 ◽

Cited By ~ 2

Author(s):

Zoran Stanojkovic ◽

Ana Antic ◽

Miodrag Stojanovic

Keyword(s):

Uv Light ◽

Storage Period ◽

Buffy Coat ◽

Blood Products ◽

Pathogen Inactivation ◽

Platelet Concentrates ◽

Platelet Storage ◽

Control Units ◽

The Moment ◽

Uv Rays

Background/Aim. Pathogen inactivation in blood and blood products is one of the major means to achieve a zero risk blood supply and improve transfusion safety. Riboflavin (vitamin B2) activated by ultraviolet (UV) light, produces active oxygen which damages cell membrane and prevents replication of the carrier of diseases (viruses, bacteria, protozoa) in all blood products. The aim of this study was to establish the influence of the process of pathogens photoinactivation using riboflavin and UV rays on the biochemical and functional characteristics of platelet concentrates prepared from ?buffy coat?. Methods. The examination included 80 platelet concentrates prepared from ?buffy coat?, which was separated from whole blood donated by voluntary blood donors around 6 hours from the moment of collection. Concentrates were pooled, filtered and separated unton two groups: one consisted of 10 control units and the other of 10 examined units (pooled platelet concentrates). Examined units of the platelets were treated by riboflavin (35 mL) and UV rays (6.24 J/mL, 265-370 nm) on Mirasol aparature (Caridian BCT Biotechnologies, USA) in approximate duration of 6 min. A total of 35 mL of saline solution was added to the control units. The samples for examining were taken from the control and examined units initially (K0, I0), after the addition of saline (K1) and riboflavin (I1), after illumination (I2), first day of storage (K3, I3) and the fifth day of storage (K4, I4). The following parameters were measured: platelet count and platelet yield, residual erythrocyte and leukocyte count, pH, pO2, pCO2 and bacterial contamination. Results. All the measured parameters showed a statistically significant decrease comparing to K0 and I0; all the results of the first day of platelet storage showed statistically significant decrease comparing to K1 and I1, and all the results of the fifth day of platelet storage (K4, I4) showed a statistically significant decrease comparing to K1 and K3 and to I1 and I3. There was no the mentioned difference in the measured parameters between K4 and I4 (the end of storage - the fifth day). All the platelet units were sterile till the seventh day, when the investigation ended. Conclusion. Platelet concentrates inactivated by riboflavin and UV rays (Mirasol PRT sistem, Caridian BCT, USA) keep all the characteristics assessed by the Guide to the preparation, use and quality assurance of blood components (Council of Europe), during the whole storage period (five days). The obtained data were correlated with existing up to date literature and demonstrated that Mirasol treated platelets were safe and could be incorporated effectively in the routine blood bank and transfusion setting.

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Platelet storage at 22 degrees C: role of gas transport across plastic containers in maintenance of viability

Blood ◽

10.1182/blood.v46.2.209.bloodjournal462209 ◽

1975 ◽

Vol 46 (2) ◽

pp. 209-218 ◽

Cited By ~ 3

Author(s):

S Murphy ◽

FH Gardner

Keyword(s):

Platelet Count ◽

Lactate Concentration ◽

Platelet Concentrates ◽

Pasteur Effect ◽

Platelet Storage ◽

Low Levels ◽

Microscopic Morphology ◽

Plastic Containers

Containers constructed of polyvinylchloride (PVC) are used for the storage of platelet concentrates (PC) for transfusion, At 22 degrees C, pH often falls to such low levels (pH is less that 6.0) that viability is lost. Far lesser degrees of pH fall are observed in bags constructed of polyethylene (PE). In this study, pH, PO2, PCO2, platelet count, lactate concentration, microscopic morphology, and viability after 51- chromium labeling were evaluated during storage at 22 degrees C under a variety of circumstances. The results indicate that (1) pH falls because of the generation of lactic acid by platelet glycolysis and, under some circumstances, the retention of CO2. (2) Rate of pH fall is, therefore, roughly proportional to the platelet count. (3) PE is more permeable to gases, thereby allowing CO2 escape from and easier O2 entry into the stored PC; the higher O2 tensions suppress glycolysis by the Pasteur effect. (4) Adequate agitation and container size are critical if the beneficial effect of PE is to be obtained. (5) In general, platelets stored in PE containers have excellent viability in vivo although CO2 escape can result in elevations in pH which are deleterious. (6) Storage in a 10% CO2 atmosphere prevents these deletrrious pH elevations without otherwise impairing platelet viability; (7) Results similar to those achieved with PE can be achieved with PVC if this material is made thinner to allow easier penetration of gases.

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