Anatomopathological Assessment of the Diaphragm in Formalin-Fixed, Paraffin-Embedded Sections

Introduction The analysis of frozen muscle biopsies has become a routine method in the evaluation of muscle structure in health and disease. However, the technique for frozen muscle specimens is not widely available in countries with limited medical facilities. The present study aimed to elucidate a reproducible formalin-fixed and paraffin-embedded (FFPE) method for this type of analysis in postmortem muscles. Methods Diaphragm muscle was obtained within 1 hour of sudden death. Diaphragm strips were washed in saline solution, fixed in 10% formalin, frozen at 4°C in a refrigerator, and stored for 24 hours. Then, the tissue samples were processed into paraffin-embedded blocks. Transversal sections were cut from each paraffin block and stained with hematoxylin and eosin, Picrosirius red, Verhoeff-Van Gieson, and Congo red for the qualitative analysis. Results Our analysis indicated a well-preserved muscle. Conclusion In summary, we demonstrate a simple technique for a reproducible FFPE method in postmortem muscle tissues.

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A Method to Reuse Archived H&E Stained Histology Slides for a Multiplex Protein Biomarker Analysis

Methods and Protocols ◽

10.3390/mps2040086 ◽

2019 ◽

Vol 2 (4) ◽

pp. 86

Author(s):

James P. Hinton ◽

Katerina Dvorak ◽

Esteban Roberts ◽

Wendy J. French ◽

Jon C. Grubbs ◽

...

Keyword(s):

Human Tumor ◽

Clinical Value ◽

Tissue Samples ◽

Analysis Techniques ◽

Formalin Fixed Paraffin ◽

Biomarker Analysis ◽

Formalin Fixed Paraffin Embedded ◽

Hematoxylin And Eosin ◽

Image Analysis Techniques ◽

Formalin Fixed

Archived Hematoxylin and Eosin (H&E) stained pathology slides are routinely stored to index formalin-fixed paraffin-embedded (FFPE) sample tissue blocks. FFPE blocks are clinically annotated human tumor specimens that can be valuable in studies decades after the tissue is collected. If stored properly, they have the potential to yield a valuable number of serial sectioned slides for diagnostic or research purposes. However, some retrospective studies are limited in scope because the tissue samples have been depleted or not enough material is available in stored blocks for serial sections. The goal of these studies was to determine if archived H&E-stained slides can be directly reutilized by optimizing methods to de-stain and then re-stain the H&E stained slides to allow the detection of several biomarkers of interest using a conjugated antibody with chromogen multiplex immunohistochemistry procedure. This simple but innovative procedure, combined with image analysis techniques, demonstrates the ability to perform precise detection of relevant markers correlated to disease progression in initially identified tumor regions in tissue. This may add clinical value in retaining H&E slides for further use.

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Relationship between Angiogenic Squamous Dysplasia and Bronchogenic Carcinoma in Patients Undergoing White Light Bronchoscopy

Canadian Respiratory Journal ◽

10.1155/2012/343954 ◽

2012 ◽

Vol 19 (3) ◽

pp. 201-206 ◽

Cited By ~ 1

Author(s):

Shirin Karimi ◽

Forouzan Mohammadi ◽

Kian Khodadad ◽

Makan Sadr ◽

Leila Seyfollahi ◽

...

Keyword(s):

White Light ◽

Bronchogenic Carcinoma ◽

Control Group ◽

Screening Programs ◽

Formalin Fixed Paraffin ◽

Neoplastic Lesions ◽

Formalin Fixed Paraffin Embedded ◽

Embedded Blocks ◽

Hematoxylin And Eosin ◽

Formalin Fixed

OBJECTIVE: To better understand the characteristic morphology of angiogenic squamous dysplasia (ASD) and its association with different types of common bronchogenic carcinomas using routine white light bronchoscopy.METHODS: Using a case-control design, 186 formalin-fixed paraffin-embedded blocks of bronchial tissue (136 cases, 50 controls) obtained from patients who underwent routine nonfluorescence bronchoscopy between 2004 and 2005 were studied.RESULTS: ASD occurred at a higher frequency in patients with neoplastic lesions compared with those without neoplastic lesions (28 of 136 versus one of 50). ASD was also more prevalent in patients with squamous cell carcinoma compared with other neoplasms. Seventy six per cent of the ASD patients (22 of 29) smoked cigarettes. The morphology of ASD on hematoxylin and eosin- and CD31-stained sections was characterized by prominent microvasculature and capillary projections closely juxtaposed to variable degrees of dysplasia in all of the bronchogenic carcinoma specimens, and to metaplasia in one case in the control group.CONCLUSION: ASD is a unique morphological entity that should be considered by pathologists even on bronchoscopic biopsies from patients who undergo white light bronchoscopy. The presence of ASD may represent a risk biomarker of bronchogenic carcinoma in screening programs and in chemoprevention of lung cancer.

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New Information in Old Formalin-Fixed, Paraffin-Embedded Blocks: Does the Paraffin Block Lose its Value in the Drawer Over Years?

American Journal of Clinical Pathology ◽

10.1093/ajcp/138.suppl1.090 ◽

2012 ◽

Vol 138 (suppl 1) ◽

pp. A095-A095

Author(s):

Zachery G. vonMenchhofen ◽

Diane McGarvey ◽

Walter Miller ◽

Susan Dinella ◽

Andrea Blatt ◽

...

Keyword(s):

New Information ◽

Formalin Fixed Paraffin ◽

Paraffin Block ◽

Formalin Fixed Paraffin Embedded ◽

Embedded Blocks ◽

Formalin Fixed

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HTAPP_Sectioning formalin fixed paraffin embedded (FFPE) tissue samples for hematoxylin and eosin (H&E) stained slides v1 (protocols.io.bu3inyke)

protocols.io ◽

10.17504/protocols.io.bu3inyke ◽

2021 ◽

Author(s):

Ana Lako ◽

Kathleen L ◽

Scott Rodig

Keyword(s):

Ffpe Tissue ◽

Tissue Samples ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Hematoxylin And Eosin ◽

Formalin Fixed

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The Dutch National TissueArchive Portal enables efficient, consistent, and transparent procurement of diagnostic tissue samples for scientific use

Cell and Tissue Banking ◽

10.1007/s10561-021-09949-1 ◽

2021 ◽

Author(s):

Robin Verjans ◽

Annette H. Bruggink ◽

Robby Kibbelaar ◽

Jos Bart ◽

Aletta Debernardi ◽

...

Keyword(s):

The Netherlands ◽

Tissue Sample ◽

Ffpe Tissue ◽

Tissue Samples ◽

Internet Portal ◽

Formalin Fixed Paraffin ◽

Practical Support ◽

Formalin Fixed Paraffin Embedded ◽

The Rich ◽

Formalin Fixed

AbstractBiobanks play a crucial role in enabling biomedical research by facilitating scientific use of valuable human biomaterials. The PALGA foundation—a nationwide network and registry of histo- and cytopathology in the Netherlands—was established to promote the provision of data within and between pathology departments, and to make the resulting knowledge available for healthcare. Apart from the pathology data, we aimed to utilize PALGA’s nationwide network to find and access the rich wealth of Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples for scientific use. We implemented the Dutch National TissueArchive Portal (DNTP) to utilize PALGA’s nationwide network for requesting FFPE tissue samples. The DNTP consists of (1) a centrally organized internet portal to improve the assessing, processing, harmonization, and monitoring of the procurement process, while (2) dedicated HUB-employees provide practical support at peripheral pathology departments. Since incorporation of the DNTP, both the number of filed requests for FFPE tissue samples and the amount of HUB-mediated support increased 55 and 29% respectively. In line, the sample procurement duration time decreased significantly (− 47%). These findings indicate that implementation of the DNTP improved the frequency, efficiency, and transparency of FFPE tissue sample procurement for research in the Netherlands. To conclude, the need for biological resources is growing persistently to enable precision medicine. Here, we access PALGA’s national, pathology network by implementation of the DNTP to allow for efficient, consistent, and transparent exchange of FFPE tissue samples for research across the Netherlands.

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Detection of clonality in follicular lymphoma using formalin-fixed, paraffin-embedded tissue samples and BIOMED-2 immunoglobulin primers

Journal of Clinical Pathology ◽

10.1136/jcp.2010.081109 ◽

2010 ◽

Vol 64 (1) ◽

pp. 37-41 ◽

Cited By ~ 28

Author(s):

E. Berget ◽

L. Helgeland ◽

A. Molven ◽

O. K. Vintermyr

Keyword(s):

Follicular Lymphoma ◽

Tissue Samples ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed

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Microsatellite instability evaluation: which test to use for endometrial cancer?

Journal of Clinical Pathology ◽

10.1136/jclinpath-2021-207723 ◽

2021 ◽

pp. jclinpath-2021-207723

Author(s):

Paola Rafaniello-Raviele ◽

Ilaria Betella ◽

Alessandra Rappa ◽

Davide Vacirca ◽

Gianluca Tolva ◽

...

Keyword(s):

Endometrial Cancer ◽

Microsatellite Instability ◽

Automated System ◽

Tissue Samples ◽

Valuable Alternative ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Formalin Fixed ◽

Mmr Proteins

AimsAnalysis of microsatellite instability (MSI) is strongly recommended in endometrial cancer (EC) and colorectal cancer to screen for Lynch syndrome, to predict prognosis and to determine optimal treatment and follow-up. In a large monoinstitutional series of ECs, we evaluated the reliability and accuracy of Idylla assay, a rapid, fully automated system to detect MSI, and we compared its performance with two routine reference methods.MethodsWe evaluated MSI status in 174 formalin-fixed, paraffin-embedded EC tissue samples using immunohistochemistry (IHC) for mismatch repair (MMR) proteins and Idylla assay. Samples with discordant or equivocal results were analysed with a third technique, the Promega MSI kit.ResultsIdylla MSI assay and IHC were highly concordant (overall agreement: 154/170=90.59%, 95% CI 85.26% to 94.12%). However, in four samples, MMR-IHC staining was equivocal; moreover, 16 cases showed discordant results, that is, MMR deficient using IHC and microsatellite stable using Idylla. These 20 samples were reanalysed using the MSI-Promega kit, which showed the same results of Idylla assay in 18/20 cases (overall agreement: 90%, 95% CI 69.90% to 97.21%).ConclusionsOur results suggest that IHC is an efficient method to determine MMR status in ECs. However, the Idylla MSI assay is a rapid and reliable tool to define MSI status, and it could represent a valuable alternative to conventional MSI-PCR methods.

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Canine Hepatitis Associated with Intrahepatic Bacteria in Three Dogs

Journal of the American Animal Hospital Association ◽

10.5326/jaaha-ms-6492 ◽

2018 ◽

Vol 54 (1) ◽

pp. 65-70 ◽

Cited By ~ 3

Author(s):

Joon Im ◽

Derek P. Burney ◽

Sean P. McDonough ◽

Brigid Nicholson ◽

Adam Eatroff ◽

...

Keyword(s):

In Situ Hybridization ◽

Case Report ◽

Fluorescence In Situ Hybridization ◽

Antimicrobial Therapy ◽

Granulomatous Inflammation ◽

Formalin Fixed Paraffin ◽

Formalin Fixed Paraffin Embedded ◽

Hematoxylin And Eosin ◽

Formalin Fixed

ABSTRACT This case report describes the detection of intrahepatic bacteria in formalin-fixed paraffin-embedded histopathological sections from three dogs with neutrophilic, pyogranulomatous, or lymphoplasmacytic hepatitis and cholangiohepatitis. In each of these cases, eubacterial fluorescence in situ hybridization enabled colocalization of intrahepatic bacteria with neutrophilic and granulomatous inflammation in samples that were negative for bacteria when evaluated by routine hematoxylin and eosin histopathology augmented with histochemical stains. Positive responses to antimicrobial therapy were observed in of 2 out of 2 patients that were treated with antimicrobials. These findings suggest that eubacterial fluorescence in situ hybridization analysis of formalin-fixed paraffin-embedded histopathological sections is more sensitive than conventional histochemical stains for the diagnosis of bacteria-associated canine hepatitis.

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