scholarly journals New Information in Old Formalin-Fixed, Paraffin-Embedded Blocks: Does the Paraffin Block Lose its Value in the Drawer Over Years?

2012 ◽  
Vol 138 (suppl 1) ◽  
pp. A095-A095
Author(s):  
Zachery G. vonMenchhofen ◽  
Diane McGarvey ◽  
Walter Miller ◽  
Susan Dinella ◽  
Andrea Blatt ◽  
...  
Keyword(s):  
New Information ◽  
Formalin Fixed Paraffin ◽  
Paraffin Block ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
Formalin Fixed

scholarly journals Anatomopathological Assessment of the Diaphragm in Formalin-Fixed, Paraffin-Embedded Sections

2018 ◽  
Vol 35 (03) ◽  
pp. 173-176 ◽  
Author(s):  
Ricardo Nucci ◽  
Wilson Jacob-Filho ◽  
Alexandre Busse ◽  
Laura Maifrino ◽  
Romeu de Souza
Keyword(s):  
Diaphragm Muscle ◽  
Tissue Samples ◽  
Muscle Tissues ◽  
Formalin Fixed Paraffin ◽  
Paraffin Block ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
Health And Disease ◽  
Hematoxylin And Eosin ◽  
Formalin Fixed

Introduction The analysis of frozen muscle biopsies has become a routine method in the evaluation of muscle structure in health and disease. However, the technique for frozen muscle specimens is not widely available in countries with limited medical facilities. The present study aimed to elucidate a reproducible formalin-fixed and paraffin-embedded (FFPE) method for this type of analysis in postmortem muscles. Methods Diaphragm muscle was obtained within 1 hour of sudden death. Diaphragm strips were washed in saline solution, fixed in 10% formalin, frozen at 4°C in a refrigerator, and stored for 24 hours. Then, the tissue samples were processed into paraffin-embedded blocks. Transversal sections were cut from each paraffin block and stained with hematoxylin and eosin, Picrosirius red, Verhoeff-Van Gieson, and Congo red for the qualitative analysis. Results Our analysis indicated a well-preserved muscle. Conclusion In summary, we demonstrate a simple technique for a reproducible FFPE method in postmortem muscle tissues.

scholarly journals A correlative chemical and histochemical study of the O-acetylated sialic acids of human colonic epithelial glycoproteins in formalin fixed paraffin embedded tissues.

1978 ◽  
Vol 26 (12) ◽  
pp. 1033-1041 ◽  
Author(s):  
P E Reid ◽  
C F Culling ◽  
W L Dunn ◽  
M G Clay ◽  
C W Ramey
Keyword(s):  
Epithelial Cells ◽  
Histochemical Study ◽  
Sialic Acids ◽  
Side Chain ◽  
Isolation And Identification ◽  
New Information ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Human Large Intestine ◽  
Formalin Fixed

Procedures are described for the isolation and identification of epithelial glycoproteins from formalin fixed, paraffin embedded specimens of human large intestine. The side chain O-acetylation patterns of the sialic acids of these glycoproteins were surprisingly similar to those of purified glycoproteins prepared from epithelial cells obtained from the same tissue before fixation. These results were consistent with those obtained by histochemical procedures performed on representative sections taken from the same tissue blocks. The methodology described permits a direct correlation of chemical and histochemical results obtained from the study of colonic epithelial glycoproteins of both normal and diseased tissues. It eliminates some of the difficulties associated with interpretation of the results by either discipline and may provide new information which would be unavailable by either chemistry or histochemistry alone.

scholarly journals Genetic Diversity of Hepatic/Non-Hepatic Cystic Echinococ-cosis in Baqiyatallah Hospital, Tehran, Iran

2020 ◽  
Author(s):  
Hamidreza NEYSI ◽  
Tahereh MOHAMMADZADEH ◽  
Seyed Mahmoud SADJJADI ◽  
Jamal AKHAVANMOGHADDAM ◽  
Alireza SHAMSAEI
Keyword(s):  
Sensu Stricto ◽  
Sequence Alignments ◽  
Oxidase Gene ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
Related Genotype ◽  
Polymerase Chain ◽  
Organ Tropism ◽  
Formalin Fixed

Background: Cystic echinococcosis (CE) is a worldwide zoonotic helminthic disease caused by the larval stage of Echinococcus granulosus. The infection is particularly important in terms of economic and medico-veterinary aspects in endemic areas including Iran. Considering the possibility of organ-tropism in E. granulosus strains, the present study was aimed to identify the genotypes of E. granulosus in different organs involved in patients, undergone surgery in Baqiyatallah Hospital, Tehran, Iran from 2005-2015. Methods: Overall, 29 formalin-fixed paraffin-embedded tissues (FFPT) from patients with histologically confirmed CE including liver (N: 14) lungs (N: 6) abdomen (N: 2), pancreas (N: 2) and each of spleen, gallbladder and, muscles (N: 1) plus unknown organs (N: 2) were used and genetically characterized using polymerase chain reaction, followed by partial sequencing of mitochondrial cytochrome c oxidase gene subunit 1(cox1) and analyzed. Results: Nineteen out of 29 isolates including liver (N: 6) lungs (N: 4) abdomen (N: 2), pancreas (N: 2) and each of spleen, gallbladder, and muscle (N: 1), unknown organs (N: 2) obtained from paraffin-embedded blocks of human CE created an acceptable sequence in two directions. All 19 isolates regardless of the organ involved were recognized as E. granulosus sensu stricto (G1). Conclusion: The sequence alignments of the isolates displayed two profiles. All sequenced samples showed E. granulosus sensu stricto (G1) with no organ-related genotype.

scholarly journals Ovarian Cancer Translational Activity of the Multicenter Italian Trial in Ovarian Cancer (MITO) Group: Lessons Learned in 10 Years of Experience

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 903
Author(s):  
Daniela Califano ◽  
Daniela Russo ◽  
Giosuè Scognamiglio ◽  
Nunzia Simona Losito ◽  
Anna Spina ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Clinical Trials ◽  
Clinical Data ◽  
Gynecologic Cancer ◽  
Years Of Experience ◽  
Web Based ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
Formalin Fixed

Ovarian cancer is the most lethal gynecological cancer, and despite years of research, with the exception of a BRCA mutation driving the use of PARP inhibitors, no new prognostic/predictive biomarkers are clinically available. Improvement in biomarker selection and validation may derive from the systematic inclusion of translational analyses into the design of clinical trials. In the era of personalized medicine, the prospective centralized collection of high-quality biological material, expert pathological revision, and association to well-controlled clinical data are important or even essential added values to clinical trials. Here, we present the academic experience of the MITO (Multicenter Italian Trial in Ovarian Cancer) group, including gynecologists, pathologists, oncologists, biostatisticians, and translational researchers, whose effort is dedicated to the care and basic/translational research of gynecologic cancer. In our ten years of experience, we have been able to collect and process, for translational analyses, formalin-fixed, paraffin-embedded blocks from more than one thousand ovarian cancer patients. Standard operating procedures for collection, shipping, and processing were developed and made available to MITO researchers through the coordinating center’s web-based platform. Clinical data were collected through dedicated electronic case report forms hosted in a web-based electronic platform and stored in a central database at the trial’s coordinating center, which performed all the analyses related to the proposed translational researches. During this time, we improved our strategies of block management from retrospective to prospective collection, up to the design of a prospective collection with a quality check for sample eligibility before patients’ accrual. The final aim of our work is to share our experience by suggesting a guideline for the process of centralized collection, revision processing, and storing of formalin-fixed, paraffin-embedded blocks for translational purposes.

scholarly journals Distribution of Tight Junction Proteins in Adult Human Salivary Glands

2008 ◽  
Vol 56 (12) ◽  
pp. 1093-1098 ◽  
Author(s):  
Ola M. Maria ◽  
Jung-Wan Martin Kim ◽  
Jonathan A. Gerstenhaber ◽  
Bruce J. Baum ◽  
Simon D. Tran
Keyword(s):  
Endothelial Cells ◽  
Salivary Gland ◽  
Salivary Glands ◽  
Adult Human ◽  
New Information ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Molecular Components ◽  
Formalin Fixed ◽  
Junction Proteins

Tight junctions (TJs) are an essential structure of fluid-secreting cells, such as those in salivary glands. Three major families of integral membrane proteins have been identified as components of the TJ: claudins, occludin, and junctional adhesion molecules (JAMs), plus the cytosolic protein zonula occludens (ZO). We have been working to develop an orally implantable artificial salivary gland that would be suitable for treating patients lacking salivary parenchymal tissue. To date, little is known about the distribution of TJ proteins in adult human salivary cells and thus what key molecular components might be desirable for the cellular component of an artificial salivary gland device. Therefore, the aim of this study was to determine the distribution of TJ proteins in human salivary glands. Salivary gland samples were obtained from 10 patients. Frozen and formalin-fixed paraffin-embedded sections were stained using IHC methods. Claudin-1 was expressed in ductal, endothelial, and ∼25% of serous cells. Claudins-2, −3, and −4 and JAM-A were expressed in both ductal and acinar cells, whereas claudin-5 was expressed only in endothelial cells. Occludin and ZO-1 were expressed in acinar, ductal, and endothelial cells. These results provide new information on TJ proteins in two major human salivary glands and should serve as a reference for future studies to assess the presence of appropriate TJ proteins in a tissue-engineered human salivary gland.

scholarly journals Immunohistochemical expression of Cyclin D1 among Sudanese patients diagnosed with benign and malignant prostatic lesions

2019 ◽  
Author(s):  
Eiman Siddig Ahmed ◽  
Lubna S. Elnour ◽  
Rowa Hassan ◽  
Emmanuel Edwar Siddig ◽  
Mintu Elsa Chacko ◽  
...  
Keyword(s):  
Family History ◽  
Cyclin D1 ◽  
P Value ◽  
Low Grade ◽  
High Grade ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
History Of ◽  
Formalin Fixed

Abstract Objectives: Prostate cancer (PC) is common cancer worldwide. Several markers have been developed to differentiate between benign prostatic hyperplasia (BPH) from PC. A descriptive retrospective hospital-based study aimed at determining the expression of Cyclin D1 in BPH and PC. the study took place at different histopathology laboratories in Khartoum state, Sudan, from December 2016 to January 2019. Formalin-fixed paraffin-embedded blocks were sectioned and fixed in 3-aminopropyltriethoxysilane coated slides incubated into primary antibody for Cyclin D1. The assessment of immunoreactivity of Cyclin D1 of each section was done using the Gleason scoring system. Results: A total of 153 males’ prostate sections included in this study, of them, 120 (78.4%) were PC, and 33 (21.6%) were BPH. Their age ranged from 45 to 88 years, mean age was 66.19 ± 8.599. 142 (92.8%) did not have a family history of PC, while 11 (7.2%) patients reported having a family history. The Gleason scoring showed a total of 81 (52.9%) patients with high-grade and 39 (25.5%) with low-grade. 118 (97.5%) patients had PC showed positive results for Cyclin D1, while BPH was 3 (2.5%). P value < 0.001. Cyclin D1 staining was associated with high-grade Gleason score and perineural invasion, P value 0.001.

scholarly journals Tissue Digestion for Ferruginous Bodies for Light Microscopy

1998 ◽  
Vol 6 (1) ◽  
pp. 8-11
Author(s):  
Margaret Gondo
Keyword(s):  
Tissue Sample ◽  
Lung Parenchyma ◽  
Asbestos Fibers ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
The Individual ◽  
Ferruginous Bodies ◽  
Formalin Fixed ◽  
Tissue Digestion

Asbestos fibers are commonly called ferruginous bodies due to the iron content of the fibers. After they are inhaled, the asbestos fibers become coated with glycoprotein and hemosiderin, deposited by macrophages.This procedure may be used on either formalin fixed, paraffin embedded blocks or formalin fixed wet tissue. Because this procedure will result in complete destruction of the tissue sample used, it is very important to obtain written approval documenting this fact from the individual requesting this procedure, if the procedure is being done for someone else. The tissue should be from the lung parenchyma. Also, it is a good idea to use sterile, disposable tubes throughout this procedure to limit the possibility of contamination.

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