A Radioreceptor Assay for Insulin: Direct Measurement of Human Serum Insulin

Abstract We applied a "sandwich" method, with use of beads coated with anti-insulin serum and of peroxidase-labeled anti-insulin serum, to an enzyme immunoassay of insulin in human serum. 5-Aminosalicylic acid was used as the substrate for the enzymic reaction. As little as 5 milli-int. units of insulin per liter of serum insulin was detectable. Reproducibility was satisfactory, but extraordinarily high concentrations of proinsulin and of hydrogen donors such as reduced glutathione affect results of the assay. Values determined by our enzyme immunoassay and by double-antibody radioimmunoassay correlated highly (r = 0.938, p less than 0.001, n = 216). We recommend this method for use in the clinical laboratory.

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The measurement of free nitrendipine in human serum by an equilibrium dialysis - radioreceptor assay

Life Sciences ◽

10.1016/0024-3205(83)90690-2 ◽

1983 ◽

Vol 33 (18) ◽

pp. 1821-1829 ◽

Cited By ~ 11

Author(s):

Howard R. Lee ◽

William R. Roeske ◽

Henry I. Yamamura

Keyword(s):

Human Serum ◽

Equilibrium Dialysis ◽

Radioreceptor Assay

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Analysis of UK Sera for Aflatoxin by Enzyme-linked Immunosorbent Assay

Human Toxicology ◽

10.1177/096032718800700410 ◽

1988 ◽

Vol 7 (4) ◽

pp. 353-356 ◽

Cited By ~ 18

Author(s):

A.P. Wilkinson ◽

D.W. Denning ◽

M.R.A. Morgan

Keyword(s):

Human Serum ◽

Direct Measurement ◽

Immunosorbent Assay ◽

Human Exposure ◽

Blood Donors ◽

Enzyme Linked Immunosorbent Assay ◽

Fungal Metabolites ◽

Serum Samples ◽

Imported Food ◽

The Uk

1 Aflatoxins are toxic, carcinogenic secondary fungal metabolites produced by certain moulds that commonly infest foods. Measurement of aflatoxins in human serum would give a direct measurement of exposure. 2 Twenty-seven serum samples from UK blood donors were found to contain aflatoxin levels not greater than 64 pmol/1 (20 pg/ml) by an enzyme-linked immunosorbent assay. 3 These findings may indicate that present UK guideline tolerances for aflatoxin in imported food are effective in limiting human exposure to toxic aflatoxins in the UK diet, though further work would be needed to confirm this. In particular, sub-populations suspected of being at higher risk may need special considerations.

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A novel method for direct measurement of complement convertases activity in human serum

Clinical & Experimental Immunology ◽

10.1111/cei.12388 ◽

2014 ◽

Vol 178 (1) ◽

pp. 142-153 ◽

Cited By ~ 26

Author(s):

A. M. Blom ◽

E. B. Volokhina ◽

V. Fransson ◽

P. Strömberg ◽

L. Berghard ◽

...

Keyword(s):

Human Serum ◽

Direct Measurement ◽

Novel Method

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The nature of human serum insulin-like activity (ILA): characterization of ILA in serum and serum fractions obtained by acid-ethanol extraction and adsorption chromatography

Journal of Clinical Investigation ◽

10.1172/jci105726 ◽

1968 ◽

Vol 47 (2) ◽

pp. 301-320 ◽

Cited By ~ 20

Author(s):

Philip L. Poffenbarger ◽

John W. Ensinck ◽

Dieter K. Hepp ◽

Robert H. Williams

Keyword(s):

Human Serum ◽

Serum Insulin ◽

Adsorption Chromatography ◽

Ethanol Extraction ◽

Serum Fractions ◽

Insulin Like Activity

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Radioreceptor assay for 1.ALPHA.,24(R)25-trihydroxyvitamin D3 in human serum.

Journal of Nutritional Science and Vitaminology ◽

10.3177/jnsv.27.71 ◽

1981 ◽

Vol 27 (1) ◽

pp. 71-75 ◽

Cited By ~ 9

Author(s):

Seiichi ISHIZUKA ◽

Tatsuyuki NARUCHI ◽

Yoshinobu HASHIMOTO ◽

Hajime ORIMO

Keyword(s):

Human Serum ◽

Radioreceptor Assay

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Double-Antibody Radioimmunoassay of Serum Insulin: Effect of Use of Hormone-Depleted Human Serum

Clinical Chemistry ◽

10.1093/clinchem/21.10.1520 ◽

1975 ◽

Vol 21 (10) ◽

pp. 1520-1522 ◽

Cited By ~ 6

Author(s):

Cynthia K Silbert ◽

Clark T Sawin

Keyword(s):

Growth Hormone ◽

Human Serum ◽

Serum Insulin ◽

Immunoreactive Insulin ◽

Human Serum Sample ◽

Reference Standard ◽

Insulin Effect ◽

Absolute Value ◽

Standard Curve ◽

Double Antibody

Abstract Human serum can be depleted of insulin and growth hormone by treatment with dextran-coated charcoal or by dialysis, or both. Inclusion of such hormone-depleted serum in the standard curve of a double-antibody radioimmunoassay for immunoreactive insulin more nearly mimics the conditions under which an unknown human serum sample is assayed. Compared to the usual standard curve in which serum albumin is the only protein, the addition of hormone-depleted serum can cause an increase (by an average 64% under the conditions we used) in the absolute value for apparent insulin in serum. The effect of hormone-depleted serum should be tested in the standard curves of double-antibody radioimmunoassays and included routinely in these standard curves if it changes the results. When used in conjunction with an eventual reference standard for human insulin, this modification of the insulin assay may make the measured values of human serum insulin from different laboratories more comparable.

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