Thermal load, especially vertical temperature gradient, is an important factor that should be considered during the bridge design procedure. In the world, several researches focusing on temperature gradient was made, mostly in USA and China, but only a few analyzed actual temperature gradients measuring at European bridges. In this paper, short overview of temperature loading of bridge structures is described. Then, the temperature measurement of prestressed concrete box girder bridge built by launching method is analyzed. Recorded temperature gradient has been compared with temperature gradients for the concrete box girder cross section recommended by Eurocode design specifications.