Determination of fluid–solid transitions in model protein solutions using the histogram reweighting method and expanded ensemble simulations

2004 ◽  
Vol 120 (6) ◽  
pp. 3003-3014 ◽  
Author(s):  
Jaeeon Chang ◽  
Abraham M. Lenhoff ◽  
Stanley I. Sandler
Keyword(s):  
Protein Solutions ◽  
Ensemble Simulations ◽  
Model Protein ◽  
Expanded Ensemble

Analytical characterization of IgG–cTpp and IgG–Mn-cTpp conjugates

2015 ◽  
Vol 19 (11) ◽  
pp. 1177-1184
Author(s):  
Kamila Konopińska ◽  
Mariusz Pietrzak ◽  
Radosław Mazur ◽  
Elżbieta Malinowska
Keyword(s):  
Detection System ◽  
Size Exclusion ◽  
Quantitative Detection ◽  
Measured Signal ◽  
Indirect Determination ◽  
Exclusion Chromatography ◽  
Model Protein ◽  
Spectrofluorimetric Detection ◽  
Secondary Antibodies

Herein, the conjugation of carboxylated tetraphenylporphyrin or its derivative containing manganese cation and model protein — immunoglobulin G is presented. The obtained IgG–cTpp and IgG–Mn-cTpp conjugates were subsequently used for model immunoassays construction. The IgG–cTpp formation was confirmed using size-exclusion chromatography. Thanks to the unique properties of applied labels the assay analysis was carried out with both spectrophotometric and spectrofluorimetric detection. The assays were performed creating semi-quantitative detection system using 96-well plates. The incubation time, ensuring full saturation of the surface with secondary antibodies was also optimized. Moreover, in the case of IgG–Mn-cTpp conjugates we present the possibility of both direct and indirect determination of the label, the latter based on the catalytic activity of Mn-cTpp, which allows for amplification of the measured signal. We proved that both cTpp and Mn-cTpp may be successfully used for protein labeling and serve as universal tracers for various formats of affinity assays and sensors.

Determination of Free Energy from Chemical Potentials: Application of the Expanded Ensemble Method

1996 ◽  
Vol 18 (1-2) ◽  
pp. 43-58 ◽  
Author(s):  
A. P. Lyubartsev ◽  
A. Laaksonen ◽  
P. N. Vorontsov-velyaminov
Keyword(s):  
Free Energy ◽  
Ensemble Method ◽  
Chemical Potentials ◽  
Expanded Ensemble

Experimental Determination of Microsecond Reorientation Correlation Times in Protein Solutions

2013 ◽  
Vol 117 (13) ◽  
pp. 3548-3553 ◽  
Author(s):  
Enrico Ravera ◽  
Giacomo Parigi ◽  
Andi Mainz ◽  
Tomasz L. Religa ◽  
Bernd Reif ◽  
...  
Keyword(s):  
Experimental Determination ◽  
Protein Solutions ◽  
Correlation Times
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