Versatile four-leg thermoelectric module test setup adapted to a commercial sample holder system for high temperatures and controlled atmospheres

2021 ◽  
Vol 92 (4) ◽  
pp. 043902
Author(s):  
Raphael Schuler ◽  
Reshma K. Madathil ◽  
Truls Norby
Author(s):  
Logan M. Compton ◽  
James L. Armes ◽  
Gary L. Solbrekken

Successful cryopreservation protocols have been developed for a limited number of cell types through an extensive amount of experimentation. To optimize current protocols and to develop effective protocols for a larger range of cells and tissues it is imperative that accurate transport models be developed for the cooling process. Such models are dependent on the thermodynamic properties of intracellular and extracellular solutions, including heat capacity, latent heat, and the physical phase change temperatures. Scanning techniques, such as differential-scanning calorimetry (DSC) and differential thermal analysis are effective tools for measuring those thermodynamic properties. It is essential to understand the behavior of the in house fabricated differential-scanning calorimeter given different cooling and warming rates to reassure and validate the obtained experimental results. A 1-D transient CFD code was created in Matlab using Patankar’s theory to not only validate obtained experimental results but aid in optimizing the control system to produce linear cooling and warming rates. A freezing model was also implemented as a subroutine to numerically observe the effect of heat release and absorption of the sample during a run. The numeric model is composed of a multilayer scheme that incorporates a thermoelectric module which provides the primary temperature control along with the micron sized bridge with sample holder and thermocouple. An electric current profile is imported in from either an experimental run to validate results or from an optimization program to determine the optimum electrical current profile for a desired temperature profile. Numeric detection of heat capacity, latent heat, and thermal resistance has also been demonstrated.


2011 ◽  
pp. 587-594 ◽  
Author(s):  
N. Lallu ◽  
J. Burdon ◽  
D. Billing ◽  
P. Pidakala ◽  
K. McDermott ◽  
...  

2010 ◽  
Vol 2010.7 (0) ◽  
pp. 233-234
Author(s):  
Yuta KIMURA ◽  
Takuto KUSHI ◽  
Satoshi WATANABE ◽  
Kazuhisa SATO ◽  
Atsushi UNEMOTO ◽  
...  

2011 ◽  
Vol 198 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Koji Amezawa ◽  
Takuto Kushi ◽  
Kazuhisa Sato ◽  
Atsushi Unemoto ◽  
Shin-ichi Hashimoto ◽  
...  

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 512E-512
Author(s):  
Elhadi M. Yahia ◽  
Dora Ortega ◽  
Pamela Moreno ◽  
Alejandro Martinez

Previous work in our laboratory and also reported in this meeting has indicated that insecticidal controlled atmospheres at high temperatures (0.5% O2 + 50% CO2 at 44-55°C and 50% RH) are very effective in causing in vitro mortality of eggs and third instar larvas of Anastrepaha ludens and A. obliqua. This work is a follow up that evaluated the effect of such atmospheres on the in vivo mortality of third instar larvas artificially infested in mango. Atmospheres evaluated included 0% O2+ 50% CO2 at 35, 37, 39, 40, 42, 43, 44, 45, 46, 47, 48, and 49°C for 160 min. Treatments at 35-40°C caused 100% mortality of larvas of A. obliqua, but not of A. ludens. Temperatures of 42 to 49°C caused 100% mortality of larvas of both species. Statistical analysis to calculate the probit 9 will be discussed.


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