Expression of matrix metalloproteinases and tissue inhibitor of metalloproteinases in laryngeal and pharyngeal squamous cell carcinoma: A quantitative analysis

2000 ◽  
Vol 122 (5) ◽  
pp. 712-716
Author(s):  
Steven P. Magary ◽  
Matthew W. Ryan ◽  
R. W. Tarnuzzer ◽  
Lori Kornberg
Keyword(s):  
Head And Neck ◽  
Squamous Cell ◽  
Normal Tissue ◽  
Squamous Cell Carcinomas ◽  
Chain Reaction ◽  
Primary Tumors ◽  
Qrt Pcr ◽  
Specific Mrnas ◽  
Polymerase Chain ◽  
Pharyngeal Squamous Cell Carcinoma

Squamous cell carcinomas of the head and neck are known for their aggressive growth and propensity to metastasize. Invasion is facilitated by matrix metalloproteineases (MMPs). Tissue inhibitors of MMPs (TIMPs) negatively regulate MMP activity. MMP and TIMP expression in head and neck squamous cell carcinomas was determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). qRT-PCR allows measurement of several mRNAs from as little as 4 μg of total cellular RNA. We measured MMP-1, MMP-2, MMP-9, and TIMP-1 expression in 8 specimens of primary tumors and adjacent normal tissue. MMP-1 was overexpressed in 6 of 8 tumors, and MMP-9 was over-expressed in 4 of 7 tumors. MMP-2 was expressed in 3 of 8 tumors and 3 of 8 normal samples. TIMP-1 was expressed in all specimens. This work demonstrates that qRT-PCR can be used to examine expression of specific mRNAs in clinical specimens. Therefore this method provides another tool for the molecular analysis of tumors.

Expression of Matrix Metalloproteinases and Tissue Inhibitor of Metalloproteinases in Laryngeal and Pharyngeal Squamous Cell Carcinoma: A Quantitative Analysis

2000 ◽  
Vol 122 (5) ◽  
pp. 712-716 ◽  
Author(s):  
Steven P. Magary ◽  
Matthew W. Ryan ◽  
R. W. Tarnuzzer ◽  
Lori Kornberg
Keyword(s):  
Head And Neck ◽  
Squamous Cell ◽  
Normal Tissue ◽  
Squamous Cell Carcinomas ◽  
Chain Reaction ◽  
Primary Tumors ◽  
Qrt Pcr ◽  
Specific Mrnas ◽  
Polymerase Chain ◽  
Pharyngeal Squamous Cell Carcinoma

Squamous cell carcinomas of the head and neck are known for their aggressive growth and propensity to metastasize. Invasion is facilitated by matrix metalloproteineases (MMPs). Tissue inhibitors of MMPs (TIMPs) negatively regulate MMP activity. MMP and TIMP expression in head and neck squamous cell carcinomas was determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). qRT-PCR allows measurement of several mRNAs from as little as 4 μg of total cellular RNA. We measured MMP-1, MMP-2, MMP-9, and TIMP-1 expression in 8 specimens of primary tumors and adjacent normal tissue. MMP-1 was overexpressed in 6 of 8 tumors, and MMP-9 was over-expressed in 4 of 7 tumors. MMP-2 was expressed in 3 of 8 tumors and 3 of 8 normal samples. TIMP-1 was expressed in all specimens. This work demonstrates that qRT-PCR can be used to examine expression of specific mRNAs in clinical specimens. Therefore this method provides another tool for the molecular analysis of tumors.

Human papilloma viruses: a study of their prevalence in the normal larynx

1994 ◽  
Vol 108 (4) ◽  
pp. 319-320 ◽  
Author(s):  
Desmond A. Nunez ◽  
Siân M. Astley ◽  
Fraser A. Lewis ◽  
Michael Wells
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Normal Tissue ◽  
Hpv Infection ◽  
Proteinase K ◽  
Chain Reaction ◽  
Human Papilloma Viruses ◽  
Hpv Types ◽  
Polymerase Chain

AbstractThe association of human papilloma viruses (HPV) with laryngopharyngeal squamous cell carcinoma is under investigation. The suitability of control tissue in the reported series, invariably obtained from histologically normal tissue adjacent to a squamous cell carcinoma or from patients with benign laryngopharyngeal disease, is questionable. The present study determined the prevalence of HPV in a series of normal larynges.Twelve autopsy larynges were collected. DNA was obtained by SDS proteinase K digestion. Evidence of HPV infection was documented by the polymerase chain reaction using oligonulceotide primers complementary to sequences in the E6 region of HPV types 11, 16 and 18.Four female and eight male larynges, mean age 65 years (sd=16 years) were collected 72 hours postmortem (median value). HPV type 11 was isolated from three specimens. A 25 per cent prevalence rate for HPV 11 was found. No other HPV types were isolated.

ATL

2014 ◽  
Vol 24 (2) ◽  
pp. 201-209 ◽  
Author(s):  
Cheng-Chang Chang ◽  
Rui-Lan Huang ◽  
Hui-Chen Wang ◽  
Yu-Ping Liao ◽  
Mu-Hsien Yu ◽  
...  
Keyword(s):  
Squamous Cell ◽  
Methylation Status ◽  
Squamous Cell Carcinomas ◽  
Odds Ratios ◽  
Chain Reaction ◽  
Bisulfite Pyrosequencing ◽  
Specific Polymerase Chain Reaction ◽  
The Status ◽  
Polymerase Chain ◽  
Normal Controls

ObjectiveThis study aimed to investigate the status of DNA methylation of 6 genes,LMX1A,NKX6-1,PAX1,PTPRR,SOX1, andZNF582, previously found from squamous cell carcinomas in adenocarcinomas (ACs) of the uterine cervix.MethodsWe assessed the methylation status of these genes in 40 ACs, cervical scrapings from 23 ACs, and 67 normal control cervices by real-time quantitative methylation-specific polymerase chain reaction. The results were validated by bisulfite pyrosequencing.ResultsThe methylation levels of all the 6 genes in the ACs were significantly higher than those in normal cervical tissues, especially forPAX1,PTPRR,SOX1, andZNF582. The odds ratios and 95% confidence intervals (CIs) of high methylation levels inPAX1,PTPRR,SOX1, andZNF582for the risk of developing an AC were 15.7 (95% CI, 7.0–40.6), 16.9 (95% CI, 7.6–43.0), 32.1 (95% CI, 12.1–124.3), and 25.4 (95% CI, 10.4–78.3), respectively (allP< 0.001). The methylation indices ofPAX1,PTPRR,SOX1, andZNF582recovered from scrapings of ACs were significantly higher than in normal controls. The odds ratios of these indices for the risk of developing an AC inPAX1,PTPRR,SOX1, andZNF582were 6.2 (95% CI, 2.6–15.4), 12.1(95% CI, 3.8–46.4), 6.2 (95% CI, 2.6–15.8), and 20.6 (95% CI, 6.9–77.5), respectively (allP< 0.001).ConclusionsCervical ACs carry aberrantly high methylation rates ofPAX1,PTPRR,SOX1, andZNF582—commonly methylated in squamous cell carcinomas—which might help for AC screening.

Connexins within the rat larynx

2008 ◽  
Vol 139 (6) ◽  
pp. 823-828 ◽  
Author(s):  
Mark B. Van Deusen ◽  
Michael J. Lyon
Keyword(s):  
Polymerase Chain Reaction ◽  
American Academy ◽  
Head And Neck ◽  
Immunohistochemical Staining ◽  
Vocal Folds ◽  
Neck Surgery ◽  
Chain Reaction ◽  
Qrt Pcr ◽  
Laryngeal Mucosa ◽  
Polymerase Chain

Objectives/Hypothesis To determine the types and localization of connexins within the rat larynx. Study Design Quantitative real time polymerase chain reaction (qRT-PCR) of the epiglottis and laryngeal mucosa was used to identify connexins (Cx). Immunohistochemical labeling was then used to localize the Cxs within the larynx. Methods Twelve larynges from 3 to 4 month old Fisher-344 rats were used. RNA was extracted (N = 8) and cDNA produced. Primers for Cx26, Cx30, Cx32, Cx37, Cx40, and Cx43 were added and qRT-PCR performed. Others larynges were serially sectioned for immunohistochemistry. Results qRT-PCR revealed Cx43, Cx32, and Cx30 within the epiglottis and Cx43 in the vocal folds and Cx43 and Cx32 within the subglottic mucosa. Immunohistochemical staining confirmed these results. Conclusion The rat epiglottis is rich in Cx43, Cx32, and Cx30 whereas the vocal folds contain Cx43 and the subglottic mucosa Cx43 and Cx32. Their localizations suggest involvement in secretion for protective purposes and they may play a key role in laryngeal pathoses. © 2008 American Academy of Otolaryngology-Head and Neck Surgery Foundation. All rights reserved.

scholarly journals A Subset of Equine Gastric Squamous Cell Carcinomas Is Associated With Equus Caballus Papillomavirus–2 Infection

2020 ◽  
Vol 57 (3) ◽  
pp. 427-431 ◽  
Author(s):  
Elizabeth Alloway ◽  
Keith Linder ◽  
Susan May ◽  
Trevor Rose ◽  
Josepha DeLay ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Squamous Cell Carcinoma ◽  
Squamous Cell ◽  
Distant Metastases ◽  
Equus Caballus ◽  
Squamous Cell Carcinomas ◽  
Chain Reaction ◽  
Potential Association ◽  
Polymerase Chain

Squamous cell carcinoma (SCC) is the most common neoplasm of the equine stomach. However, the mechanisms underlying malignant transformation are unknown. As Equus caballus papillomavirus–2 (EcPV-2) is a likely cause of some genital SCCs, we hypothesized that EcPV-2 is associated with a subset of equine gastric SCCs. To this aim, we performed polymerase chain reaction (PCR) and in situ hybridization (ISH) for EcPV-2 E6/ E7 oncogenes on 11 gastric SCCs and on gastric samples from 15 control horses with no SCC. PCR for EcPV-2 was positive in 7/11 (64%) gastric SCCs; non-SCC gastric samples were all negative. Intense hybridization signals for EcPV-2 E6/E7 nucleic acid were detected by ISH within tumor cells in 5/11 (45%) gastric SCCs, including distant metastases. No hybridization signals were detected within any of the non-SCC gastric cases. This study provides support for a potential association between EcPV-2 infection and a subset of equine gastric SCC.

scholarly journals Chromoendoscopy to Detect Early Synchronous Second Primary Esophageal Carcinoma in Patients with Squamous Cell Carcinomas of the Head and Neck?

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Pavel Komínek ◽  
Petr Vítek ◽  
Ondřej Urban ◽  
Karol Zeleník ◽  
Magdaléna Halamka ◽  
...  
Keyword(s):  
Esophageal Carcinoma ◽  
Head And Neck ◽  
Squamous Cell ◽  
Squamous Cell Carcinomas ◽  
Second Primary ◽  
Esophageal Mucosa ◽  
Intense Staining ◽  
Primary Tumors ◽  
Flexible Esophagoscopy ◽  
Esophageal Carcinomas

Objective. To evaluate the use of flexible esophagoscopy and chromoendoscopy with Lugol’s solution in the detection of early esophageal carcinomas (second primary carcinomas) in patients with squamous cell carcinoma of the head and neck (HNSCC).Methods. All patients with newly diagnosed HNSCC underwent office-based Lugol's chromoendoscopy. After flexible esophagoscopy with white light, 3.0% Lugol's iodine solution was sprayed over the entire esophageal mucosa. Areas with less-intense staining (LVLs) were evaluated and biopsies taken.Results. 132 patients with HNSCC were enrolled in this study. The most frequent primary tumors were oropharyngeal (49/132), tumors of the oral cavity (36/132), and larynx (35/132). The majority of subjects (107/132 patients, 81.1%) had advanced HNSCC carcinomas (stages III and IV). Multiple LVLs were discovered in 24 subjects (18.2%) and no LVLs in 108 (81.8%) subjects. Fifty-five LVL biopsy specimens were obtained and assessed. Squamous cell carcinomas were detected in two patients, peptic esophagitis in 11 patients, gastric heterotopic mucosa in two patients, hyperplasia in two patients, and low- and high-grade dysplasia in three patients.Conclusion. Although only two patients with synchronous primary carcinomas were found among the patients, esophagoscopy should be recommended after detection of HNSCC to exclude secondary esophageal carcinoma or dysplasia.

Sign in / Sign up

Export Citation Format

Share Document