Diagnostic DNA markers for cereal cyst nematode resistance in bread wheat

2001 ◽  
Vol 52 (12) ◽  
pp. 1367 ◽  
Author(s):  
F. C. Ogbonnaya ◽  
N. C. Subrahmanyam ◽  
O. Moullet ◽  
J. de Majnik ◽  
H. A. Eagles ◽  
...  
Keyword(s):  
Nematode Resistance ◽  
Wheat Breeding ◽  
Primary Objective ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Aegilops Ventricosa ◽  
Breeding Lines ◽  
Marker Selection ◽  
Efficiency Of Selection ◽  
Wheat Lines

The development of cultivars resistant to cereal cyst nematode (CCN) is a primary objective in wheat breeding in the southern wheatbelt of Australia. Nine CCN resistance genes have been identified in wheat and its relatives, some of which confer resistance to the Australian pathotype of CCN (Ha13). Cultivars released in Australia with CCN resistance carry either the Cre1 or CreF gene, with the Cre3 gene present in advanced breeding lines. The biological assay for CCN resistance screening in wheat is time-consuming, not reliable on a single-plant basis, and prone to inconsistencies, thus reducing the efficiency of selection amongst breeding lines. Using gene sequences initially isolated from the Cre3 locus, a DNA-based marker selection system was developed and applied to unambiguously identify wheat lines carrying resistance alleles at theCre1 and/or Cre3 loci in breeding populations derived from diverse genetic backgrounds. Homologues of sequences from the Cre3 locus, located elsewhere in the wheat genome, can also be used to select wheat lines with a newly identified CCN resistance gene (Cre6) introgressed from Aegilops ventricosa. Application of these markers has become an integral part of the southern Australian breeding programs.

scholarly journals How do eyespot resistance genes transferred into winter wheat breeding lines affect their yield?

2016 ◽  
Vol 56 (4) ◽  
pp. 319-322 ◽  
Author(s):  
Michał Kwiatek ◽  
Halina Wiśniewska ◽  
Marek Korbas ◽  
Magdalena Gawłowska ◽  
Jolanta Belter ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Wheat Variety ◽  
Wheat Breeding ◽  
Aegilops Ventricosa ◽  
Wheat Varieties ◽  
Breeding Lines ◽  
Eyespot Resistance ◽  
Limited Effectiveness ◽  
Wheat Lines ◽  
Resistance Expression

Abstract Eyespot can reduce yields, even up to 50%. There are four genetically characterized resistances in wheat varieties, controlled by: (1) the Pch1 gene, transferred from Aegilops ventricosa; (2) the Pch2 gene, originating from wheat variety Capelle Desprez; (3) the Pch3 gene, originating from Dasypyrum villosum; and (4) the Q.Pch.jic-5A gene, a quantitative trait locus (QTL) located on chromosome 5A of Capelle Desprez. However, those loci have drawbacks, such as linkage of Pch1 with deleterious traits and limited effectiveness of Pch2 against the disease. Here we present an initial study which aims to characterize wheat pre-registration breeding lines carrying 12 eyespot resistance genes, consider their resistance expression in inoculation tests and the influence of resistance genotypes on the yield. We selected four groups of breeding lines, carrying: (1) the Pch1 gene alone: one line; (2) the Pch2 gene alone: four lines; (3) the Q.Pch.jic-5A gene alone: one line; and (4) Pch1 + Q.Pch.jic-5A: three lines. For the first time, the effect of the combination of Pch1 and Q.Pch.jic-5A genes was compared with resistance conferred by Pch1 or Q.Pch.jic-5A alone. We found significant differences between infection scores evaluated in resistant lines carrying Pch1 and Q.Pch.jic-5A alone, while no differences in terms of the level of resistance expression were detected between Pch1 alone and Pch1 + Q.Pch.jic-5A, and between wheat lines carrying Pch1 and Pch2 alone. Moreover, we demonstrated that the Pch1 gene, together with an Ae. ventricosa segment, caused statistically significant yield losses, both as a single eyespot resistance source or in a combination with Q.Pch.jic-5A. Yield scores showed that wheat lines with Q.Pch.jic-5A had the highest yields, similar to the yielding potential of Pch2-bearing lines and control varieties.

Molecular mapping of cereal cyst nematode resistance in Triticum monococcum L. and its transfer to the genetic background of cultivated wheat

Euphytica ◽  
2010 ◽  
Vol 176 (2) ◽  
pp. 213-222 ◽  
Author(s):  
Kuldeep Singh ◽  
Parveen Chhuneja ◽  
Inderjit Singh ◽  
S. K. Sharma ◽  
Tosh Garg ◽  
...  
Keyword(s):  
Genetic Background ◽  
Molecular Mapping ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Triticum Monococcum ◽  
Cereal Cyst Nematode

Registration of H192 and H782, White Winter Wheat Lines Resistant to Cereal Cyst Nematode and Powdery Mildew

2016 ◽  
Vol 11 (1) ◽  
pp. 71-74 ◽  
Author(s):  
Dan Qiu ◽  
Lei Cui ◽  
Yanling Sun ◽  
Jingwei Zou ◽  
Chaoxing Zheng ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Winter Wheat ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Wheat Lines

scholarly journals Spring Wheat Tolerance and Resistance to Heterodera avenae in the Pacific Northwest

Plant Disease ◽  
2013 ◽  
Vol 97 (5) ◽  
pp. 590-600 ◽  
Author(s):  
Richard W. Smiley ◽  
Juliet M. Marshall ◽  
Jennifer A. Gourlie ◽  
Timothy C. Paulitz ◽  
Shyam L. Kandel ◽  
...  
Keyword(s):  
Pacific Northwest ◽  
Spring Wheat ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Heterodera Avenae ◽  
Breeding Lines ◽  
The Pacific ◽  
Potential Benefits ◽  
Controlled Environments ◽  
Systematic Field

The cereal cyst nematode Heterodera avenae reduces wheat yields in the Pacific Northwest. Previous evaluations of cultivar resistance had been in controlled environments. Cultivar tolerance had not been evaluated. Seven spring wheat trials were conducted in naturally infested fields in three states over 2 years. A split-plot design was used for all trials. Five trials evaluated both tolerance and resistance in 1.8-by-9-m plots treated or not treated with nematicides. Two trials evaluated resistance in 1-m head rows where each wheat entry was paired with an adjacent row of a susceptible cultivar. Cultivars with the Cre1 resistance gene (‘Ouyen’ and ‘Chara’) reduced the postharvest density of H. avenae under field conditions, confirming Cre1 parents as useful for germplasm development. Ouyen was resistant but it was also intolerant, producing significantly lower grain yield in controls than in plots treated with nematicides. Susceptible cultivars varied in tolerance. Undefined resistance was identified in one commercial cultivar (‘WB-Rockland) and four breeding lines (UC1711, SO900163, SY-B041418, and SY-97621-05). This research was the first systematic field demonstration of potential benefits to be derived through development and deployment of cultivars with resistance plus tolerance to cereal cyst nematode in North America.

Cloning and sequence analysis of the leucine-rich repeats region of cereal cyst nematode resistance gene

2006 ◽  
Vol 3 (3) ◽  
pp. 231-235
Author(s):  
Zhai Xu-Guang ◽  
Liu Yi ◽  
Wu Fang ◽  
Deng Guang-Bing ◽  
Pan Zhi-Fen ◽  
...  
Keyword(s):  
Amino Acid ◽  
Sequence Analysis ◽  
Nucleotide Sequence ◽  
Resistance Gene ◽  
Nematode Resistance ◽  
Cyst Nematode ◽  
Cereal Cyst Nematode ◽  
Coding Region ◽  
Sequence Identity ◽  
Nematode Resistance Gene

AbstractAccording to the sequence of Rccn4, which is highly similar to the nucleotide-binding site (NBS) coding region of the cereal cyst nematode resistance gene, Cre3, three 3′ nested primers were designed to amplify its 3′ flanking region through single oligonucleotide nested polymerase chain reaction (SON-PCR). One 1264 bp band, Rccn-L, was amplified from E-10, a wheat–Aegilops variabilis translocation line containing the cereal cyst nematode resistance gene from Ae. variabilis. Sequence analysis showed that Rccn-L possesses the 3′ flanking sequence of Rccn4 and contains a 55 bp-sized consensus sequence with Rccn4. The coding region was 1026 bp, consisting of an incomplete open reading frame, a terminator codon and no initiation codon and intron; it encoded a peptide of 342 amino acid residues and shared 86% nucleotide sequence identity with Cre3. The peptide had a conserved leucine-rich repeat (LRR) domain, containing the imperfect repeats, XXLXXLXXL, comprising 17% leucine residues, and shares, respectively, 89% nucleotide sequence and 78% amino acid sequence identity with the LRR sequence of the Cre3 locus. In the present study, SON-PCR was used successfully, for the first time, in plant genome research and proved to be a valuable method in plant gene cloning. The acquirement of Rccn-L established the foundation for obtaining the complete Rccn gene and further structural and functional investigations.

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